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Detection method of CEA (carcinoembryonic antigen) concentration

A detection method, carcinoembryonic antigen technology, applied in the field of biosensing, can solve the problems of time-consuming and labor-intensive, high technical requirements, and achieve the effect of improving detection sensitivity, wide detection range, and promoting signal amplification

Inactive Publication Date: 2017-05-31
CENT SOUTH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Although these detection methods are sensitive and effective to a certain extent, they have high technical requirements, are time-consuming and labor-intensive, and have certain requirements for instruments, each of which has certain limitations.

Method used

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  • Detection method of CEA (carcinoembryonic antigen) concentration
  • Detection method of CEA (carcinoembryonic antigen) concentration
  • Detection method of CEA (carcinoembryonic antigen) concentration

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Embodiment 1

[0042] A kind of embodiment of the detection method of CEA of the present invention, its principle schematic diagram is as figure 1 shown. The detection method specifically includes the following steps:

[0043] (1) Synthesis of gold nanorod carrier: first pass excess NaBH 4 Reduce the concentration of 1-2% HAuCl at 30°C 4 The solution obtains a gold nanometer seed solution. Then, add 1.1mL HAuCl to 50mL 0.1M cetyltrimethylammonium bromide solution 4 (1% w / w), 0.4 mL AgNO 3 (10mM) and 0.29mL ascorbic acid (0.1M). Finally, 0.06 mL of synthesized nano-gold seed solution was added to the above solution. The mixed solution continued to react for 24 hours to obtain the gold nanorod carrier.

[0044] (2) Modifying the CEA aptamer on the gold nanorod carrier: the synthesized gold nanorod carrier was dispersed in 10 M CEA aptamer solution and reacted at room temperature for 2 h, and the unreacted CEA aptamer was removed by centrifugal washing to obtain the CEA aptamer. Ligand...

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Abstract

The invention discloses a detection method of the CEA (carcinoembryonic antigen) concentration. According to the method, CEA aptamer modified gold nanometer rods are used as electrochemical probes; firstly, a CEA antibody-CEA-aptamer / gold nanometer rod three-layer sandwich type structure is firstly built; then, on the basis of a principle that phosphate radicals on the aptamers can take a reaction with sodium molybdate to generate oxidation-reduction current; the electrochemical method detection of the CEA concentration is realized. The method has the advantages of high selectivity, high sensitivity, low detection limit, wide detection range and the like.

Description

technical field [0001] The invention relates to a method for detecting the concentration of carcinoembryonic antigen (CEA), in particular to a construction of a CEA antibody-CEA-CEA aptamer / gold nanorod three-layer sandwich structure and a method for detecting the concentration of CEA; It belongs to the field of biosensing technology. Background technique [0002] Carcinoembryonic antigen is a broad-spectrum tumor marker, which can reflect the existence of a variety of tumors to people. It is a good tumor marker for the judgment of curative effect, disease development, monitoring and prognosis estimation of colorectal cancer, breast cancer and lung cancer thing. 97% of healthy adults have serum CEA concentrations below 2.5g / L, and 45-80% of patients with primary colon cancer have elevated CEA levels. In addition to primary colon cancer, the positive rate of pancreatic cancer, bile duct cancer, gastric cancer, esophageal cancer, adenocarcinoma, lung cancer, breast cancer an...

Claims

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Application Information

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IPC IPC(8): G01N33/574G01N27/48G01N27/327
CPCG01N27/3277G01N27/48G01N33/57473
Inventor 阳明辉李亭司忠洲
Owner CENT SOUTH UNIV
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