Cultivation method for low-temperature-resistant nitrifying bacteria and application of low-temperature-resistant nitrifying bacteria in waste water treatment
A cultivation method and technology for nitrifying bacteria, applied in chemical instruments and methods, biological water/sewage treatment, water/sludge/sewage treatment, etc., can solve the problems of substandard sewage treatment, unstable operation at low temperature in winter, etc. The effect of the rapid degradation of substrates by the bacteria, the wide adaptation range of the bacteria, and the enhanced ability of the bacteria to adapt to temperature changes
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Embodiment 1
[0023] According to the inoculation amount of 20% (sludge 30-minute sedimentation ratio), insert the activated sludge rich in nitrifying bacteria from a sewage treatment plant into the nitrifying bacteria culture reactor. The water quality of the industrial ammonia-containing wastewater used is: the concentration of ammonia nitrogen is 100 ~150mg / L, COD 50~100mg / L, pH 7~8. When replacing the culture medium in each batch, add nitrifying bacteria growth promoter I at the same time, and dosing according to the concentration of the promoter in the culture system is 20mg / L. The cultivation process adopts the method of normal temperature and low temperature cultivation alternately. First, one batch is cultivated at normal temperature at a temperature of 25°C, and then one batch is cultured at a low temperature at a temperature of 15°C, and then transferred to normal temperature cultivation. Alternately carry out 10 times with low temperature culture, end one cycle of culture process...
Embodiment 2
[0025] According to the inoculation amount of 25% (sludge 30-minute sedimentation ratio), insert the activated sludge rich in nitrifying bacteria from a sewage treatment plant into the nitrifying bacteria culture reactor. The water quality of the industrial ammonia-containing wastewater used is: the ammonia nitrogen concentration is 200 ~250mg / L, COD 200~500mg / L, pH 7.5~8.3. When replacing the culture medium in each batch, add nitrifying bacteria growth promoter II at the same time, and dosing according to the concentration of the promoter in the culture system is 25mg / L. The cultivation process adopts the method of normal temperature and low temperature cultivation alternately. First, 2 batches are cultivated at room temperature at a temperature of 30°C, and then 2 batches are cultured at a low temperature at a temperature of 12°C, and then transferred to normal temperature cultivation. Alternately carry out 6 times with low temperature culture, end one cycle of culture proce...
Embodiment 3
[0027] According to the inoculum amount of 30% (sludge 30-minute sedimentation ratio), insert the activated sludge rich in nitrifying bacteria from a sewage treatment plant into the nitrifying bacteria culture reactor. The water quality of the industrial ammonia-containing wastewater used is: the ammonia nitrogen concentration is 300 ~400mg / L, COD 500~600mg / L, pH 7.5~8.5. Add nitrifying bacteria growth promoter III at the same time of replacing the culture medium in each batch, and add according to the concentration of the promoter in the culture system 30mg / L. The cultivation process adopts the method of normal temperature and low temperature cultivation alternately. First, 3 batches are cultivated at normal temperature at a temperature of 35°C, and then 4 batches are cultured at a low temperature at a temperature of 15°C, and then transferred to normal temperature cultivation. Alternately carry out 4 times with low-temperature cultivation, end one cycle of cultivation proces...
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