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Cultivation method and application of space Lactobacillus plantarum lct-lp1

A technology of LCT-LP1 and Lactobacillus plantarum, which is applied in the direction of microbial-based methods, biochemical equipment and methods, microbial measurement/inspection, etc., and can solve long-term on-orbit time, flight safety threats, accelerated degradation and wear of aerospace devices, etc. problem, to achieve the effect of enhanced corrosion ability

Active Publication Date: 2019-06-25
FULLARTON BIOENG TECH BEIJING CO LTD
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The large number of corrosive microorganisms in the space airtight cabin will gradually corrode various aerospace materials and accelerate the degradation and wear of aerospace devices, which may cause equipment failure in the space station and affect the long-term normal operation of the spacecraft and its service life in orbit. , and even pose a great threat to flight safety
The service conditions of the spacecraft are harsh, the time on orbit is long, and the maintenance conditions are relatively insufficient. Once the microbial corrosion equipment fails, the loss is immeasurable
The on-orbit experience of foreign manned spacecraft and the preliminary results of domestic ground research show that microorganisms will seriously threaten the safety of aerospace equipment

Method used

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  • Cultivation method and application of space Lactobacillus plantarum lct-lp1
  • Cultivation method and application of space Lactobacillus plantarum lct-lp1
  • Cultivation method and application of space Lactobacillus plantarum lct-lp1

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Example 1. Obtaining Lactobacillus plantarum LCT-LP1

[0038] 1. Bacteria collection

[0039] Microorganisms were collected inside the return cabin of the "Shenzhou 10" spacecraft. The bacterial culture medium was selected for preliminary screening of the samples, and then single colonies with different colony morphologies were selected for streaking culture, and after purification to a single strain, it was named as strain LCT -LP1.

[0040] 2. Morphological identification

[0041] The strain LCT-LP1 obtained in step 1 was subjected to Gram staining, and the results showed that the strain LCT-LP1 was Gram-positive, Brevibacterium. A single colony of strain LCT-LP1 was inoculated on the plate to observe the growth morphology of the colony, and the microscopic morphology was observed by scanning electron microscope. Scanning electron microscopy observes that the bacteria are arranged in a short rod or spherical shape (see figure 1 ). The colonies on soft agar are dense and wh...

Embodiment 2

[0051] Example 2. Biofilm formation experiment of Lactobacillus plantarum LCT-LP1

[0052] 1. Under aseptic conditions, add 50 mL of sterilized Lactobacillus plantarum culture medium to each 100 mL blue cap bottle, and then access Lactobacillus plantarum LCT-LP1 (the initial stage of Lactobacillus plantarum LCT-LP1 in the culture system). The concentration is 10 4 Pieces / m1), cultured at 37°C for 2 days.

[0053] 2. Transfer the Lactobacillus plantarum LCT-LP1 culture obtained in step 1 to a 96-well plate and culture it at 37°C for 24 hours, observe the biofilm formation in the well plate, and then select the colonies in the well plate to transfer to Cultivation and comparison were performed in a glass test tube, and the stable formation of the biofilm was observed and compared again.

[0054] Set up three groups of treatments: in the first group, the culture conditions of step 1 and step 2 are the culture conditions of the space flight group; in the second group, the culture condit...

Embodiment 3

[0056] Example 3 Corrosion test of Lactobacillus plantarum LCT-LP1

[0057] 1. Preparation of metal test piece

[0058] 1. Select the metal material TC4 titanium alloy (Ti-6A1-4V, (α+β) alloy) and 304 stainless steel (OCr18Ni9), and process them into a cylindrical sample with a cross-sectional diameter of 10mm and a length of 4mm. 400#, 800#, 1200#, 2000# metallographic sandpaper polishing.

[0059] 2. Polish the 304 stainless steel sample polished in step 1 with diamond abrasive paste.

[0060] 3. Polish the TC4 titanium alloy sample polished in step 1 with iron oxide and chromium oxide solution, remove grease on the surface of the sample with acetone, and then corrode with 10% (mass fraction) sodium hydroxide aqueous solution at 50-60℃1 -2min to remove the surface oxide layer.

[0061] 4. After the treatments of step 2 and step 3, rinse the 304 stainless steel sample and the TC4 titanium alloy sample with tap water, then immerse in 30% (volume fraction) nitric acid aqueous solution ...

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Abstract

The invention discloses a culture method of space lactobacillus plantarum LCT-LP1 and application. The method for culturing lactobacillus plantarum LCT-LP1 comprises the following step of culturing lactobacillus plantarum by adopting a lactobacillus plantarum culture medium, wherein the preservation number of lactobacillus plantarum LCT-LP1 is CGMCC (China General Microbiological Culture Collection Center) NO.12578. According to the culture method disclosed by the invention, researches on mechanisms and prevention and control measures of corrosion to aerospace equipment, caused by the fact that microorganisms exist in an aerospace closed cockpit for a long period, are facilitated, and understanding on microorganism risks in a manned spacecraft is easy to deepen and related technical storage is easy to enhance; and more effective microorganism comprehensive control measures of the manned spacecraft are easy to explore, and technical supports are provided for development and operation of the manned spacecraft.

Description

Technical field [0001] The invention relates to the field of microorganisms and biotechnology, in particular to a method and application for culturing space Lactobacillus plantarum LCT-LP1. Background technique [0002] With the increasing frequency of human space exploration activities, cosmic space has become an important field of human activities. There are specific microbial communities in the space capsule. Although every item has been strictly disinfected, microorganisms will still enter space with the astronaut’s body, human secretions, aviation components, etc., and form specific microbial communities in the airtight compartment of the spacecraft, including harmless microorganisms and some Pathogenic and corrosive microorganisms. 234 kinds of microorganisms have been detected in the space station in the past ten years of operation of the Mir. Special environmental factors in the airtight capsule, including microgravity, weak magnetic field, and particle radiation, caus...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12Q1/02C12R1/25
CPCC12N1/20C12Q1/02
Inventor 郝红炜黄兵
Owner FULLARTON BIOENG TECH BEIJING CO LTD
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