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A method of treating dye wastewater by using marine Aspergillus niger mycelium and airlift reactor

An air-lift reactor and marine Aspergillus niger technology, applied in chemical instruments and methods, biological water/sewage treatment, water/sludge/sewage treatment, etc., can solve clogged reactors, high cost, and low reuse rate and other problems, to achieve strong salt and acid resistance, reduce treatment costs, and improve treatment effects

Active Publication Date: 2019-08-27
ZHEJIANG UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] (2) The design of the reactor is the key to whether the mycelium ball can be put into practical use, and most of the existing researches are carried out in shake flasks, the scale is small and the cost is high, and there is still a long distance from the actual application, so It is necessary to design and manufacture more reasonable bioreactors to achieve scale expansion;
[0007] (3) The existing mycelium ball treatment sewage technology often has a low reuse rate, or it is difficult to achieve the initial decolorization efficiency after the mycelium ball is reused. Therefore, it is necessary to establish an efficient batch operation method to achieve Efficient reuse of mycelium balls to further reduce wastewater treatment costs
Aspergillus niger mycelium balls are fluffy spheres on the surface. Although they have a large specific surface area, which is very conducive to adsorption, they have weaknesses such as low mechanical strength and easy agglomeration between mycelium balls.
The mass transfer efficiency of the stirred tank reactor is very high, but the shear force generated by the stirred tank is relatively large, which may easily cause the mycelial balls to break; fixed bed and fluidized bed may easily cause the mycelial balls to agglomerate and eventually block the reactor

Method used

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  • A method of treating dye wastewater by using marine Aspergillus niger mycelium and airlift reactor
  • A method of treating dye wastewater by using marine Aspergillus niger mycelium and airlift reactor

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preparation example Construction

[0042] 1. Preparation of marine Aspergillus niger mycelium balls

[0043] Take out the solid slope containing Aspergillus niger (Aspergillus niger) ZJUBE-1CCTCC M 2010132 stored at 4°C, and use sterile water to make a concentration of n=10 6 ~10 9 mL -1 Inoculate the spore suspension of 1-3% into a pre-prepared 500mL special Erlenmeyer flask containing 100mL mycelium culture solution, and culture it on a shaking table at 160-180rpm and 25-35°C for 3-5 days; then, wash off the excess mycelium with water, collect the mycelium balls by filtration, and put them in the refrigerator for later use.

[0044] Medium for preparing mycelial balls: glucose 10-15g / L, NH 4 Cl 2~3g / L, KH 2 PO 4 2~3g / L, MgSO 4 ·5H 2 O 0.5~1.0g / L, yeast extract 2~3g / L, pH 5.0~6.0.

[0045] (2) The structure of the airlift reactor

[0046] Such as figure 2 As shown, the main body of the airlift reactor adopted in the present invention is a circular glass column with a diameter of 6.5cm and a height ...

Embodiment 1

[0055] A kind of method utilizing marine Aspergillus niger mycelia ball and airlift reactor to process dye waste water, specifically as follows:

[0056] (1) Take out the solid slant of Aspergillus niger (Aspergillus niger) ZJUBE-1CCTCC M 2010132, and make it into a concentration of n=10 with sterile water 7 mL -1 Put the spore suspension of 2% into a pre-prepared 500mL special Erlenmeyer flask containing 100mL of mycelia culture solution, culture it on a shaker at 160rpm and 28°C for 3 days; then dissolve the excess bacteria with water Wash off the filaments, collect the mycelium balls by filtration, and put them in the refrigerator for later use.

[0057] Medium for preparing mycelial balls: glucose 10g / L, NH 4 Cl2g / L, KH 2 PO 4 2g / L, MgSO 4 ·5H 2 O0.5g / L, yeast extract 2g / L, pH 5.5.

[0058] (2) Weigh 30g (wet weight) of mycelia balls into the airlift reactor, add 300mL of tap water until the liquid level reaches the lowest waste water outlet (i.e. waste water outlet...

Embodiment 2

[0061] A kind of method utilizing marine Aspergillus niger mycelia ball and airlift reactor to process dye waste water, specifically as follows:

[0062] (1) Take out the solid slant of Aspergillus niger (Aspergillus niger) ZJUBE-1CCTCC M 2010132, and make it into a concentration of n=10 with sterile water 7 mL -1 Put the spore suspension of 2% into a pre-prepared 500mL special Erlenmeyer flask containing 100mL of mycelia culture solution, culture it on a shaker at 160rpm and 28°C for 3 days; then dissolve the excess bacteria with water Wash off the filaments, collect the mycelium balls by filtration, and put them in the refrigerator for later use.

[0063] Medium for preparing mycelial balls: glucose 10g / L, NH 4 Cl2g / L, KH 2 PO 4 2g / L, MgSO 4 ·5H 2 O0.5g / L, yeast extract 2g / L, pH 5.5.

[0064] (2) Weigh 30g (wet weight) of mycelia balls into the airlift reactor, add 300mL of tap water until the liquid level reaches the lowest waste water outlet (i.e. waste water outlet...

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Abstract

The invention discloses a method for treating dye wastewater by utilizing marine aspergillus niger mycelium pellets and an airlift reactor. The method comprises the following steps: obtaining mycelium pellets of marine aspergillus niger through culture; inoculating the mycelium pellets into the airlift reactor to carry out intermittent treatment of multiple batches of dye wastewater, wherein after treatment of each batch is finished, a carbon source and a nitrogen source are added to the airlift reactor and are used for culturing the mycelium pellets, so that the decoloring power of the mycelium pellets is recovered; treatment of the next batch of dye wastewater is carried out again after culture is finished. The process for carrying out semicontinuous wastewater treatment by utilizing the marine aspergillus niger mycelium pellets and combining with the matched airlift reactor has the beneficial effects that by adding the step of culture to recover the decoloring power of the mycelium pellets among all batch treatment processes, the mycelium pellets are taken full advantage of, thus reducing the treatment cost and obviously improving the dye wastewater treatment effects.

Description

technical field [0001] The invention relates to the technical field of dye wastewater treatment, in particular to a method for treating dye wastewater by using marine Aspergillus niger mycelia balls and an airlift reactor. Background technique [0002] The treatment of dye wastewater has always been one of the core issues of environmental protection. Although traditional treatment methods, such as flocculation, ion exchange, advanced oxidation technology, and activated carbon adsorption, have high removal efficiency for specific pollutants, there are problems such as large amount of toxic chemicals, expensive equipment, high operating costs, and limited scope of application. Defects in this aspect have greatly restricted its application. Compared with traditional physical and chemical methods, biological treatment is a more economical treatment. [0003] Among them, fungi are a good choice in the process of sewage treatment. Because there are a large number of functional ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C02F3/32
Inventor 姚善泾陆涛蔡礼年张其磊
Owner ZHEJIANG UNIV