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Method for collecting syzygium buxifolium tissue culture initial bud

An acquisition method, the technology of the red nan group, applied in the field of initial bud acquisition of red nan tissue culture, can solve the problems of explant disinfection difficulty, low initial bud occurrence rate, poor bud activity, etc., to promote rapid development, initial The effect of high bud formation rate and fast elongation of sprouts

Inactive Publication Date: 2017-05-17
唐春艳 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The present invention aims at the technical problems such as difficulty in disinfection of explants, browning, low incidence of initial buds, and poor activity of sprouts in the existing red nan tissue culture, and provides a method for preparing aseptic explants of red nan and inducing initial buds

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] In a sunny climate for at least 3 consecutive days, collect healthy young shoots of the year of red nan as explants. After the explants were rinsed with sterile water, they were soaked in a broad-spectrum fungicide with a volume concentration of 0.1% for 30 min, and the explants were taken out, soaked in 70% alcohol for 1 min, and then moved into Soak in sodium hypochlorite solution with a volume concentration of 5% for 20 minutes, take it out and put it in a mixture of 10% mercuric chloride and 2 drops of Tween, stir and soak for 30 minutes, and finally wash it with sterile water for 3 times, each time for 5 minutes. min; cut the explants into stem segments with at least one axillary bud and a length of 1.5-2.0 cm, and put them in containers according to the degree of lignification of the stem segments, and put them in containers for later use; inoculate the explants obtained after disinfection in the induction medium Medium; the induction medium is: modified 1 / 2MS med...

Embodiment 2

[0023] In a sunny climate for at least 3 consecutive days, the young shoots of the year that grew robustly in red nan were collected as explants. After the explants were rinsed with sterile water, they were soaked in a broad-spectrum fungicide with a volume concentration of 0.2% for 25 min, and the explants were taken out and soaked in 70% alcohol for 2 min, and then moved into Soak in sodium hypochlorite solution with a volume concentration of 6% for 15 minutes, take it out and put it in a mixture of 15% mercuric chloride and 4 drops of Tween, stir and soak for 25 minutes, and finally wash it with sterile water for 3 times, each time for 5 minutes. min; cut the explants into stem segments with at least one axillary bud and a length of 2.0-2.5 cm, and put them in containers according to the degree of lignification of the stem segments, and put them in containers for later use; inoculate the explants obtained after disinfection in the induction medium Medium; the induction medi...

Embodiment 3

[0026] In a sunny climate for at least 3 consecutive days, the young shoots of the year that grew robustly in red nan were collected as explants. After the explants were rinsed with sterile water, they were soaked in a broad-spectrum fungicide with a volume concentration of 0.3% for 20 min, and the explants were taken out and soaked in 70% alcohol for 2 min, and then moved into Soak in sodium hypochlorite solution with a volume concentration of 8% for 10 minutes, take it out and put it in a mixture of 25% mercuric chloride and 5 drops of Tween, stir and soak for 20 minutes, and finally wash it with sterile water 4 times, each time for 3 minutes. min; cut the explants into 2.5-3cm long stem segments with at least one axillary bud, classify them into containers according to the degree of lignification of the stem segments, and put them in containers for later use; inoculate the explants obtained after disinfection into the induction medium ; The induction medium is: modified 1 / 2...

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Abstract

The invention discloses a method for collecting a syzygium buxifolium tissue culture initial bud. The method comprises the procedures of explant preparation, explant disinfection treatment and initial bud induction, and comprises the following specific steps: collecting a syzygium buxifolium annual shoot to serve as an explant; disinfecting the explant; inoculating the obtained sterile explant into an initial bud induction culture medium; culturing in a proper environment for 20 to 30 days to obtain a syzygium buxifolium initial bud which grows quickly and has high vitality. The annual syzygium buxifolium shoot is selected as a tissue culture propagation material, a disinfection treatment way of using a plurality of agents with certain concentration and disinfection time is adopted, so that the sterile explant collection rate is over 90 percent; an optimized induction culture medium and an optimized culture way are adopted, so that browning of the explant is reduced, the initial bud generation rate is high, and the bud grows quickly and has high vitality; the method has good economical benefit and social benefit.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture, and in particular relates to a method for obtaining initial buds of red nan tissue culture. Background technique [0002] Red Nan (Syzygium buxifolium Hook. et Arn.), belonging to Myrtaceae, is a shrub or small tree, with ridged shoots and dark brown after drying. Leaf blade leathery, broadly elliptic to elliptic, sometimes broadly obovate, apex round or blunt, sometimes with blunt apex, base broadly cuneate or blunt, upper part dark brown after drying, matte, lower part light-colored, with glandular spots, Lateral veins numerous and dense, inconspicuous above, slightly protruding below; styles equal to stamens. Fruit spherical, 5-7 mm in diameter. The flowering period is June-August. Produced in Anhui, Zhejiang, Taiwan, Fujian, Jiangxi, Hunan, Guangdong, Guangxi, Guizhou and other provinces and regions in China. Born in low mountain forests or shrubs. Red Nan likes light, is al...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/00A01H4/001
Inventor 唐春艳陈素云
Owner 唐春艳
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