Low-serum cell culture medium with universal adaptability and preparation method thereof
An adaptable, low-serum technology, applied in the field of biochemistry, can solve the problems of aggravating the economic burden of enterprises and rising animal serum prices, and achieves the effect of small serum dosage, no reduction in cell viability, and cost reduction.
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[0031] Example one
[0032] The steps of preparing the low serum cell culture medium of the present invention are as follows:
[0033] (1) Prepare solution: mix 0.0035 parts by mass of biotin, 0.2 parts by mass of riboflavin, 0.7 parts by mass of vitamin B12, 0.0012 parts by mass of copper sulfate pentahydrate, 0.05 parts by mass of ferric nitrate nonahydrate, and 0.5 parts by mass of ferrous sulfate heptahydrate , 0.75 parts by mass zinc sulfate heptahydrate, 0.0003 parts by mass ammonium metavanadate, 0.00006 parts by mass manganese chloride, 0.003 parts by mass sodium selenite, 2 parts by mass ethanolamine, 1 part by mass linoleic acid, 0.08 parts by mass lipoic acid , 0.06 parts by mass of putrescine dihydrochloride and 0.3 parts by mass of thymidine are dissolved in 1000 parts by mass of ultrapure water to prepare a solution;
[0034] (2) Drying: Mix the solution obtained in step (1) with 3000 parts by mass of D-glucose and then dry;
[0035] (3) Ball mill: Take the following ma...
Example Embodiment
[0039] Example two
[0040] The difference between this embodiment and the first embodiment is that: in step (1), 0.002 parts by mass of biotin, 0.1 parts by mass of riboflavin, 0.2 parts by mass of vitamin B12, 0.0005 parts by mass of copper sulfate pentahydrate, and 0.02 parts by mass of nine Ferric nitrate water, 0.2 parts by mass ferrous sulfate heptahydrate, 0.5 parts by mass zinc sulfate heptahydrate, 0.0001 parts by mass ammonium metavanadate, 0.00002 parts by mass manganous chloride, 0.002 parts by mass sodium selenite, 1 part by mass ethanolamine, 3 parts by mass linoleic acid, 0.05 parts by mass lipoic acid, 0.05 parts by mass putrescine dihydrochloric acid and 0.1 parts by mass thymidine are dissolved in 940 parts by mass of ultrapure water to prepare a solution;
[0041] In step (2), the solution prepared in step (1) is mixed with 1000 parts by mass of D-glucose and dried;
[0042] In step (3), take the following mass parts and mix:
[0043]
[0044]
[0045] Then it is m...
Example Embodiment
[0046] Example three
[0047] The difference between this embodiment and the first embodiment is that in step (1), 0.004 parts by mass of biotin, 0.3 parts by mass of riboflavin, 0.8 parts by mass of vitamin B12, 0.0015 parts by mass of copper sulfate pentahydrate, and 0.06 parts by mass of nine Ferric nitrate water, 0.6 parts by mass ferrous sulfate heptahydrate, 1 part by mass zinc sulfate heptahydrate, 0.0005 parts by mass ammonium metavanadate, 0.0001 parts by mass manganous chloride, 0.007 parts by mass sodium selenite, 1.5 parts by mass ethanolamine, 5 parts by mass of linoleic acid, 0.12 parts by mass of lipoic acid, 0.1 parts by mass of putrescine dihydrochloric acid and 0.5 parts by mass of thymidine are dissolved in 1,060 parts by mass of ultrapure water to prepare a solution;
[0048] In step (2), the solution prepared in step (1) is mixed with 4,500 parts by mass of D-glucose and dried;
[0049] In step (3), take the following mass parts and mix:
[0050]
[0051]
[0052...
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