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High-efficiency banana tissue culture strong seedling culture method

A cultivation method, banana technology, applied in horticultural methods, botanical equipment and methods, agricultural machinery and equipment, etc., can solve problems such as poor results

Inactive Publication Date: 2017-06-20
黄庆辉
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  • Summary
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  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, banana is a kind of popular fruit, and it is also the main food in some areas. It has a huge market demand. It is necessary to carry out research on rapid propagation of banana, which can not only solve the huge market demand, but also be To a certain extent, good banana varieties are preserved. Tissue culture is one of the methods for rapid propagation of bananas. However, the existing banana tissue culture has poor seedling effect and cannot meet the current growth rate of demand.

Method used

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Experimental program
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Embodiment Construction

[0013] The present invention will be further described below in conjunction with specific embodiments.

[0014] Step S10, prepare the culture medium according to the following formula: use the B5 medium as the base medium, add the concentration of coumarin to 50 mg / L, the concentration of NAA (naphthalene acetic acid) to 1 mg / L, and the concentration of lanthanum nitrate to 3 g / L L, the concentration of agar powder is 10g / L, the concentration of white granulated sugar is 30g / L;

[0015] Step S20, select normal and healthy banana tissue culture seedlings, inoculate the banana tissue culture seedlings into the tissue culture bottle equipped with the medium prepared in step S10 in the ultra-clean workbench, and transfer to the tissue culture room;

[0016] Step S30, adjusting the ambient temperature in the tissue culture room to 28°C to 30°C, using natural light of 2000lux light intensity, blue light enhanced light source and red light enhanced light source to irradiate the banan...

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Abstract

The invention provides a high-efficiency banana tissue culture strong seedling culture method. The method is as below: selecting a B5 culture medium as a basal culture medium; adjusting a hormone formula in the culture medium; adding rare earth elements and coumarins; and adding enhanced color light sources in a strong seedling culture process, thereby greatly improving the strong seedling effects of banana tissue culture seedlings. The method has the advantages of good strong seedling effects and high chlorophyll content in leaves.

Description

technical field [0001] The invention belongs to the technical field of banana tissue culture, and in particular relates to an efficient method for cultivating strong banana seedlings through tissue culture. Background technique [0002] Banana (scientific name: Musa nana Lour.) Musa family Musa plant, also refers to its fruit. It is widely cultivated and eaten in tropical areas. Savory and nutritious, bananas are harvested year-round and are also valued in temperate regions. The plant is a large herb, emanating from the rhizome, forming a false stem 3-6 meters (10-20 feet) high from the lower part of the leaf sheath; the leaves are oblong to oval, and some are 3-3.5 meters long (10 ~11.5 feet), 65 cm (26 inches) wide, with 10-20 clustered stem tops. drooping spikes [1] , pulled out from the top of the false stem, with many flowers, light yellow; infructescence drooping, resulting in 10-20 clusters, about 50-150. After the plant bears fruit, it dies, and the suckers grow...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00A01G7/04
CPCA01G7/04A01H4/00A01H4/001Y02P60/14
Inventor 不公告发明人
Owner 黄庆辉
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