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Method for building genetic map of bamboo plants
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A genetic map and technology of bamboo plants, which are applied in the field of rapid construction of bamboo genetic map based on segregated populations of self-inbred generation, and rapid construction of high-density genetic linkage map of bamboo plants, can solve the problem of restricting bamboo plants and difficult to obtain bamboo plants. problems such as genetic maps, to achieve the effect of reducing the cost and time of genotyping, avoiding the contamination of progeny materials, and simplifying the construction process
Inactive Publication Date: 2017-05-31
KUNMING INST OF BOTANY - CHINESE ACAD OF SCI
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Therefore, it is also difficult to obtain a complete bamboo genetic map using traditional low-generation (F1 generation) hybrid populations, which seriously limits the development of genetic breeding of bamboo plants, such as linkage analysis between economic traits and markers, gene mapping and molecular marker-assisted selection breeding
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Embodiment 1
[0054] The construction of the self-bred genetic population of Mazhu:
[0055] The present embodiment selects the Ma bamboo (Dendrocalamus latiflorus Munro) that is widely distributed in the provinces of southern China as the experimental material, and the construction of the Ma bamboo genetic mapping population includes the following steps:
[0056] The 1st step, the collection of Ma bamboo parent plant:
[0057] Ma bamboo is a kind of clump bamboo. Several bamboo poles growing in a clump are propagated from one mother plant. We found a clump of Bamboo japonica in the early flowering stage in Mile City, Yunnan Province, and transplanted it to the greenhouse area of Kunming Institute of Botany, Chinese Academy of Sciences as a standby parent.
[0058] 2nd step, the selfing of bamboo parent plant:
[0059] First, use scissors to cut off the pollinated seeds and the exposed pistils (pollination status is unknown), and only keep the stamens and unreleased pistils; then, colle...
[0065] Randomly select 188 progeny individuals from the S1 population obtained in Example 1 for the construction of the genetic map of bamboo. Using the modified CTAB method to extract the whole genomeDNA of the young leaves of the parents and offspring of Mazhu:
[0066] (1) Take 200ml 2% CTAB and preheat at 60°C, add 2‰ dithiothreitol and mix well,
[0067] (2) Weigh 80 mg of fresh young leaves, add liquid nitrogen and quickly grind them into powder, transfer to a 2ml small centrifuge tube filled with 1ml 60°C CTAB buffer solution, oscillate and mix well, 60°C water bath, 50min, during the mixing Second-rate,
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Abstract
The invention provides a method for building a genetic map of bamboo plants. The method comprises the following steps: collection of bamboo free-flowering stock plants, building and cultivation of a self-cross (S1 generation) segregation population, extraction of parent and daughterDNA, simplification of genomic sequencing and genotyping, as well as application of an F2-generation populationsimulation strategy and genetic map building software for Fe-generation population model analysis to acquire the genetic map of the bamboo plants. The method has the advantages that the S1-generation segregation population and the F2-generation population simulation strategy are combined for building of the genetic map, so that the shortcoming of difficult mutual cross of bamboos is overcome, and accuracy and reliability of the parent source of the population material is guaranteed. A new concept and method are provided for building the bamboo genetic map by applying the free-flowering material, so that the building progress of the bamboo genetic map is quickened. The method has favorable actual application value and application prospect in the field of bamboo plantmolecular breeding.
Description
technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a rapid construction method of a high-density genetic linkage map of bamboo plants, which is a rapid construction method of a bamboo genetic map based on a self-bred generation segregation population. Background technique [0002] Bamboo plants belong to the Poaceae Bambooideae (Bambusoideae), and most of them are lignified grasses. There are about 88 genera and more than 1400 species in the world, mainly distributed in tropical and subtropical regions of Asia, Africa and America (Li et al., 2006). China is the most diverse region of woody bamboo in the world, with about 37 genera and more than 500 species. There are currently 22 million hectares of bamboo forests in the world 2 , accounting for about 1% of the world's total forest area. Among them, China is the country with the largest bamboo forest area, with an area of about 3.79 million hm 2 , Known as the "King...
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IPC IPC(8): C12Q1/68
CPCC12Q1/6869C12Q2525/191C12Q2531/113
Inventor 杨国骞郭岑勾雪娇王玉娇李德铢郭振华
Owner KUNMING INST OF BOTANY - CHINESE ACAD OF SCI
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