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A method of regulating melanocyte pigment synthesis based on LED technology

A technology of melanocytes and pigments, applied in the fields of biotechnology and phototherapy applications, to achieve the effect of inhibiting tyrosinase activity, inhibiting expression, and enhancing curative effect

Inactive Publication Date: 2020-11-13
AFFILIATED HUSN HOSPITAL OF FUDAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, there are few reports on the application of LED light modulation in pigmented diseases. The research on light modulation is mainly aimed at fibroblasts, leukocytes, macrophages, mast cells, and keratinocytes. Therefore, based on the development and application of LED technology , and the status quo of research on the influence of LED light on skin pigmentation, the inventors of the present application intend to provide a new method for regulating the synthesis of melanocyte pigment, and further study the influence of LED light on the biological characteristics of melanocytes, so as to establish a A new model for the in vitro study of pigment disorders, as well as providing important support in the treatment and medical aesthetics of pigment disorders

Method used

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  • A method of regulating melanocyte pigment synthesis based on LED technology
  • A method of regulating melanocyte pigment synthesis based on LED technology
  • A method of regulating melanocyte pigment synthesis based on LED technology

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Experimental program
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Effect test

Embodiment 1

[0038] Embodiment 1, the experiment of regulating melanocyte pigment synthesis based on LED technology

[0039] 1. Isolation and culture of normal human primary melanocytes

[0040] Prepare normal human primary melanocyte single cell suspension: soak the obtained isolated normal human skin tissue in 75% ethanol solution for 30 sec, then wash with PBS for 3-4 times until there is no blood, and remove the subcutaneous tissue under aseptic conditions , blood vessels and part of the dermis were trimmed into thin strips of 1 mm × 5 mm, moved to 0.5% dispase and digested at 4°C for 16 hours, the serum was terminated for digestion, the epidermis and dermis were separated, the dermis was removed, and the obtained epidermis was placed in 0.25% trypsin-0.02% EDTA37 Incubate at ℃ for 10 minutes, tap the basal layer of the epidermis, blow it into a single cell suspension with a pipette; filter with a 200 μm sieve, centrifuge at 1500r / min for 7 minutes, discard the supernatant, add 5ml of ...

Embodiment 2

[0059] Embodiment 2 clinical experiment

[0060] Select a middle-aged female patient with a history of chloasma for 1 year, and give LED yellow light (585±5nm), 20J / cm2 , irradiated once a week, after 10 weeks of continuous intervention treatment, the chloasma on both cheeks of the patient was obviously improved, and a good curative effect was obtained, suggesting that the method of the present invention is helpful for formulating pigmentation diseases such as chloasma. intervention measures.

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Abstract

The invention belongs to the application field of biotechnology and phototherapy and specifically relates to a novel light emitting diode (LED) technology-based method for regulating melanocyte pigment synthesis. Through determined high-purity single spectrum LED light, melanocyte pigment synthesis is regulated, and proliferation and apoptosis of melanocyte are not affected. According to the method, LED yellow light with wavelength of 585+ / -5 nm, light intensity of 18 mw / cm<2> and energy range of 20J / cm<2> is adopted to continuously irradiate melanocyte. Then, tyrosinase activity of melanocyte can be inhibited, melanosome maturation is inhibited to reduce mature pigment granules in the melanocyte III-IV stage, expression of genes and protein related to melanogenesis is inhibited, melanin content is reduced, and proliferation and apoptosis of melanocyte are not affected. The method is safe and effective, can be used for preparation of an in vitro study model of melanocyte, is helpful for making intervening measures for treatment and adjuvant treatment of pigment disorders, and is used for making intervening measures for non-ablative medical cosmetology.

Description

technical field [0001] The invention belongs to the field of biotechnology and phototherapy applications, and specifically relates to a new method for regulating melanocyte pigment synthesis based on light-emitting diode (LED) technology. Pigment synthesis without affecting the proliferation and apoptosis of melanocytes. The method can provide a new model for in vitro research on pigmentation disorders, and can provide new ideas for clinical treatment of pigmentation disorders. Background technique [0002] The prior art discloses that melanocytes are a type of dendritic-like cells that reside at the dermal-epidermal junction of the skin, and that the melanin produced and transported to the surrounding keratinocytes determines the color of the skin and hair. Studies have reported that abnormal melanocytes can lead to a wide variety of pigmented skin diseases, hypopigmentation such as vitiligo, non-pigmented nevus, mottled disease, etc., and hyperpigmentation such as chloasm...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N13/00C12N5/071A61N5/06
Inventor 项蕾红陈力姜敏赵珏敏张成峰
Owner AFFILIATED HUSN HOSPITAL OF FUDAN UNIV