Pure white hypsizigus marmoreus strain, and molecular marker, specific primer pair and application thereof
A technology of molecular markers and shimeji mushrooms, which is applied in the direction of fungi, biochemical equipment and methods, microorganisms, etc., and can solve the problems that strains are prone to degeneration and mutation
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Embodiment 1
[0066] Example 1 Polyspore selfing obtains Finc-W-5
[0067] The strain Finc-W-5 of pure white jiji mushroom was obtained by polyspore self-breeding method.
[0068] Specific steps are as follows:
[0069] 1) The parent strain H-W was purchased from the market;
[0070] 2) Prepare mother seed culture medium, original seed culture medium and cultivar culture medium: the formulations of each culture medium have been given, and will not be repeated here;
[0071] 3) Isolation of basidiospores: the parent strain H-W was placed in a petri dish, and after 24 hours, the spores falling on the petri dish were collected, diluted to an appropriate concentration in sterile water, and spread on a PDA medium plate. Cultivate at 23°C for 10 days, and inoculate the spores on the PDA inclined test tubes with inoculation loops after germination. The tubes were incubated at 23°C for 12 days, after which hyphae were isolated from the tubes and observed under a microscope for the formation of l...
Embodiment 2
[0075] Embodiment 2 Antagonism experiment
[0076] The Finc-W-5 of the present invention and the parent bacterial strain H-W are test materials, and the medium used is PDA medium.
[0077] Finc-W-5 and the parental strain H-W were inoculated and cultured on the plate medium respectively, the inoculation points were 2.5cm apart, and cultured at a constant temperature of 23°C for 18-20 days. Please refer to Table 1 for the results of this experiment.
[0078] Table 1 Antagonism test between different pure white mushroom strains
[0079] Variety H-W Finc-W-5 antagonistic
[0080] like Figure 1-2 As shown, there are obvious antagonistic lines between the junctions of the confrontation culture colonies, indicating that Finc-W-5 is a pure white P.
Embodiment 3
[0081] Embodiment 3 cultivation experiment
[0082]Finc-W-5 of the present invention and parent bacterial strain H-W are respectively cultivated and cultivated, and 4 bacterial ages are set from 70 days to 85 days, and every increase of 5 days is a bacterial age, and each bacterial age is 96 bottles, and then cultivated in small batches The results were summarized and compared, and the advantages and characteristics of the bacterial strain Finc-W-5 of the present invention were summarized.
[0083] The culture medium of this embodiment participates in the cultivar culture medium, and will not be repeated here.
[0084] Cultivation bottle volume: 850cc, bottle mouth diameter 58mm.
[0085] Cultivation: Cultivate for 70-85 days at a temperature of 18-23°C, a relative air humidity of 65-75%, and a carbon dioxide concentration of 2000-3500ppm;
[0086] Cultivation: The fruiting temperature is set at 13°C-15°C, the relative air humidity is 93%-100%, and the carbon dioxide concent...
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Abstract
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