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A method suitable for culturing wheat microspores of various genotypes

A technology for microspores and wheat is applied in the field of culturing wheat microspores to obtain regenerated plants, and can solve the problem of high regeneration rate of microspores

Active Publication Date: 2018-08-07
INST OF CROP SCI CHINESE ACAD OF AGRI SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Hu et al. cultured microspores of different genotypes of wheat and their hybrid progeny, and found that the Chris genotype of wheat had better regeneration, but the microspore regeneration rate of Chris and Sinton hybrid progeny was significantly higher than that of the parents

Method used

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  • A method suitable for culturing wheat microspores of various genotypes
  • A method suitable for culturing wheat microspores of various genotypes
  • A method suitable for culturing wheat microspores of various genotypes

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Embodiment 1

[0073] Embodiment 1, cultivating various genotype wheat microspores to obtain regenerated plants

[0074] Wheat varieties to be tested: including varieties widely promoted in production and transgenic recipient materials. Among them, Zhengmai 7698, Zhoumai 22, Aikang 58 and Lunxuan 987 have been widely promoted in production; the transgenic recipient materials include Bobwhite, Fielder, Cadenza, Florida and Kenong 199.

[0075] 1. Plant each tested wheat variety in the greenhouse or in the field.

[0076] 2. At the young ear stage of wheat, select 8 young ears, preferably 2-3 cm from the top of the young ear to the lower end of the flag leaf. figure 1 shown in A and G), 4 ℃ low temperature CuSO 4 Solution (500mg / L) pretreated young ears (soaked) for 10 days.

[0077] 3. Get the young ears of wheat after the pretreatment in step 2, cut off the awns, and sterilize with 5.25% (volume fraction) NaClO for 3 minutes; wash 3 times with sterile water (such as figure 1 shown). Pla...

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Abstract

The invention discloses a method suitable for cultivation of various genotype wheat microspores. A method for cultivating the wheat microspores to obtain regenerated plants, provided by the invention, comprises the following steps: (1) inoculating the microspores of to-be-cultivated wheat to an embryoid induction culture medium and performing induction culture to obtain microspore embryoids; (2) inoculating the microspore embryoids to a regeneration culture medium and performing regeneration culture to obtain regenerated seedlings; (3) inoculating the regenerated seedlings to a rooting culture medium and performing rooting culture to obtain regenerated plants of the to-be-cultivated wheat. The method is suitable for various genotype wheat varieties; furthermore, the embryoid induction ratio, the plant regeneration ratio, the green seedling ratio and the natural doubling ratio of the method are obviously higher than those of the traditional conventional microspore cultivation method. The method has important significance and application value for accelerating wheat haploid breeding and transgenic wheat commercialization progress.

Description

technical field [0001] The invention belongs to the field of plant tissue culture, and relates to a method suitable for culturing wheat microspores of various genotypes, in particular to a method and a special culture medium for cultivating wheat microspores to obtain regenerated plants. Background technique [0002] As one of the most important food crops, wheat is the main intake source of human protein, vitamins, fiber, magnesium, phosphorus and other substances. According to the "World Food and Sustainable Agriculture" conference held in 1997, the world's wheat demand will grow at a rate of 1.6%, reaching 775 million tons in 2020, an increase of 40% over the 552 million tons in 1993. my country's wheat demand in 2020 will be approximately 140-150 million tons, an increase of 46-56 million tons or 49-60% over the current output. Since the founding of the People's Republic of China, more than 2,000 wheat varieties have been cultivated in my country, and the varieties have...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 夏兰琴杜文明孙永伟
Owner INST OF CROP SCI CHINESE ACAD OF AGRI SCI
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