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Capillary-assisted vitrification method and device

A vitrification, capillary technology, used in biochemical cleaning devices, biochemical equipment and methods, enzymology/microbiology devices, etc., can solve problems such as degradation, dry storage and long-term storage limitations

Active Publication Date: 2019-12-10
SOMNIO GLOBAL HLDG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Dry preservation suffers major limitations in long-term storage due to the degradation of biomaterials due to the cumulative chemical stress encountered when vitrification solutions are concentrated in the extracellular space

Method used

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  • Capillary-assisted vitrification method and device
  • Capillary-assisted vitrification method and device
  • Capillary-assisted vitrification method and device

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0100] 1. Experiments Using Chinese Hamster Ovary (CHO) Cells

[0101] Cell culture: Chinese hamster ovary (CHO) cells were obtained from American Type Culture Collection (ATCC, Manassas, VA) and supplemented with 10% fetal bovine serum (Atlanta Biologicals, Norcross, GA) and 2% penicillin-streptomycin (10 U / mL penicillin G and 10 μg / mL streptomycin sulfate, Invitrogen, Carlsbad, CA) in Dulbecco's Modified Eagle Medium (DMEM) (Invitrogen, Carlsbad, CA). Cultures were maintained at 37 °C in 25-cm 2 T-flask (Corning Incorporated, NY) and with 10% CO 2 –90% air balance. After the cells were grown to the desired confluency (confluency) of 70%, the cells were trypsinized, pelleted and redispersed to yield 1×10 in DMEM. 5 cells / mL concentration.

[0102] Device Assembly: A PDMS cast was created with dimensions of 0.25 inches x 0.38 inches, with a central circular lumen (0.05 inches), and a polypropylene membrane with a pore size of 5 μm and a thickness of 100 μm (from Sterlitech...

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Abstract

Apparatus and methods for non-cryogenic vitrification of biological material are disclosed, comprising the step of providing one or more capillary channels, the first opening of which is operable to communicate with a vitrification agent made of biological material and containing Moisture in contact with the vitrified mixture. The capillary absorbs and transports moisture by capillary action to the second opening, and the moisture then evaporates into the surrounding low humidity atmosphere until the vitrified mixture enters a vitrified state.

Description

[0001] Cross References to Related Applications [0002] This application is dependent on and claims priority from US Provisional Application No.: 62 / 009,562, filed June 9, 2014, which is hereby incorporated by reference in its entirety. technical field [0003] One aspect of the present invention relates to non-low temperature vitrification of biological materials, in particular to biological materials vitrified in a vitrification medium (medium) by capillary assisted rapid drying. Background technique [0004] Vitrification is a process of direct transition from a liquid state to an amorphous glass state and is commonly used to preserve biological materials by cooling them to low temperatures at high cooling rates. At low temperatures, vitrification techniques avoid the damaging effects of ice crystals known to form during conventional cryopreservation using slower cooling rates. However, to avoid ice nucleation during cooling, extremely high and potentially toxic concent...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12M1/00A01N1/00
CPCC12M47/14A01N1/0263A01N1/0242A01N1/0278A01N1/0252A01N1/0284
Inventor P·S·莫汉蒂N·查克拉伯蒂
Owner SOMNIO GLOBAL HLDG