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Application of nucleolin to preparation of diagnostic reagent and therapeutic for gastric cancer

A technology of diagnostic reagents and diagnostic kits, applied in the biological field, can solve the problems of low sensitivity and specificity, difficulty in early diagnosis of gastric cancer, etc.

Inactive Publication Date: 2017-02-15
AFFILIATED HUSN HOSPITAL OF FUDAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In current clinical practice, the more common and routine examination is serum blood examination, serum CEA, CA19-9, CA50, CA125 and other tumor-associated antigens can be elevated in patients with gastric cancer, but their sensitivity and specificity are not high
At the same time, most patients with gastric cancer have no obvious clinical symptoms in the early stage, which has caused certain difficulties in the early diagnosis of gastric cancer; therefore, it has become a researcher in this field to find effective markers of gastric cancer and accurately judge the clinical stage and prognosis of the disease. focus of attention

Method used

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  • Application of nucleolin to preparation of diagnostic reagent and therapeutic for gastric cancer

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Example 1 ELISA detection of nucleolin in the plasma samples of patients with gastric cancer and normal controls

[0031] Reagents: ELISA kits were purchased from Uscn Life Science Company.

[0032] Quantitative determination of nucleolin content in human plasma by double antibody ELISA method:

[0033] Perform corresponding operations according to the kit instructions;

[0034] Patients and samples:

[0035] The preoperative blood samples of 28 patients newly diagnosed with gastric cancer between December 2012 and December 2014 were randomly selected, and the blood samples of 28 healthy patients were selected as controls. All blood sample collection protocols were approved by the Ethics Committee of Huashan Hospital.

[0036] 1. Specimen Collection and Maintenance

[0037] Centrifuge the patient's blood sample at 1000×g for 20 minutes, take the supernatant (plasma) and store it at -20°C or -80°C;

[0038] 2. Operation steps:

[0039] 1) Preparation of standards, ...

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Abstract

The invention relates to multi-functional ribosomal protein nucleolin and application thereof, specifically to application of nucleolin as a molecular marker for the gastric cancer of human beings and application of nucleolin to preparation of a preparation or kit used for detecting and diagnosing the gastric cancer of human beings and a therapeutic for the gastric cancer, belonging to the field of biotechnology. According to the invention, ELISA detection is carried out on both the blood plasma sample of a patient with the gastric cancer and a normal control blood plasma sample; detection results show that the level of nucleolin in the blood plasma of the patient with the gastric cancer is substantially higher than the level of nucleolin in the normal control blood plasma sample and that nucleolin is highly expressed in the gastric cancer and secreted to blood; thus, nucleoli can be used as a noninvasive marker for diagnosis of the gastric cancer. The invention further provides application of nucleolin to preparation of a diagnostic reagent and kit for the gastric cancer and a drug with nucleolin as a target and for treatment of the gastric cancer. The invention provides a novel assistant determination means for early diagnosis and early warning of the gastric cancer.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to a multifunctional non-ribosomal protein nucleolin and its use, in particular to the application of nucleolin as a human gastric cancer molecular marker and its preparation or kit for detecting or diagnosing human gastric cancer and Use in therapeutic medicine. Background technique [0002] The prior art discloses that gastric cancer is one of the most common malignant tumors, with about 1 million new cases of gastric cancer each year, and its mortality rate is second only to lung cancer in the world, ranking second in the cancer mortality rate. Gastric cancer is the leading cause of death in digestive tract tumors every year in my country. Studies have shown that the prognosis of gastric cancer is closely related to the stage of the disease, and early diagnosis and treatment can significantly improve the prognosis and prolong the survival period. In current clinical practice, serum b...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68G01N33/574
CPCG01N33/68G01N33/57446
Inventor 刘杰张骏李文帅
Owner AFFILIATED HUSN HOSPITAL OF FUDAN UNIV
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