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A strain of Pantoea agglomerans and its application

A technology of Pantoea agglomerans and bacterial agents, applied in the field of microorganisms, can solve the problems of little effect and achieve good effect and low cost

Inactive Publication Date: 2019-08-27
YUNNAN AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Correspondingly, the research and development of microbial pesticides for Eupatorium adenophorum has also become a main direction in the field of Eupatorium adenophorum control in recent years, but because Eupatorium adenophorum itself contains a variety of antibacterial substances, the research and development work has achieved little effect so far

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  • A strain of Pantoea agglomerans and its application

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example 1

[0020] Step 1: The ZLSY16 strain that has been screened and purified is preferred, sterilized with standard solid LB medium, added 1% volume of Eupatoria adenophorum extract before solidification, and adjusted the pH value to 7.0, poured plate, After cooling, inoculate ZLSY16 on the culture medium, culture at 28°C for 24 hours, and divide the solid culture medium with bacteria into 1cm 2 A small piece of spare. Step 2: Configure standard liquid LB medium, add 1% Eupatorium adenophorum extract according to the volume ratio after sterilization, adjust the pH value to 7.0, cool down to 35°C, and prepare 1cm of bacteria-carrying medium in the previous step 2 The small pieces of culture medium were inoculated (added) into the liquid medium in proportion, cultured at 28°C for 24 hours, then taken out and cooled to 8°C for 6 hours. Step 3: Take out the refrigerated bacteria-carrying liquid medium, dilute it according to the volume ratio medium: water ratio of 1:10, and spray it on t...

example 2

[0022] Step 1: The screened and purified ZLSY16 strain is preferred, sterilized with standard solid LB medium, add 1% Eupatoria adenophorum extract before solidification, and adjust the pH value to 7.0. Pour the plate and wait for condensation Then ZLSY16 was inoculated onto the medium, and after culturing on the medium at 32°C for 24 hours, the culture medium with bacteria was divided into 1cm 2 A small piece of spare. Step 2: Configure standard liquid LB medium, add 1% Eupatorium adenophorum extract according to the volume ratio after sterilization, adjust the pH value to 7.0, cool down to 35°C, and prepare 1cm of bacteria-carrying medium in the previous step 2 Small pieces of culture medium were inoculated (added) into the liquid culture medium in proportion, cultured at 32°C for 24 hours, then taken out and cooled to 8°C for 6 hours. Step 3: Take out the refrigerated bacteria-carrying liquid medium, dilute it according to the volume ratio medium: water ratio of 1:10, and ...

example 3

[0024] Step 1: The screened and purified ZLSY16 strain is preferred, sterilized with standard solid LB medium, add 1% Eupatoria adenophorum extract before solidification, and adjust the pH value to 7.0. Pour the plate and wait for condensation Then ZLSY16 was inoculated onto the culture medium, and after culturing on the culture medium at 28°C for 24 hours, the bacteria-carrying medium was divided into 1cm 2 A small piece of spare. Step 2: Configure standard liquid LB medium, add 1% Eupatorium adenophorum extract according to the volume ratio after sterilization, adjust the pH value to 7.0, cool down to 35°C, and prepare 1cm of bacteria-carrying medium in the previous step 2 The small pieces of culture medium were inoculated (added) into the liquid medium in proportion, cultured at 28°C for 24 hours, then taken out and cooled to 8°C for 6 hours. Step 3: Take out the refrigerated bacteria-carrying liquid medium, dilute it according to the volume ratio medium: water ratio of 1:...

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Abstract

A strain of Pantoea agglomerans and its application belong to the technical field of microorganisms. The strain was deposited on December 15, 2016 in the General Microbiology Center of China Committee for Microbial Culture Collection (CGMCC for short), address: Institute of Microbiology, Chinese Academy of Sciences, No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing, preservation number: CGMCC No.13452, classification name: Pantoea agglomerans. The bacterial strain of the present invention is screened out from the digestive tract of the parasitic insect Procecidochares utilis Stone of Eupatorium adenophorum, and is used as an active ingredient to prepare a bacterial agent for preventing and controlling Eupatorium adenophorum. The pantoea adenophorum can biologically infect the Eupatorium adenophorum, cause disease of the leaves and stems of the Eupatorium adenophorum and cause rapid death, and the effect is good and the cost is low.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, in particular to a strain of Pantoea agglomerans and its application. Background technique [0002] Eupatorium adenophorum (Eupatorium adenophorum), Asteraceae Eupatorium, perennial herb or subshrub, can quickly form a single superior community, and can inhibit the growth of other plants in various ways, is a worldwide malignant weed, and It is the most harmful exotic plant in my country. Because Eupatorium adenophorum contains a variety of toxic components and odorous components, only a very small number of species such as Eupatorium adenophorum feed on it. In recent years, although people have adopted measures such as putting in obligate natural enemies, herbicides, and plant control to the control of Eupatorium adenophorum, all of them have little effect. [0003] As an efficient, safe, and residue-free modern pesticide, microorganisms play a pivotal role in modern agricultural product...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01N63/02A01P13/00C12R1/01
CPCA01N63/10C12N1/205C12R2001/01
Inventor 吴国星高熹兰明先张某郝凯强唐萍苏发武查友贵李成云
Owner YUNNAN AGRICULTURAL UNIVERSITY