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Extracting method for L-alanine

An extraction method, alanine technology, applied in the field of fermentation, can solve the problems of wasting L-alanine, environmental pollution, etc., and achieve the effects of reducing environmental protection pressure, simplifying the extraction process, and reducing equipment investment

Active Publication Date: 2017-05-31
QINHUANGDAO HUAHENG BIOENG CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

After the production of L-alanine by fermentation method, the method of multiple crystallization is generally used to purify L-alanine. The crystallization waste liquid generally contains part of L-alanine. In addition, the organic matter (mainly L-alanine) in the crystallization waste liquid alanine, amino acids and peptides, etc.), the direct discharge of waste liquid will not only waste part of the unrecovered L-alanine, but also pollute the environment

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Take 500ml L of L-alanine fermented liquid that is heated to 53 DEG C and be 10% in the clean dry beaker, add 20 mg concentration to be 9% L-alanine fermented liquid in described L-alanine fermented liquid Add the polyaluminum chloride solution while stirring, add 10 mg of chitosan solution with a concentration of 0.8% after the addition is completed and stir for 5 minutes, add while stirring, add 10 mg of polyacrylamide with a concentration of 0.1% after the addition is completed and stir for 5 minutes The solution was stirred for 5 minutes after addition and allowed to stand for 10 minutes. Add 300 mg of activated carbon to the L-alanine fermentation broth after the flocculation and decolorize for 30 minutes;

[0033]The decolorized L-alanine fermentation liquid is stirred and precipitated, and the L-alanine fermentation liquid after the stirring and precipitation treatment is put into a vacuum drum filtration device for separation to obtain flocculation and clarifica...

Embodiment 2

[0036] Take 500 ml of L-alanine fermentation broth with a L-alanine content of 15% heated to 55°C in a clean and dry beaker, and add 20 mg of L-alanine fermentation broth with a concentration of 10% to the L-alanine fermentation broth. Add polyaluminum chloride solution while stirring, add 10 mg of chitosan solution with a concentration of 0.1% after stirring for 5 minutes, add 10 mg of polyacrylamide with a concentration of 0.1% after adding and stir for 5 minutes The solution was added and stirred for 5 minutes and allowed to stand for 10 minutes. 300 mg of activated carbon was added to the flocculated L-alanine fermentation broth for decolorization for 30 minutes;

[0037] The decolorized L-alanine fermentation liquid is stirred and precipitated, and the L-alanine fermentation liquid after the stirring and precipitation treatment is put into a vacuum drum filtration device for separation to obtain flocculation and clarification The pre-coating material in the vacuum drum f...

Embodiment 3

[0040] Take 500 ml of L-alanine fermentation broth with a L-alanine content of 20% heated to 57°C in a clean and dry beaker, and add 25 mg of L-alanine fermentation broth with a concentration of 11% to the L-alanine fermentation broth. Add polyaluminum chloride solution while stirring, add 10 mg of chitosan solution with a concentration of 1.1% after stirring for 5 minutes, add 10 mg of polyacrylamide with a concentration of 0.1% after adding and stir for 5 minutes The solution was added and stirred for 5 minutes and allowed to stand for 10 minutes. 300 mg of activated carbon was added to the flocculated L-alanine fermentation broth for decolorization for 30 minutes;

[0041] The decolorized L-alanine fermentation liquid is stirred and precipitated, and the L-alanine fermentation liquid after the stirring and precipitation treatment is put into a vacuum drum filtration device for separation to obtain flocculation and clarification The pre-coating material in the vacuum drum f...

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PUM

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Abstract

The invention discloses an extracting method for L-alanine. The method comprises the following steps: adding a first flocculating agent, a second flocculating agent and a third flocculating agent into L-alanine fermentation liquor to perform flocculating, putting the L-alanine fermentation liquor subjected to stirring and sedimentation treatment into vacuum rotary-drum filter equipment to perform separating, thereby obtaining a flocculating clarifying solution; crystallizing the flocculating clarifying solution; and drying obtained crystal to obtain the L-alanine. The method provided by the invention adopts the flocculating and sedimentation method to remove impurities such as thallus, proteins, phosphate and sugar residues in the fermentation liquor, so that processes of precipitating calcium salts, removing impurities through a film and the like in the prior art are omitted, and therefore, the extracting process is greatly simplified, equipment investment is reduced and product quality is improved. Crystallization mother liquor and crystal-washing residual water are recycled into a cycle of flocculating, discoloring, concentrating and drying. The extracting method avoids material loss, reduces environment protection pressure, increases the yield, and is energy-saving and environmentally friendly.

Description

technical field [0001] The invention relates to the technical field of fermentation, in particular to a method for extracting L-alanine. Background technique [0002] L-alanine (L-alanine), also known as L-alpha-alanine, is an important natural amino acid, which is the most abundant amino acid in the blood. It is closely related to glucose metabolism and is the main amino acid in the transamination reaction. The amino body has important physiological functions. It is a nutritional supplement, the main component of various compound amino acid infusions, it can also be used as a pharmaceutical intermediate, and it is the main raw material of vitamin B6. It can also be used as a food additive to improve the nutritional value of food and improve the taste of artificial sweeteners. [0003] With the continuous research and development of L-alanine, its application in the fields of food and medicine continues to expand, and there is a growing trend. However, the development of ...

Claims

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Application Information

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IPC IPC(8): C07C227/40C07C229/08
CPCC07C227/40C07C229/08
Inventor 宋山唐思青刘洋殷玉霞程晓伟刘政强赵士雄
Owner QINHUANGDAO HUAHENG BIOENG CO LTD
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