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Marine organism polysaccharide nickel composites, and preparation method and application thereof

A technology of marine biological polysaccharides and compounds, which is applied in the field of marine chemical engineering, can solve problems such as difficult promotion, high cost, and poor drug efficacy, and achieve the effects of expanding application fields, good solubility, and improving antibacterial activity

Inactive Publication Date: 2017-05-31
INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, direct use of chitosan as a single active component has problems such as poor drug efficacy, large dosage, and high cost, so it is difficult to promote

Method used

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  • Marine organism polysaccharide nickel composites, and preparation method and application thereof
  • Marine organism polysaccharide nickel composites, and preparation method and application thereof
  • Marine organism polysaccharide nickel composites, and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Embodiment 1: the preparation of derivative 1

[0032] Add 6.1g of nicotinamide and 5g of acetylacetone into 70mL of absolute ethanol, reflux for 12 hours, spin evaporate the reaction product, wash with cold ethanol, and dry at 60°C to obtain the monoquaternary ammonium ligand.

[0033] Add 0.5g of chitosan with a molecular weight of 1.3 million to 25mL of water, then add 0.5mL of acetic acid dropwise under stirring, and then add 20mL of absolute ethanol; add 2g of monoquaternary ammonium ligand to 50mL of absolute ethanol Add dropwise to the above reaction system of full-chitosan solution, and reflux for 12 hours; then add 2.5g of chloroacetic acid to 10mL of absolute ethanol, dropwise into the above reaction system, react at 60°C for 6h, cool To room temperature, add absolute ethanol for precipitation, precipitate with suction filtration, wash the filter cake with absolute ethanol, and dry at 60°C to obtain a light yellow powder, which is derived from O-carboxymethyl ...

Embodiment 2

[0036] Embodiment 2: the preparation of derivative 2

[0037] Add 6.1g of isonicotinamide and 5g of acetylacetone into 70mL of absolute ethanol, reflux for 12h, spin evaporate the reaction product, wash with cold ethanol, and dry at 60°C to obtain the monoquaternary ammonium ligand.

[0038]Add 0.5g of chitosan with a molecular weight of 1.3 million to 25mL of water, then add 0.5mL of acetic acid dropwise under stirring, and then add 20mL of absolute ethanol; add 2g of monoquaternary ammonium ligand to 50mL of absolute ethanol Add dropwise to the above reaction system of full-chitosan solution, and reflux for 12 hours; then add 2.5g of chloroacetic acid to 10mL of absolute ethanol, dropwise into the above reaction system, react at 60°C for 6h, cool To room temperature, add absolute ethanol for precipitation, precipitate with suction filtration, wash the filter cake with absolute ethanol, and dry at 60°C to obtain a light yellow powder, which is derived from O-carboxymethyl chi...

Embodiment 3

[0041] Embodiment 3: Bacteriostatic activity test of O-carboxymethyl chitosan acetylacetonate nicotinamide class Schiff base nickel complex derivatives of the present invention

[0042] The growth rate method was used to determine the antibacterial activity of the samples against Botrytis cinerea pathogen. Test the inhibitory effect on Botrytis cinerea at three sample concentrations: 100 μg / mL, 200 μg / mL, and 400 μg / mL.

[0043] In the experiment, the same concentration of hop oligosaccharides (commercially available as 2% water) was used as the positive control, and distilled water was used as the negative control. Evenly pour the culture medium containing the sample into petri dishes, each petri dish has a diameter of 9 cm, and after it is completely solidified, inoculate 3 pieces of bacteria cakes with a diameter of 5 mm in each petri dish. After culturing at 27°C for 72 hours, the diameter of the colony was measured, and the bacteriostatic rate of the sample was calculate...

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Abstract

The invention relates to marine organism polysaccharide nickel composites, and a preparation method and application thereof. The marine organism polysaccharide nickel composites are O-carboxymethyl chitosan acetylacetonate nicotinamide Schiff base nickel composite derivatives of which the general formula is disclosed as Formula I, wherein X is pyridyl, and n=4-4000. The polysaccharide nickel composite derivatives disclosed as Formula I can be applied to preparing agricultural bactericides. The O-carboxymethyl chitosan acetylacetonate nicotinamide Schiff base nickel composite derivatives have favorable solubility, and can be dissolved in multiple solvents. On the premise of enhancing the antibacterial effect, the O-carboxymethyl chitosan acetylacetonate nicotinamide Schiff base nickel composite derivatives avoid heavy metal ion residues in soil, thereby having wider application range and having potential application value in the field of agricultural chemicals.

Description

technical field [0001] The invention relates to the technical field of marine chemical engineering, in particular to a marine biological polysaccharide-nickel compound and a preparation method and application thereof. Background technique [0002] Metal preparations have been widely used for a long time because of their advantages such as low price, simple use method, and not easy to produce drug resistance. It is mainly divided into two types: inorganic and organic. Relying on the release of metal ions to interact with -SH, -NH2, -COOH, -OH and other groups in the protein in the pathogenic bacteria, resulting in the death of the pathogenic bacteria. However, improper use of traditional metal preparations can easily cause harm such as phytotoxicity. Therefore, the content and release capacity of metal ions are the main factors that determine the bactericidal and disease-preventing effects of metal preparations, and are also important factors that determine their safety to ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/08A01P3/00
CPCC08B37/003
Inventor 李鹏程刘卫翔秦玉坤刘松邢荣娥于华华李克成高堃
Owner INST OF OCEANOLOGY - CHINESE ACAD OF SCI