A kind of tissue culture method of pine pine
A technique of tissue culture of pine pine, which is applied in the field of plant biology, can solve problems such as easy browning and difficult rooting, and achieve the effect of increasing induction rate and rooting rate, high induction rate and rooting rate, and reducing degradation rate
Active Publication Date: 2018-05-22
INNER MONGOLIA M GRASS ECOLOGY & ENVIROMENT GRP CO LTD +1
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[0004] In recent years, there have been reports in the literature about the tissue culture of Pine japonica, but the explants of the pine pine tissue culture reported in the literature mostly select seeds, cotyledons or stem segments, and the culture medium mainly adopts MS medium, but such Very easy to cause browning, and not easy to root
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[0017] Statistical calculation formula in the embodiment
[0018] Survival rate = (number of viable explants / total number of inoculated explants) × 100%
[0019] Primary bud induction rate=(the number of explants that germinated / the total number of inoculated explants)×100%
[0020] Subculture multiplier = (the number of clustered buds induced / the total number of buds inserted into a single plant) × 100%
[0021] Rooting rate=(number of explants of differentiated roots / total number of inoculated explants)×100%
[0022] (1) selection and sterilization of explants, take flower buds that will open 2-3 days before flowering, rinse with tap water for 30-40min, and rinse in 70-75% alcohol for 1-2min after absorbing water with absorbent paper, Then treat with 0.1% mercuric chloride solution for 8-10 minutes, rinse with sterile water for 6-7 times, blot dry with sterilized filter paper, and inoculate in improved B5 medium.
[0023] Effects of Explants on the Survival Rate and Induc...
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The invention discloses a tissue culture method for Limonium aureum. The method comprises the following steps: sterilizing an inflorescence of Limonium aureum; inoculating the sterilized inflorescence onto an inducing culture medium having a formula of B5+KT0.2-0.8mg / L+NAA0.05-0.1mg / L+2,4-D0.02-0.08mg / L, and inducing for 15-20 days to obtain Limonium aureum calluses; taking out the Limonium aureum calluses, inoculating onto a multiplying culture medium having a formula of B5+KT0.5-0.8mg / L+NAA0.05-0.1mg / L, and culturing for 30 days to obtain Limonium aureum adventitious buds; and inoculating the adventitious buds onto a rooting culture medium having a formula of B5+IBA0.1mg / L, and performing rooting culture for 7 days to obtain sterile seedlings. According to the invention, propagation based on the tissue culture method is not restricted by natural conditions; the inductivity and rooting rate are high; mass propagation can be performed continuously; and a large number of young seedlings can be obtained in a short time, thereby solving the problem of little seed source during culture.
Description
technical field [0001] The invention belongs to the field of plant biotechnology, and relates to a method for promoting plant regeneration through tissue culture technology, in particular to a method for tissue culture of pine pine. Background technique [0002] Limonium au-reum, also known as golden limonium and limonium limonium, is a perennial herb. The yellow-flowered rock pine belongs to the genus Limonium genus of Orchidaceae. It is one of the few wild flowers in the arid desert area. It has bright colors and a long flowering period. The root is a taproot system, thick and smooth, the whole plant is smooth, the plant height is 20-50cm, extensive management, extremely drought-resistant, long flowering period, it is an important germplasm resource for enriching the ground cover plant varieties in the greening construction of northern cities, and it is also windproof and sand-fixing. fine plants. The yellow-flowered rocky pine is very resistant to drought and high tempe...
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IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/005A01H4/008
Inventor 刘思泱马怀林张永清白俊梅智磊崔海鹏李晶晶云娜王召明高秀梅田志来李德奎徐沁贞杨阳
Owner INNER MONGOLIA M GRASS ECOLOGY & ENVIROMENT GRP CO LTD