Fixed bud multiplication and plant regeneration method for Elaeagnus mollis Diels

A technology of Samara oil tree and plant, which is applied in the field of fixed bud proliferation and plant regeneration of Samara oil tree, which can solve the problems of low reproduction coefficient, short seed life, low germination rate, etc., to promote the germination of axillary buds and facilitate rooting , the effect of shortening the seedling cycle

Active Publication Date: 2017-01-11
NANJING FORESTRY UNIV
View PDF3 Cites 9 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, under the natural conditions of Samara oil tree, the seed life is short, the germinatio

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Fixed bud multiplication and plant regeneration method for Elaeagnus mollis Diels
  • Fixed bud multiplication and plant regeneration method for Elaeagnus mollis Diels
  • Fixed bud multiplication and plant regeneration method for Elaeagnus mollis Diels

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] A method for fixed bud propagation and plant regeneration of Samara oil tree, comprising the following steps:

[0024] (1) Acquisition of sterile seedlings: Select well-developed mature Samara oil tree fruits, peel off the outer hard shell by physical and mechanical methods, soak in a small amount of detergent and 84 disinfectant for 10 minutes, rinse with running water for 1 hour, and place in a super Soak in 70% alcohol for 40s in a clean table, then use an appropriate amount of 0.1% mercuric chloride and a small amount of Tween~20, shake and soak for 20min, rinse with sterile water for 4~5 times; then remove the inner seed coat of the fruit under a dissecting microscope, place in 3 / 4MS medium, supplemented with 30g / L sucrose and 7g / L agar to obtain full and robust seedlings with axillary buds.

[0025] (2) Selection of bud initiation medium: the seedlings obtained are cut into stem segments with 2 to 4 axillary buds or terminal buds about 3 to 4 cm long, such as fig...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a fixed bud multiplication and plant regeneration method for Elaeagnus mollis Diels. According to the method, on the basis of the characteristic that buds sprout easily from axillary buds of the Elaeagnus mollis Diels, seedlings are obtained by germinating mature Elaeagnus mollis Diels seeds aseptically, tender stem segments with axillary buds or top buds are cut to serve as explants, germination and growth of the axillary buds are promoted with a tissue culture method, healthy and strong shoots with certain degree of lignification are obtained, the purpose of multiplication is realized, and then plant regeneration is realized through rooting culture. With adoption of the fixed bud multiplication method, the problem of high probability of browning occurring in callus and adventitious bud induction processes of a traditional Elaeagnus mollis Diels tissue culture method is solved, and the method is simple to operate and feasible.

Description

technical field [0001] The invention belongs to the technical field of tree tissue culture, and in particular relates to a method for fixed bud proliferation and plant regeneration of Samara oil tree. Background technique [0002] Samara oil tree (Elaeagnusmollis Diels), a perennial deciduous tree or shrub of the genus Elaeagnus mollis, is an ancient plant species unique to China that survived Quaternary glaciers. Samara oil tree seed oil is nutritious, balanced and comprehensive, rich in polyunsaturated fatty acids, of which the content of linoleic acid is as high as 45%. Saturated fatty acids have the function of preventing hypertension, hyperlipidemia, arteriosclerosis and other diseases. The content of vitamin E in the kernel of Samara oil tree is particularly astonishing, as high as 1558.1mg / 100g. Vitamin E is closely related to the growth and development of the human body, and has significant anti-oxidation and anti-aging effects. Samara oil leaves are rich in flavo...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 成铁龙盛宇郑晨陈金慧施季森杨立明明文王光萍
Owner NANJING FORESTRY UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap