Cornea decellularization system and decellularization method

Abstract

The invention relates to the technical field of cornea decellularization, and especially relates to a cornea decellularization system and decellularization method. Automatic control is carried out to reduce artificial intervention in the cornea decellularization process, realize large batch throughput and create conditions for large-scale production and application of corneas. The defects of small batch throughput and restriction of the large-scale production and application of corneas are overcome in the prior art. The cornea decellularization system provided in the embodiment of the invention comprises a decellularization device comprising a reaction tank, and also comprises an oscillation device, a decellularization reagent supply device, an injection water supply device, a waste liquid discharging device and a control device; and the control device is used for controlling the on and off of the oscillation device, controlling the decellularization reagent supply device to supply a decellularization reagent to the reaction tank from, controlling the injection water supply device to supply injection water to the reaction tank and controlling the waste liquid discharging device to discharge a liquid in the reaction tank.

Description

technical field [0001] The invention relates to the technical field of corneal decellularization, in particular to a corneal decellularization system and a decellularization method. Background technique [0002] In recent years, with the in-depth research on artificial cornea and tissue engineered cornea, a breakthrough has been made in seeking a corneal substitute with good biocompatibility, low immunogenicity and good repair effect after eye implantation. Among them, decellularized cornea not only retains the three-dimensional structure of the original tissue, but also its main component is collagen with low immunogenicity, which has the characteristics of good biocompatibility, low immunogenicity and similar structure to human cornea. The best corneal substitute for human keratoplasty. [0003] The existing corneal decellularization technology is mainly completed in the reaction bottle by shaking the shaker. Since the corneal decellularization process requires harsh rese...

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Problems solved by technology

[0003] The existing corneal decellularization technology is mainly completed in the reaction bottle by shaking the shaker. Since the corneal decellularization process requires harsh research and development and production conditions, under the existing test conditions, the batch volume is small, and the corneal decellularization requires The conditions are difficult to meet, which makes it difficult to guarantee the quality of the obtained corneal decellularized cells, which limits the large-scale production and application of corneas.

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