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Full-automatic high-efficiency western blot membrane washing system

A fully automatic membrane washing technology, which is applied in the field of medical devices, can solve the problems of high reagent consumption, high labor cost, and inconvenient operation, and achieve the effects of low reagent consumption, low labor cost, and easy operation

Active Publication Date: 2017-05-31
SHANGHAI CHANGHAI HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The object of the present invention is to provide an automatic membrane washing, automatic adding / pitting solution for the shortcomings of traditional operation, such as inconvenient operation, long time consumption, large reagent consumption and high labor cost. , Fully automatic and efficient western blot washing system with easy operation

Method used

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  • Full-automatic high-efficiency western blot membrane washing system
  • Full-automatic high-efficiency western blot membrane washing system
  • Full-automatic high-efficiency western blot membrane washing system

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] A fully automatic and high-efficiency western blot membrane washing system, comprising: an upper shell 1 of the membrane washing device, the upper shell 1 of the membrane washing device is hollow and table-shaped, and cooperates with the base 2 of the membrane washing device, on which a water pump device, electric motor Device, rocker arm device and transmission device; liftable liquid change device 3, liftable liquid change device 3 is connected to the water pump device, and placed close to the film washing box 4, the film washing box 4 is located on the shaking table 5, and the shaking table 5 is controlled by the rocking arm The device is driven, and the electric device drives the rocker device through the transmission device; the electric device and the water pump device are controlled by the control board 10, and the control board 10 controls the water pump device and the electric device respectively.

[0046] The upper part of the upper shell 1 of the membrane wash...

Embodiment 2

[0065] Before use, set the rocking time, rocking distance, lifting angle, water pump running time (including liquid injection time, liquid drainage time) and the number of washing membranes of the shaking table 5 through the touch screen of the control panel 10 . When in use, first take out the membrane washing box 4, put the PVDF membrane or NC membrane to be washed into the membrane washing chamber 42 of the membrane washing box 4, then fix the membrane washing box 4 in the membrane washing box slot 51 of the shaker 5, The limiting post 55 of the membrane washing box on the shaker 5 is matched with the limiting groove 44 of the washing liquid box on the membrane washing box 4 . The liquid exchange chamber 41 faces one end of the liquid exchange rod 31 .

[0066] After the device is started, the liquid replacement rod 31 can be lifted and lowered, and the second liquid injection port 313 extends into the liquid replacement chamber 41 of the film washing box 4. According to th...

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Abstract

The invention relates to a full-automatic high-efficiency western blot membrane washing system. An upper casing of the membrane washing device is in a hollow platform shape, and is matched with a base of the membrane washing device; a water pump device, an electric device, a rocking arm device and a transmission device are fixedly arranged on the base of the membrane washing device; a liftable liquid replacing device is connected with the water pump device, and is arranged near the membrane washing box; the membrane washing box is positioned on a rocking bed, and can be fetched and placed; the rocking bed is driven by the rocking arm device, and the rocking arm device is driven by the electric device through the transmission device; the electric device and the water pump device are controlled by a control panel, and the control panel is used for respectively controlling the water pump device and the electric device. The full-automatic high-efficiency western blot membrane washing system has the advantages that the operation is simple and convenient, the time consumption is short, the labor cost is low, the automatic effect is realized, and the efficiency is high.

Description

technical field [0001] The invention relates to the technical field of medical devices, in particular to a fully automatic high-efficiency western blot membrane washing system used in basic biomedical experiments. Background technique [0002] Western blot, Western blotting, is an experimental method commonly used in molecular biology, biochemistry and immunogenetics. The basic principle is to stain the cells or biological tissue samples treated by gel electrophoresis through specific antibodies, and obtain information about the expression of specific proteins in the analyzed cells or tissues by analyzing the staining position and staining depth. The steps of Western blot roughly include sample preparation, protein content quantification, SDS-PAGE electrophoresis, membrane transfer (PVDF membrane or NC membrane), blocking, membrane washing, antibody incubation, chemiluminescence and other steps, and the whole process takes a long time. Among them, washing the membrane is an...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B08B3/10B08B13/00B08B3/08
CPCB08B3/10B08B13/00B08B3/08
Inventor 伍国胜陈郑礼李海航朱邦晖朱世辉孙瑜夏照帆
Owner SHANGHAI CHANGHAI HOSPITAL