Unlock instant, AI-driven research and patent intelligence for your innovation.
A kind of Corynebacterium glutamicum strain and its application in fermentative production of L-tryptophan
What is Al technical title?
Al technical title is built by PatSnap Al team. It summarizes the technical point description of the patent document.
A technology of Corynebacterium glutamicum, tryptophan, applied in the direction of fermentation, microorganism-based methods, bacteria, etc., can solve problems such as high price
Active Publication Date: 2019-09-24
江苏东誉绿塑生物科技有限公司
View PDF1 Cites 3 Cited by
Summary
Abstract
Description
Claims
Application Information
AI Technical Summary
This helps you quickly interpret patents by identifying the three key elements:
Problems solved by technology
Method used
Benefits of technology
Problems solved by technology
However, due to the high price of L-tryptophan precursors such as serine or L-indole acetic acid, this method is rarely used to produce L-tryptophan at present.
Method used
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
[0081] 1. Breeding of Corynebacterium glutamicum FNT112
[0082] 1. The starting strain Corynebacterium glutamicum ATCC 21851 was inoculated on the slant medium, and activated for 18 hours at 33°C.
[0083] 2. Inoculate the strain activated in step 1 into 40ml of LBG medium, shake at 220r / min, and culture at 33°C for 9h.
[0084] 3. Inoculate 0.8ml of the bacterial solution cultivated in step 2 into a 250ml Erlenmeyer flask containing 40ml of LBG medium, add 1ml of glycine (10g / 100ml), and continue to incubate at 220r / min at 33°C for 9h.
[0085] 4. Collect the bacterial solution in step 3, take 10mL into a sterilized 50ml centrifuge tube, centrifuge at 4000r / min for 15min, discard the supernatant, and collect the bacterial cells.
[0086] 5. Weigh 0.15g lysozyme and 0.05g cellulase, dissolve them in 10ml protoplast stabilization solution (SMM), after about 10min, centrifuge at 4000r / min for 15min after...
Embodiment 2
[0094] Embodiment 2, the fermentation application of Corynebacterium glutamicum FNT112
[0095] 1. Production of L-tryptophan by shake flask fermentation
[0096] 1, the Corynebacterium glutamicum FNT112 that embodiment 1 is obtained is inoculated in the 250mL shaking flask that 30mL seed culture medium is housed, 8 layers of gauze are sealed, 33 ℃ of shaking cultures (220r / min) 16 hours, obtain seed liquid (seed OD of liquid 562nm = 40).
[0097] 2. Inoculate 3 mL of the seed liquid obtained in step 1 into a 250 mL Erlenmeyer flask containing 30 mL of fermentation medium, seal with 8 layers of gauze, and culture at 33° C. with shaking (220 r / min) for 48 hours.
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More
PUM
Login to View More
Abstract
The invention discloses a Corynebacterium glutamicum strain and its application in fermenting and producing L-tryptophan. The invention provides a Corynebacterium glutamicum FNT112, whose preservation number in the China Center for Type Culture Collection is CCTCC NO: M2016398. The invention also protects the culture method and application of Corynebacterium glutamicum FNT112. The Corynebacterium glutamicum FNT112 provided by the present invention can be directly fermented to obtain L-tryptophan, and the content of L-tryptophan in the fermentation liquid of industrial production fermentation is as high as 40.5g / L. The invention has great application value and industrial prospect.
Description
technical field [0001] The invention relates to the field of biotechnology, in particular to a Corynebacterium glutamicum strain and its application in fermentative production of L-tryptophan. Background technique [0002] L-tryptophan (L-valine) is one of the eight essential amino acids for the human body, and it is also an aromatic amino acid. It has significant functions in the metabolic process of the human body and various animals. All play an important role, and are widely used in processed feed, health food, formula diet, and amino acidmedicine. [0003] The production methods of L-tryptophan mainly include extraction method, chemical synthesis method and fermentation method. The extraction method and the chemical synthesis method are difficult to realize industrial production due to the limited source of raw materials, high production cost, low yield and serious pollution. The production of L-tryptophan by microbial fermentation has the advantages of low raw mater...
Claims
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More
Application Information
Patent Timeline
Application Date:The date an application was filed.
Publication Date:The date a patent or application was officially published.
First Publication Date:The earliest publication date of a patent with the same application number.
Issue Date:Publication date of the patent grant document.
PCT Entry Date:The Entry date of PCT National Phase.
Estimated Expiry Date:The statutory expiry date of a patent right according to the Patent Law, and it is the longest term of protection that the patent right can achieve without the termination of the patent right due to other reasons(Term extension factor has been taken into account ).
Invalid Date:Actual expiry date is based on effective date or publication date of legal transaction data of invalid patent.
Login to View More 
Login to View More