Application of perhexiline/oxaliplatin drug combination in aspect of treatment of stomach cancer and colorectal cancer

A technology of oxaliplatin and perhexiline, applied in the field of medicine, can solve the problems such as no research and report on the combination of perhexiline and oxaliplatin, and achieve inhibition of growth and proliferation, and significant therapeutic effect. , the effect of inducing apoptosis

Active Publication Date: 2017-06-20
SUN YAT SEN UNIV CANCER CENT
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are no reports on the application of perhexiline in other diseases such as gastric cancer and intestinal cancer
[0005] In addition, oxa

Method used

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  • Application of perhexiline/oxaliplatin drug combination in aspect of treatment of stomach cancer and colorectal cancer
  • Application of perhexiline/oxaliplatin drug combination in aspect of treatment of stomach cancer and colorectal cancer
  • Application of perhexiline/oxaliplatin drug combination in aspect of treatment of stomach cancer and colorectal cancer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Example 1 Effect of Perhexiline on Gastric Cancer and Colorectal Cancer Cells and Their Tumor Formation

[0033] 1. Experimental materials

[0034] (1) Drug: perhexiline maleate is commonly used in clinical practice, and this example uses perhexiline maleate as the experimental drug.

[0035] The chemical structural formula of perhexiline maleate is as follows:

[0036]

[0037] (2) Cancer cells:

[0038] Gastric cancer cells: human gastric cancer cells HGC27 and MGC803;

[0039] Colorectal cancer cells: human colon cancer cells HCT 116 and human colon adenocarcinoma cells DLD-1.

[0040] (3) Commercially available apoptosis kits.

[0041] 2. Experimental grouping

[0042] (1) Control group: blank control, that is, the cancer cells were not treated with any drugs.

[0043] (2) Experimental group: cancer cells were treated with perhexiline maleate.

[0044] 3. The effect of perhexiline maleate on the apoptosis of gastric cancer and colorectal cancer cells detec...

Embodiment 2

[0062] Example 2 Effect of perhexiline on the proliferation of gastric cancer and colorectal cancer cells

[0063] 1. Experimental materials

[0064](1) Drugs: Same as Example 1.

[0065] (2) Cancer cells: same as in Example 1.

[0066] (3) Commercially available MTS kits.

[0067] 2. Detect the activity of HGC27 and MGC803 cells, HCT 116 and DLD-1 cells after perhexiline maleate treatment by MTT method

[0068] (1) Pave gastric cancer and colorectal cancer cells in a 96-well plate. After they adhere to the wall, add medium containing 0 μM, 5 μM, 10 μM, and 20 μM perhexiline maleate to treat the cells, and then treat the cells at 24h and 48h , 72h by MTT method to detect HGC27 and MGC803 cells, HCT 116 and DLD-1 cell activity.

[0069] The specific method is as follows:

[0070] 1) Digest HGC27 and MGC803 cells, HCT 116 and DLD-1 cells in the logarithmic growth phase with trypsin, and take 4×10 3 Cells were spread evenly into 96-well plates.

[0071] 2) After the cells ...

Embodiment 3

[0075] Example 3 Effect of Perhexiline on Metastasis of Gastric Cancer and Colorectal Cancer Cells

[0076] 1. Experimental materials

[0077] (1) Drugs: Same as Example 1.

[0078] (2) Gastric cancer cells: same as in Example 1.

[0079] (3) Transwell chambers with and without pre-coated Matrigel.

[0080] 2. The effect of perhexiline maleate treatment on the invasion and migration of HGC27 and MGC803 cells, HCT116 and DLD-1 cells was detected by Transwell chamber

[0081] (1) Spread gastric cancer and colorectal cancer cells in a six-well plate, and after they adhere to the wall, add 0 μM, 3 μM and 4 μM (or 5 μM) perhexiline maleate to treat gastric cancer cells for 48 hours, add 0 μM, 4 μM and Intestinal cancer cells were treated with 6 μM perhexiline maleate for 48 hours, and the effects of perhexiline maleate treatment on the invasion and migration of HGC27 and MGC803 cells, HCT 116 and DLD-1 cells were detected by Transwell chamber.

[0082] The specific method is as...

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PUM

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Abstract

The invention discloses application of perhexiline/oxaliplatin drug combination in the aspect of treatment of stomach cancer and colorectal cancer. The research shows that the perhexiline is capable of obviously inhibiting the growth, proliferation and clone formation capacities of the stomach cancer and colorectal cancer cells, inducing the apoptosis of the stomach cancer and colorectal cancer cells and inhibiting the invasion and transfer capacities of the stomach cancer and colorectal cancer cells, has obvious treatment effects on stomach cancer and colorectal cancer, and therefore, can be used in drugs for treating stomach cancer and intestinal cancer. The invention provides new application of perhexiline, thereby widening the clinical application fields of perhexiline; and the invention also provides a new scheme of perhexiline/oxaliplatin drug combination in treating stomach cancer and colorectal cancer, thereby increasing the options for chemotherapeutic schemes of stomach cancer and colorectal cancer, and having favorable clinical application prospects.

Description

technical field [0001] The invention belongs to the technical field of medicine. More specifically, it relates to the application of perhexiline and oxaliplatin in the treatment of gastric cancer and colorectal cancer. Background technique [0002] Gastric cancer and intestinal cancer are common gastrointestinal tumors in the world, ranking first among all kinds of malignant tumors in my country, and one of the main causes of death worldwide, and there are great difficulties in clinical treatment. [0003] Studies have shown that appropriate combination drugs can play a synergistic effect and achieve better therapeutic purposes. However, there are many kinds of drugs, and the interactions between different drugs are also different, and poor coordination may even affect the efficacy of drugs or increase toxicity. Therefore, the screening research of combined drugs in cancer treatment is of great significance. [0004] Perhexiline is a calcium ion channel antagonist, which ...

Claims

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Application Information

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IPC IPC(8): A61K31/555A61K31/4458A61P35/00A61P1/00
CPCA61K31/4458A61K31/555A61K2300/00
Inventor 徐瑞华鞠怀强王运
Owner SUN YAT SEN UNIV CANCER CENT
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