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Method for rapidly detecting conduction rule of Aquilaria agallocha inducer in body of Aquilaria agallocha yielding plant and application of method

An agarwood inducer and agarwood technology, which are applied in the biological field, can solve the problems of difficulty in determining the transmission distance and location of the agarwood inducer, time and energy consumption, pale color, etc., so as to reduce the death of the whole plant and save time and cost. , the effect of improving the aroma production rate

Active Publication Date: 2017-03-01
INST OF MEDICINAL PLANT DEV CHINESE ACADEMY OF MEDICAL SCI HAINAN BRANCH
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  • Abstract
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Agarwood inducers are generally colorless liquids. Even if pigment or colored agarwood inducers are added, the color will become very light or even colorless under the purification and dilution of the Agarwood tree's own transport system. Therefore, it is difficult to determine the transport distance and transport site of the agarwood inducer in the tree
After the agarwood inducer enters the tree body, it will cause discoloration of the internal wood, and the discolored part will soon form the place of agarwood. Therefore, in the past, the transmission distance and transmission position of the agarwood inducer in the tree were mainly determined based on the discoloration of the wood. , but wood discoloration generally occurs 7 days or even 10 days after the agarwood inducer enters the tree, so it takes a lot of time and effort to determine the transport law of the agarwood inducer in the tree

Method used

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  • Method for rapidly detecting conduction rule of Aquilaria agallocha inducer in body of Aquilaria agallocha yielding plant and application of method
  • Method for rapidly detecting conduction rule of Aquilaria agallocha inducer in body of Aquilaria agallocha yielding plant and application of method
  • Method for rapidly detecting conduction rule of Aquilaria agallocha inducer in body of Aquilaria agallocha yielding plant and application of method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] Example 1 Rapid detection of the law of agarwood inducer delivery in the infusion method

[0045] (1) Infusion: Calculated by mass percentage, mix 1% methyl jasmonate and 1% ferrous sulfate at a ratio of 1:10 to prepare the agarwood inducer, then add 0.01g ferric chloride to the prepared 100ml agarwood inducer In, that is, the mass concentration of ferric chloride contained in the agarwood inducer is 0.01%.

[0046] Choose 3-year-old trees with a DBH of 8 cm, drill an infusion hole 5 cm deep perpendicular to the trunk at a distance of 45 cm from the ground, and introduce 100 ml of the agarwood inducer containing ferric chloride through the infusion hole through the infusion method. White wood incense tree body, and then seal the hole.

[0047] (2) Color development: After the infusion of the agarwood inducer is completed, the stems or branches at different distances from the infusion hole are cut off with a sharp hacksaw, and then freshly prepared acidic potassium ferr...

Embodiment 2

[0049] Example 2 Rapid detection of the conduction law of the agarwood inducer in the infusion method

[0050] (1) Infusion: Add 0.5g ferrous chloride to 100ml of agarwood inducer, the preparation of this agarwood inducer is calculated by mass percentage: 0.1% methyl jasmonate, 0.5% sodium chloride and 0.3% phenylethanol solution Mixed according to 1:5:3.

[0051] Select a 5-year-old tree with a DBH of 10cm, and drill an infusion hole with a depth of 0.6cm and a depth of 7cm perpendicular to the trunk at a distance of 100cm from the ground, and prepare 100ml of ferrous chloride containing 0.5% The agarwood inducer is slowly injected into the tree through the infusion hole.

[0052] (2) Color development: After the infusion of the agarwood inducer is finished, the stems or branches at different distances from the infusion hole are cut off with a sharp hacksaw, and the freshly prepared 2% potassium ferricyanide solution is dripped on the section, as figure 2 As shown, the pos...

Embodiment 3

[0053] Example 3 Rapid detection of agarwood inducer transport law in high-pressure injection method

[0054] (1) Infusion: add 0.2g ferric chloride to 100ml agarwood inducer, the preparation of this agarwood inducer is calculated by mass percentage: 0.04% gibberellin and 1% sodium bisulfite solution are mixed at 1:25 made by mixing.

[0055] Choose a 5-year-old tree with a DBH of 12cm, and drill a 7cm infusion hole at a distance of 150cm from the ground. Use 6HZ-W1020 type high-pressure trunk injection machine to inject 100ml of agarwood inducer containing 0.2% ferric chloride into the trunk of Akiras sinensis through the infusion hole.

[0056] (2) Color development: After the injection is completed, cut off the stems or branches at different distances from the infusion hole with a sharp hacksaw, and freshly prepared acid potassium ferrocyanide (4% potassium ferrocyanide and 4% hydrochloric acid in equal amounts), drop it on the cross-section, the position where the agarwo...

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Abstract

The invention provides a method for rapidly detecting a conduction rule of an Aquilaria agallocha inducer in a body of an Aquilaria agallocha yielding plant tree and an application of the method. According to the method, the detection time can be shortened greatly and can be shortened to be less than 1 hour from 7 days. According to the method provided by the invention, an Aquilaria agallocha inducer solution containing inorganic salt ions to be detected is transfused into the body of the Aquilaria agallocha yielding plant tree by a transfusion method; and after the transfusion ends, chromogenic detection is carried out on positions, which are away from a transfusion hole by different distances, by adopting a chromogenic reagent of the inorganic salt ions to be detected. According to the method and the application thereof, the operation is simple, convenient and quick, so that the method and the application thereof have an important significance in the research and industrialization of an Aquilaria agallocha formation mechanism.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a method and an application for rapidly detecting the conduction rule of an agarwood inducer in an agarwood-producing plant tree. Background technique [0002] Agarwood is the resinous wood of Aquilaria plants in the Thymelaeaceae family. The formation of Aquilaria is very special. Healthy Aquilaria plants cannot produce agarwood substances, and can only be damaged by external damage such as physical damage, chemical damage, and fungal infection. Afterwards, the aroma is induced. In order to increase the yield of agarwood, people have invented and summarized a variety of fast incense-setting methods. Traditional methods such as "slashing method", "boring method" and "fire-burning method", etc., but these methods are low in efficiency. , and unstable. At present, the area of ​​use is relatively large, and the artificial fragrance method of the "infusion method" is better...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G7/06
CPCA01G7/06
Inventor 刘培卫魏建和杨云张玉秀陈波
Owner INST OF MEDICINAL PLANT DEV CHINESE ACADEMY OF MEDICAL SCI HAINAN BRANCH
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