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Gastric adenocarcinoma marker Drosha protein and applications thereof

A gastric adenocarcinoma and protein technology, applied in the field of gastric adenocarcinoma marker Drosha protein, achieves the effect of important clinical application value, simple application method, high specificity and sensitivity

Inactive Publication Date: 2017-02-01
CHONGQING MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, the relationship between Drosha and cancer has aroused great interest of tumor researchers in recent years. The results of many studies by Tchernitsa, Merritt, etc. have shown that Drosha protein is one of the important factors related to tumor prognosis, but the relationship between Drosha and gastric adenocarcinoma is still unclear. No research and patents published

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  • Gastric adenocarcinoma marker Drosha protein and applications thereof
  • Gastric adenocarcinoma marker Drosha protein and applications thereof
  • Gastric adenocarcinoma marker Drosha protein and applications thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Example 1 Correlation analysis between the expression of Drosha protein in cytoplasm and nucleus and the clinicopathology of gastric adenocarcinoma

[0041] Materials: 829 tissue microarray samples of human gastric adenocarcinoma in different pathological stages.

[0042] Methods: The tissue chips of 829 cases of gastric adenocarcinoma were analyzed by immunohistochemical staining, and IPP software was used to detect the expression of Drosha in the cytoplasm and nucleus of the tissue chips.

[0043] Immunohistochemical staining

[0044] 1) Dewaxing: For paraffin-embedded tissue chips, first melt the paraffin in a 60°C incubator, and then put them in xylene I, II, III for 10 minutes respectively;

[0045] 2) Hydration: put the dewaxed tissue chip into 100% alcohol I, II, 95% alcohol, 85% alcohol, 70% alcohol, PBS buffer I, II, III in sequence, each for 5 minutes;

[0046] 3) Block endogenous peroxidase: add 3% H2O2 dropwise to cover the tissue, act at 37°C for 10 minut...

Embodiment 2

[0064] Example 2 Inhibition of the expression of Drosha promotes the invasion of gastric cancer cells

[0065] Material: Gastric cancer cell line SGC-7901.

[0066] Methods: Western blot was used to detect the expression of Drosha protein in SGC-7901 cells before and after siRNA interference.

[0067] Cell culture and cell transfection

[0068] SGC-7901 cells were routinely cultured in RPMI1640 medium containing 10% fetal bovine serum. When the cell fusion was about 60%, according to Roche liposome transfection instructions, siRNA and negative control sequence were transfected into SGC-7901 cells respectively, and protein was collected 48 hours after transfection for detection and Transell invasion experiment.

[0069] In order to further verify the correlation between Drosha and the depth of tumor invasion in Table 1 of Example 1, the expression of Drosha in the gastric cancer cell line SGC-7901 was suppressed with siRNA (see figure 2 ), and then Transwell detected the in...

Embodiment 3 25

[0070] Example 3 Correlation analysis of the prognosis of 225 patients with gastric adenocarcinoma

[0071] In order to further study the relationship between the cytoplasmic expression of Drosha and the prognosis of patients with gastric adenocarcinoma, the relevant prognosis analysis was performed on the 225 patients who were predicted in Example 1. Such as Figure 4 As shown, the Kaplan survival curve analysis showed that the expression level of cytoplasmic Drosha was significantly correlated with the prognosis of adenocarcinoma patients (LR=7.088, P=0.008), while the expression level of nuclear Drosha was not correlated with the prognosis of adenocarcinoma patients (LR=1.954, P=0.162).

[0072] In order to further explore whether cytoplasmic / nuclear Drosha can be used as an independent prognostic factor for patients with gastric adenocarcinoma, COX univariate analysis was used to find out the potential prognostic factors related to the prognosis of patients. As shown in ...

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Abstract

The invention belongs to the biotechnical field, and relates to a gastric adenocarcinoma marker Drosha protein and applications thereof. The applications comprise an application of the Drosha protein in the preparation of a gastric adenocarcinoma diagnosis and treatment reagent, and an application of the Drosha protein in assessment of gastric adenocarcinoma prognosis and / or screening of gastric adenocarcinoma medicines. A kit used for assessing gastric adenocarcinoma prognosis and / or screening the gastric adenocarcinoma medicines comprises an antibody specifically binding to the Drosha protein, and a reagent specifically binding to the Drosha protein. In an application method, the cytoplasm expression level and / or the expression mode of the Drosha protein used as a marker in a pathologic sample is negatively correlated with the grading of the gastric adenocarcinoma, the high expression of the Drosha protein can be used as an independent prognosis factor used for judging the gastric adenocarcinoma human prognosis to be good, and a compound to be tested can be combined with the Drosha protein to detect whether the compound can promote or inhibit the expression of the protein or not in order to screen effective gastric adenocarcinoma medicines, so the Drosha protein has clinic application values.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to a gastric adenocarcinoma marker Drosha protein and application thereof. Background technique [0002] Gastric adenocarcinoma is a type of gastric cancer, which is caused by malignant transformation of gastric gland cells, so it is called adenocarcinoma. The incidence of gastric adenocarcinoma accounts for 95% of gastric malignant tumors. Gastric adenocarcinoma is not sensitive to radiotherapy and chemotherapy, but it can be used as an auxiliary method for the treatment of gastric adenocarcinoma. The incidence of gastric cancer varies around the world. For example, Japan, Chile, and Iceland have very high incidence rates. In the United States, gastric cancer is most common among northerners, the poor, and blacks, but its incidence has decreased to about 8.1 million, and it is the seventh leading cause of cancer death. However, in Japan, the incidence of gastric cancer has decreased, b...

Claims

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Application Information

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IPC IPC(8): G01N33/574G01N33/573G01N33/94
CPCG01N33/573G01N33/57446G01N33/94G01N2333/922
Inventor 柳满然侯懿烜张海龙
Owner CHONGQING MEDICAL UNIVERSITY