Color source primer transparent circle method for detecting lysozyme activity in daily chemical product

Abstract

The invention relates to a color source primer transparent circle method for detecting lysozyme activity in a daily chemical product. The method comprises the following steps: by virtue of hydrolysis of lysozyme, forming a transparent ring in a solid culture medium comprising a color source primer, wherein the color source primer is micrococcus lysodeikticus marked by an active dye bright-red, and in a proper concentration range, the vitality of lysozyme and the diameter of the transparent ring are of a linear relationship; drawing a standard gradient curve by taking lysozyme vitality unit as vertical coordinates and the diameter of the transparent ring as horizontal ordinates; and comparing the standard gradient curve with the standard gradient curved of a standard sample according to the diameter of the transparent ring of the sample, and calculating the vitality unit of the lysozyme in the sample. The method provided by the invention can be used for qualitatively and quantitatively detect the lysozyme, can exclude inference caused by factors such as colloids, and meanwhile, further can reflect the splitting specificity of the lysozyme to the bacterial cell walls. The transparent ring cannot be formed as other antibacterial components cannot decompose the bacterial cell walls dyed by the color source primer, so that the method is intuitive, fast, sensitive, simple and correct.

Description

technical field [0001] The invention relates to a method for detecting enzyme activity, in particular to a method for detecting lysozyme activity in daily chemical products. Background technique [0002] A wide variety of daily-use chemicals, with the development of science and technology, the production is increasingly sophisticated, in the selection of raw materials, the use of natural raw materials is becoming more and more popular. At the same time, in the research and development of functions, it is increasingly dependent on the development of molecular biology, bioengineering, skin physiology and medicine. Enzyme preparations are more and more concerned by the daily chemical industry because of their safety, high efficiency and specificity. Biological enzyme compound preparations with high stability and high efficiency are constantly opening up a new era in the field of daily chemicals. [0003] Biological enzyme is a catalyst produced by organisms, and its chemical ...

AI Technical Summary

Problems solved by technology

[0010] The technical problem to be solved by the present invention is to provide a color source substrate transparent circle method for detecting lysozyme activity for the above-mentioned prior art and for the characteristics of complex ingredients of daily chemical products. The advantages of reliable results, easier and faster detection

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