Optical path regulating device of flow cytometry under liquid-path-free condition and method

Abstract

The invention provides an optical path regulating device of a flow cytometry under the liquid-path-free condition. The optical path regulating device comprises an illumination light source, an illumination light spot shaping optical path, a standard micro ball rotating device, a non-forward direction scattering light beam shaping optical path, a non-forward direction scattering light detection circuit, a forward scattering light and fluorescent light beam shaping optical path, a forward scattering light detection circuit, a multi-color fluorescent light splitting optical path and a multi-passage fluorescent detection circuit, wherein the illumination light source provides an excitation light source for fluorescence excitation; the illumination light spot shaping optical path is used for compressing light source light beams into illumination light spots of a certain dimension; the standard micro ball rotating device is used for enabling a circular disc loading standard micro balls to rotate so as to simulate single cells to gradually pass through the illumination light spots; the non-forward scattering light beam shaping optical path is used for focusing the non-forward scattering light beams in a certain range; the non-forward scattering light detection circuit is used for performing photoelectric conversion on the non-forward scattering light; the parameter extraction is realized on generated electric pulse signals.

Description

technical field [0001] The invention relates to the field of optical path adjustment and microsphere measurement of a flow cytometer, in particular to an optical path adjustment device and method without a liquid path. Background technique [0002] In the flow cytometer optical path adjustment system, it includes the adjustment of the illumination spot and the spectroscopic collection of the excitation light signal. Since the detection area of ​​the microspheres in the flow cytometer is located at the intersection of the irradiation spot and the direction of the liquid flow, and the instability of the liquid system will cause differences in the relative positions of the microspheres entering the detection area, resulting in the microspheres in the detection area The excitation degree of the irradiation is not the same, resulting in large differences in the intensity of forward scattered light, non-forward scattered light and fluorescence signal of each channel. The pulse pa...

AI Technical Summary

Problems solved by technology

Since the detection area of ​​the microspheres in the flow cytometer is located at the intersection of the irradiation spot and the direction of the liquid flow, and the instability of the liquid system will cause differences in the relative positions of the microspheres entering the detection area, resulting in the microspheres in the detection area The excitation degree of the irradiation is not the same, resulting in large differences in the intensity of forward scattered light, non-forward scattered light and fluorescence signals of each channel

The pulse parameter information obtained by the subsequent detection circuit is inaccurate

The existing optical adjustment system is completely dependent on the high-precision liquid circuit control system, which has poor independence

The hydraulic control system is extremely complex and cumbersome, and there is no standard index for the verification method of control accuracy and laminar flow effect

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