Preparation method of Nereis succinea anti-lung-cancer polypeptides

Abstract

The invention discloses a preparation method of Nereis succinea anti-lung-cancer polypeptides. The method comprises the following steps: (1) sample pretreatment; (2) enzymolysis; (3) ultrafiltration; (4) anion exchange chromatography; (5) SephadexG25 gel chromatography; and (6) high performance liquid chromatography separation purification, thereby finally obtaining the Nereis succinea anti-lung-cancer polypeptides. The method has the advantages of high safety, mild reaction conditions and controllable process. The Nereis succinea anti-lung-cancer polypeptides have high purity, have obvious proliferation inhibiting actions on lung cancer cells A549 and H1299, and have favorable medical application value.

Description

technical field [0001] The invention relates to a marine active polypeptide, in particular to a preparation method of the P. didentate worm anti-lung cancer polypeptide. Background technique [0002] Lung cancer is the malignant tumor with the highest incidence rate in the world. In recent years, the incidence rate and mortality rate have increased year by year, and it has become the leading cause of cancer-related death. About 80% of lung cancer is non-small cell lung cancer, and the proportion of advanced lung cancer reaches 40% to 50% of the total. At present, the treatment of non-small cell lung cancer is surgical resection in the early stage, and chemotherapy in the late stage. However, the toxic side effects and drug resistance of chemotherapy affect the effectiveness of treatment, so finding precise treatment is a hot topic. The ocean is a huge treasure trove of drugs. It has become a hot spot to search for active substances from the ocean, and to separate and obtain...

AI Technical Summary

Problems solved by technology

However, there are few studies on the enzymatic hydrolysis of peptides by nereis worm, and the inhibitory activity on non-small cell lung cancer A549 cells has not been reported yet.

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