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A method for rapid propagation of high-quality seedlings of red torch turmeric

A technology of torch turmeric and seedlings, applied in the field of rapid propagation of high-quality seedlings of red torch turmeric, to achieve the effect of simple and affordable method, rapid propagation, and high application value

Active Publication Date: 2019-06-21
广东天卉生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no report on the propagation of red torch turmeric seedlings by tissue culture at home and abroad

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] 1. Acquisition of explants and induction of adventitious buds: During the growing season, select excellent strains of Curcuma hybrida 'Red Torch' that grow vigorously and have no damage by diseases and insect pests to mark, and dig underground bulbs at the end of November. After washing with tap water, soak it in 50% carbendazim wettable powder 600 times solution for 20 minutes, dry it and store it in a cold storage at 10°C for 2 months, then bury the bulb in a place where it was washed with tap water and then used for 50 % Carbendazim wettable powder 600 times liquid watered fine sand, put into artificial climate box to induce dormant buds, culture temperature 25 ℃, dark culture. The red torch tulip bulbs will grow 1 cm small buds after 20 days of cultivation, and the small buds are used as explants for tissue culture. Small buds were first soaked in 75% alcohol for 10 seconds, then sterilized with 0.1% mercuric chloride solution for 5 minutes, rinsed with sterile wate...

Embodiment 2

[0022] 1. Acquisition of explants and induction of adventitious buds: During the growing season, select excellent strains of Curcuma hybrida 'Red Torch' that grow vigorously and have no damage by diseases and insect pests to mark, and dig underground bulbs at the end of November. After washing with tap water, soak it with 50% carbendazim wettable powder 700 times solution for 25 minutes, dry it and put it in a cold storage at 12°C for 2.5 months, then bury the bulb in a 50- % Carbendazim wettable powder 700 times liquid watered fine sand, put into artificial climate box to induce dormant buds, culture temperature 28 ℃, dark culture. The red torch tulip bulbs will grow small buds of 1.5 cm after 18 days of cultivation, and the small buds are used as explants for tissue culture. Small buds were first soaked in 75% alcohol for 20 seconds, then sterilized with 0.1% mercury solution for 8 minutes, rinsed with sterile water for 5 times, and inoculated into adventitious bud induction...

Embodiment 3

[0027] 1. Acquisition of explants and induction of adventitious buds: During the growing season, select excellent strains of Curcuma hybrida 'Red Torch' that grow vigorously and have no damage by diseases and insect pests to mark, and dig underground bulbs at the end of November. After washing with tap water, soak it with 50% carbendazim wettable powder 800 times solution for 30 minutes, dry it and put it in a cold storage at 15°C for 3 months, then bury the bulb in the water and wash it with tap water before using it for 50 minutes. % Carbendazim wettable powder 800 times liquid watered fine sand, put into artificial climate box to induce dormant buds, culture temperature 30 ℃, dark culture. The red torch tulip bulbs will grow 2 cm small buds after 15 days of cultivation, and the small buds are used as explants for tissue culture. Small buds were first soaked in 75% alcohol for 30 seconds, then sterilized with 0.1% mercury liter solution for 10 minutes, rinsed with sterile wa...

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PUM

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Abstract

The invention discloses a rapid propagation method for red-torch curcuma petiolata high-quality seedlings. The method comprises the following steps: performing dormancy bud induction for red-torch curcuma petiolata bulbs to obtain buds serving as an explant, performing adventitious bud induction, subculture multiplication, rooting culture and test-tube seedling transplanting, selecting culture medium formulas and culture conditions suitable for various stages, so that the rapid propagation of the red-torch curcuma petiolata seedlings is realized, and the high-quality red-torch curcuma petiolata seedlings are propagated to satisfy the market demand. The method is simple, economic, feasible and high in application value; and a simple plant tissue culture device is needed for implementing the method.

Description

[0001] Technical field: [0002] The invention belongs to the field of plant tissue culture, and in particular relates to a method for rapid propagation of high-quality seedlings of red torch turmeric. [0003] Background technique: [0004] Red Torch Turmeric (Curcuma hybrida 'Red Torch') is a new crop variety introduced from abroad, domesticated and approved by Guangdong Province in recent years by South China Botanical Garden. It has long inflorescences, many bracts, bright flowers, long flowering period, and good ornamental value. It can be used as potted plants or cut flowers for viewing, and can also be used for landscaping. Once launched, the supply exceeds demand. The commonly used propagation method of Red Torch Curcuma is to divide into balls, but the number of reproduction is limited and it is easy to be infected by pathogens. At present, there is no report on the tissue culture seedling propagation of Red Torch Curcuma at home and abroad. [0005] Invention conten...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 曾宋君文颖婷郑枫吴坤林张建霞张新华段俊马国华
Owner 广东天卉生物科技有限公司
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