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Enzyme linked immunosorbent assay kit used for detecting trifluralin, and detection method thereof

An enzyme-linked immunosorbent assay technology and a detection method are applied in the field of enzyme-linked immunosorbent assay kits to achieve the effects of sensitive detection and simple operation.

Inactive Publication Date: 2017-05-03
ZHENJIANG XIANCHUANG BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Ineffective against adult weeds

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0020] Preparation of trifluralin protein conjugates:

[0021] The carboxyl-containing trifluralin hapten derivative was obtained by the succinic anhydride method, and then 0.05 mmol was mixed with the carrier protein BSA in a 10:1 binding ratio in 0.05 mol / L carbonate buffer solution (CBS) of pH 9.6, Then add 0.15 mmol carbodiimide, stir and react at room temperature for 24 h, and finally dialyze in 0.2 mol / L PBS buffer solution with pH 7.6 for two days to remove unreacted hapten, and dilute the obtained protein conjugate solution in - Store at 20°C for later use.

[0022] Preparation of trifluralin antibody:

[0023] Select healthy adult purebred BALA / C mice, take 50 μg of immune antigen prepared by coupling with protein and mix with the same amount of complete Freund's adjuvant, and use intraperitoneal injection for initial immunization, and then use the same dose of immune antigen plus the same amount every 3 weeks Incomplete Freund's adjuvant was used for the second and...

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Abstract

The invention discloses an enzyme linked immunosorbent assay kit used for detecting trifluralin, and a detection method thereof. The enzyme linked immunosorbent assay kit used for detecting trifluralin is sensitive, accurate, and rapid in detection, is simple in operation, and is high in specificity, and is suitable for detection of a large amount of samples. The enzyme linked immunosorbent assay kit comprises an ELISA plate coated with trifluralin antigen, a trifluralin standard substance, a trifluralin antibody working solution, a trifluralin enzyme-labelled second antibody working solution, a substrate solution A, a substrate solution B, a stopping solution, a condensed diluent, and a condensed washing liquid. The enzyme linked immunosorbent assay kit used for detecting trifluralin is invented based on solid phase indirect competitive enzyme-linked immune response principles. According to the detection method, an extracted sample, the trifluralin enzyme-labelled second antibody working solution, and the trifluralin antibody working solution are introduced into corresponding ELISA plate micropores, incubation is carried out for a certain period of time, the substrate solution A and the substrate solution B are added for washing, a color developing agent becomes blue under enzyme action, the stopping solution is added, and the color is changed from blue to yellow. The content of trifluralin in the sample is inversely proportional to the shade of the color, and the detection method can be applied to detection of trifluralin residual quantity in crops directly.

Description

technical field [0001] The invention relates to the technical field of veterinary drug residue detection, in particular to an ELISA kit for detecting trifluralin in rice. Background technique [0002] Trifluralin is an excellent dry field herbicide. It is mainly used in cotton, soybean, peanut, sunflower, cabbage, pepper, beans, orchard, etc. It can also be used in wheat field and upland rice field to control annual grasses and multi-growing weeds and some broad-leaved weeds propagated by seeds , such as barnyardgrass, foxtail, crabgrass, kanmai, cricket grass, daughter of a thousand golds, bluegrass, brome, wild oats, Buzz grass, quinoa, amaranth, corngrass, chickweed, Kochia, purslane, etc. . Poor or basically ineffective control of perennial root weeds such as Solanum nigrum, Cocklebur, Abutilon and so on. Ineffective against adult weeds. Sensitive crops such as sorghum and millet cannot be used; sugar beets, tomatoes, potatoes, cucumbers, etc. are not highly resistan...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/535
CPCG01N33/535
Inventor 洪霞杜霞刘静
Owner ZHENJIANG XIANCHUANG BIOTECH CO LTD