Method for breeding cabbage type rape radish cytoplasmic male sterility three-line

Abstract

The invention discloses a method for breeding a cabbage type rape radish cytoplasmic male sterility three-line. The method comprises the steps of adopting cabbage type rape radish cytoplasmic sterile line (Ogu CMS) coeno-species as a female parent, adopting fertility as a marker character, adopting a superior line as a male parent, utilizing a heredity principle for combining the fertility marking and backcross breeding, considering both selection of an economical character and a quality character, directionally guiding a restoring gene of Ogu CMS into an excellent cabbage type rape parent, breeding to obtain a new cabbage type rape Ogu CMS restore line, and acquiring new mating sterile and maintainer lines at the same time. Compared with a method for utilizing cytoplasmic male sterile lines hybridization opposite-measurement or molecular marker assistant selection for breeding the restore line, the method for breeding the cabbage type rape radish cytoplasmic male sterility three-line is accurate and high-efficient, can quickly create a series of new rape Ogu mating three-line resources, and is wide in application prospect and remarkable in benefit.

Description

technical field [0001] The invention belongs to the technical field of breeding methods, and in particular relates to a method for breeding three cytoplasmic male sterile lines of Brassica napus radish. Background technique [0002] Among the many ways and methods of heterosis utilization in rapeseed, the most common and effective way is to create hybrids represented by cytoplasmic sterility (CMS), nuclear sterility (GMS) and chemically induced sterility (CHA). At present, there are about 20 rapeseed CMSs reported at home and abroad. The most commonly used ones in production are Polima CMS, Shaanxi 2A CMS, and radish cytoplasmic CMS (Ogu CMS). CMS is mainly popularized and applied in Europe and the United States. Although there are related researches in my country, it started late and its application lags behind. [0003] Ogu CMS is a sterile material found in radish germplasm resources by Japanese agronomist Ogura in 1968. French Bannerot (1974) introduced the nucleus of Br...

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to provide a method for breeding three lines of cytoplasmic male sterility in Brassica napus radish, aiming to solve the problem that the quality traits and agronomic traits of the three lines of Ogu CMS in Brassica napus are poor, and there are few restorer lines with different genetic backgrounds. There is a lack of stable three-lines, and there are some difficulties in the matching and application of hybrids.

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