Method for detecting influence of smoke total particulate matter on cell inflammatory effect factor

Abstract

The invention discloses a method for detecting influence of smoke total particulate matter on a cell inflammatory effect factor. The method comprises the following steps of: (1) preprocessing of a tested substance; (2) preparation of human lung fibroblast single-cell suspension; (3) calculation of cell concentration of the human lung fibroblast single-cell suspension; (4) cell inoculation of the human lung fibroblast single-cell suspension; (5) grouping of the tested substance; (6) setting of a detection dosage of the tested substance; (7) preparation of a tested substance culture product; (8) incubation of the tested substance; (9) collection of tested substance incubation products; (10) preparation of standard solution; (11) adding of a sample to be detected; (12) adding of an antibody; (13) adding of binding solution; (14) color development; (15) measurement of a light absorption value; (16) measurement of a cell factor secretion volume. According to the method disclosed by the invention, influence of the cigarette smoke total particulate matter on secretion of the cell inflammatory effect factor can be accurately and effectively detected.

Description

Technical field [0001] The invention belongs to the technical field of tobacco product biological effect evaluation, and particularly relates to a method for detecting the influence of total smoke particles on cell inflammatory effect factors. Background technique [0002] As people pay more and more attention to health, higher requirements are put forward on tobacco products, not only to have a better taste, but also to reduce the bad feelings of the human body as much as possible. Cigarette product R&D personnel combine different components in different proportions at the early stage of e-liquid product formulation design to form primary formulas with different styles, and then combine chemical evaluation and sensory evaluation to continuously screen and adjust the formula to form the final product formula. However, there are a large number of primary formulations, and all sensory evaluations are time-consuming and labor-intensive. Sensory evaluation focuses on the selection of...

AI Technical Summary

Problems solved by technology

However, there are a large number of primary formulas, and sensory evaluation of all of them is time-consuming and labor-intensive, and the sensory evaluation focuses on screening the style and taste of the product. How to use biological test indicators to screen the primary formula of the product to obtain the effect of reducing the adverse feelings of the human body? The product formula is still in the exploratory stage, and there is no uniform standard method

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