In-vitro flowering technology of hybrid Chinese orchid
A test-tube flowering technology, which is applied in horticulture, botany equipment and methods, gardening tools/equipment, etc., can solve the problems of huge segregation of traits of hybrid offspring, high cost of screening new varieties, high cost of cultivation, etc. , to achieve the effect of strong basis for selecting excellent germplasm, saving time and economic costs, and improving breeding efficiency
Active Publication Date: 2017-03-29
FUJIAN AGRI & FORESTRY UNIV
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Problems solved by technology
The cultivation and appreciation of Chinese orchids is an integral part of Chinese traditional culture, and the market demand is huge. However, due to the long-term cultivation of Chinese orchids, the selection of new varieties takes a long time and the cost is high. After the orchid seedlings of the hybrid offspring are cultured out of the bottle, it generally takes at least 2 years. -It takes 3 years of cultivation and care to see flowering, and the segregation of hybrid offspring has huge differences in traits. It usually takes 7-10 years, sometimes even longer, from the determined flowering product to the multiplication and sales, and the period is wasted Therefore, shortening the flowering period to screen and eliminate early hybrid seedlings has become a hot spot in the research of hybrid orchids in recent years.
There is no report on the test-tube flower-promoting technology of hybrid spring orchid rhizomes and all flowers are normal
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Embodiment 1
[0025] Material collection method: Select the rhizomes that grow robustly after germination and propagation of the hybrid seeds of Huangmei♀×Huanghe♂ for two subcultures as flower-promoting materials for inoculation;
[0026] Culture medium: MS basic medium was prepared with the formula in "Wood Plant Tissue Culture and Its Application" edited by Chen Zhenghua, Beijing Higher Education Press, 1986.
[0027] Medium preparation: the basic medium is MS, and Su (white sugar) 35 g·L -1 and Ag (agar strip) 7 g L -1 Heat to dissolve, add activated carbon Ac to dissolve in cold water first, add growth regulators NAA and 6-BA, use a pH meter with a temperature test range of 0-80°C and 1mol L -1 NaoH, 1mol L -1 HCL was used to adjust the pH to 5.7, and poured into 650 mL tissue culture glass bottles (100 mL per bottle), with a medium thickness of 1.5 cm.
[0028] Flower bud induction medium: MS + NAA 0.5 mg L -1 +6-BA 8.0 mg·L -1 + Su 35 g L -1 +Ac 1.5 g·L -1 + Ag 7 g L -1...
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The invention provides a method for direct in-vitro flowering of a hybrid Chinese orchid without a cultivation phase, thereby solving the problems that a hybrid Chinese orchid in the prior art is long in flowering life and the flowering type of the hybrid Chinese orchid cannot be determined before flowering are solved. Therefore, unnecessary labor and material resources are saved, and rhizomes of the hybrid Chinese orchid with the best flowering type are directly selected. According to the technology, rhizomes of the hybrid Chinese orchid are selected for induction culture, the rhizomes begin to bud at the eleventh day, macroscopic flower buds appear after two months, and the whole full-bloom stage appears after another two and half months. The normal flowering rate can reach 11.5%, and floral shapes are integrated.
Description
technical field [0001] The invention relates to a method for flowering hybrid spring orchids in test tubes, in particular to a method for inducing flower buds to form flowers from rhizomes of hybrid spring orchids. Background technique [0002] Chunlan ( Cymbidium goeringii ) is one of the earliest orchids cultivated in my country. Its leaves are generally thicker in texture and mostly green in color. It is famous for its good texture, beautiful petal shape, and mellow fragrance; Fu, which shows that the cultivation of Chunlan was relatively common at that time. The cultivation and appreciation of Chinese orchids is an integral part of Chinese traditional culture, and the market demand is huge. However, due to the long-term cultivation of Chinese orchids, the selection of new varieties takes a long time and the cost is high. After the orchid seedlings of the hybrid offspring are cultured out of the bottle, it generally takes at least 2 years. -It takes 3 years of cultivatio...
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IPC IPC(8): A01H4/00
CPCA01H4/008
Inventor 彭东辉漆子钰兰思仁吴沙沙翟俊文艾叶江鸣涛陈俊晖徐江宇金红
Owner FUJIAN AGRI & FORESTRY UNIV