A kind of rapid propagation method of moss wrinkled leaves

Abstract

The invention discloses a rapid propagation method for Brachythecium kuroishicum. The rapid propagation method comprises the following steps: acquiring the stem leaf gametophyte and sporangium of Brachythecium kuroishicum in field and soaking the stem leaf gametophyte and sporangium in sterile water for 5 h; cleaning the gametophyte via flushing, then cutting the gametophyte into small sections with a length of 1 cm, sterilizing the small sections with sodium hypochlorite in a small sterile culture dish with a diameter of 5 cm for 1 min, carrying out cleaning with sterile water five times, then carrying out grinding and crushing, inoculating a BCD medium with the obtained powder, sterilizing the sporangium in a centrifuge tube with a volume of 1.5 ml for 5 min, crushing the sporangium with tweezers and then inoculating the BCD medium with the crushed sporangium; allowing the gametophyte and sporangium to grow in an incubator for two weeks, wherein the incubator has a temperature of 23 DEG C, light intensity of 80 [mu]mol of photons per square meter per second and sunshine duration of 16 h; and after protonemata grow out from the gametophyte and sporangium, carrying out mechanical grinding and crushing, inoculating another BCD medium with the crushed protonemata and carrying out culturing, wherein protonemata propagated in quantity can be obtained in one week, and mature gametophyte can be obtained in one month. The rapid propagation method provides technical guarantee for later industrial production of Brachythecium kuroishicum.

Description

[0001] Field [0002] The invention belongs to the field of biological technology, and in particular relates to a method for aseptic and rapid propagation of the protonema and gametophyte of Physcomitrella rugosa. Background technique [0003] Moss is a group of higher plants transitioning from aquatic to terrestrial. There are about 21,200 species in the world. They are distributed in every corner of the earth except the ocean, and even grow on deserts, tundra and rocks. They are resistant to drought, cold and Barren and highly adaptable, it is the pioneer and pioneer of nature (Kidron, 2014; Cao Tongping, 2014). Yunnan is particularly rich in moss resources, with about 36% of the world's moss species. The survey of plant resources in the areas where large-scale copper mines and gold mines have seriously damaged the ecological environment in many provinces in my country found that mosses, as pioneer plants, grow in places where these vascular plants cannot survive. For exam...

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Problems solved by technology

It is difficult to realize large-scale artificial reproduction, and there is no report on any relevant technical methods

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