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Preparation and application of a Polygonatum pectin polysaccharide

A technology similar to polysaccharide and Polygonatum, applied in the field of natural medicinal chemistry and cosmetics, can solve problems such as no detailed research, no report on antioxidant effects, and no detection of the role of skin cells.

Active Publication Date: 2019-03-08
SHANGHAI JAHWA UNITED
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The defect of this application is that it only adopts Polygonatum extract mixture, and the extract is 80% alcohol extract, and it is crude extract
The defect of this document is that: the crude polysaccharide extracted from Huangjing water was used in the article, and the rat tissue extract was used for biochemical test. The effect of the sample on skin cells was not tested, and there was no scientific data support for the development of cosmetics.
[0010] It can be seen that the current anti-aging and anti-oxidation research on Rhizoma Polygonatum is focused on extracting crude polysaccharides from water, and the main polysaccharide components have not been studied in detail to determine the structure of the homogeneous polysaccharides with antioxidant activity.
Simultaneously about this Polygonatum polysaccharide also has no report to the antioxidant effect of skin dermis fibroblast

Method used

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  • Preparation and application of a Polygonatum pectin polysaccharide
  • Preparation and application of a Polygonatum pectin polysaccharide
  • Preparation and application of a Polygonatum pectin polysaccharide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0069] The preparation of embodiment 1 Polygonatum pectin polysaccharide

[0070] (1) Take 2 kg of Polygonatum tuber, soak it in ethanol to degrease, dry naturally at room temperature, extract the dried raw material of Polygonatum tuber with boiling water, and extract 1 kg of medicinal material with 10 L of water according to the ratio of the original medicinal material of Polygonatum and the amount of water added each time. A total of 4 extractions, 4 hours each time.

[0071] (2) Combine the extracts obtained from each extraction, finally concentrate to 5-15L, dialyze against flowing water for three days, concentrate the liquid in the dialysis bag to 5-10L, add 5-10L of 30% (w / v) trichloro Acetic acid solution, after deproteinization, neutralize with alkali to pH 7.0 to 8.0, dialyze against flowing water for three days, concentrate the inner liquid, centrifuge to remove the precipitate, add ethanol to the supernatant for precipitation, stand overnight at 4°C, and obtain a pr...

Embodiment 2

[0074] Example 2 Physicochemical property analysis of Polygonatum pectin polysaccharide PSPW2

[0075] According to the routine test method of polysaccharides, the dextran T-700, T-110, T-80, T-40, T-11 with known molecular weights were used as standard products (produced by Pharmacia Company) for determination. Using Agilent S1200 liquid phase HPGPC (Ultrahydragel500 series Ultrahydragel 2000 column) for analysis, the results are as follows figure 2 shown. Polygonatum pectin polysaccharide PSPW2 obtained in Example 1 is a homogeneous polysaccharide with an average molecular weight of 8-15 KDa.

Embodiment 3

[0076] The sugar composition analysis of embodiment 3 Polygonatum pectin polysaccharide PSPW2

[0077] Take 3 mg of Polygonatum pectin polysaccharide PSPW2 sample of Example 1, hydrolyze it with 2M trifluoroacetic acid for 2 hours at 110°C, and then concentrate the hydrolyzate under reduced pressure at 40°C and evaporate to dryness after cooling, and repeat the operation 4-5 times to remove the residual Reaction of trifluoroacetic acid. Then dissolve the sample with 3ml deionized water, add 20-30mg sodium borohydride, reduce at room temperature for 3 hours (intermittent shaking), then neutralize excess sodium borohydride with 25% acetic acid, until the solution no longer produces bubbles, the pH should be at Between 4 and 5, add methanol several times, evaporate to dryness under reduced pressure, heat at 110°C for 10 minutes, remove residual water, add 3ml of acetic anhydride, seal, react at 100°C for 1 hour, cool, add toluene 4-5 times ( 2-3ml each time) co-evaporated to rem...

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Abstract

Disclosed in the present invention is a Polygonatum sibiricum pectin-type polysaccharide. The Polygonatum sibiricum pectin-type polysaccharide has a main-chain structure formed by connecting a RGI and an HG in an alternating manner and a corresponding weight average molecular weight of 8-15.0 kDa, and the uronic acid content thereof is 0-76.9%, wherein a repeating unit of the RGI is: disaccharide[→4]-α-galacturonic acid-(1→2)-α-rhamnose-(1→)],and a repeating unit structure of the HG is: (1→4)-α-D-galacturonic acid. The present invention further involves a preparation method of the Polygonatum sibiricum pectin-type polysaccharide and the effects thereof.

Description

technical field [0001] The invention relates to the fields of natural medicinal chemistry and cosmetics, more specifically, relates to a pectin polysaccharide extracted and separated from Rhizoma Polygonatum and the reduction and degradation products of the polysaccharide. The invention also relates to a preparation method for extracting and separating the pectin polysaccharide from Polygonatum tuber. In addition, the present invention also relates to the antioxidant application of Polygonatum pectin polysaccharide extracted by the method. Background technique [0002] Aging is a natural process, people will get old, there is no escape from this process. The aging of the human body is first noticed from the skin, manifested as sagging skin, the appearance of wrinkles, and dull skin. In daily life, ultraviolet UVA damages the skin the most. Studies have shown that UVA damages skin cells mainly by producing excessive active oxygen free radicals. [0003] Reactive oxygen fr...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08B37/06A61K8/73A61K31/715A61Q19/00A61P17/16
CPCC08B37/00
Inventor 王滢孙懿丁侃张庆赵亚陈默方程
Owner SHANGHAI JAHWA UNITED
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