Supercharge Your Innovation With Domain-Expert AI Agents!

Preparation method of conchiolin, conchiolin prepared by method and application of conchiolin

A pearl protein and pearl technology, which is applied in the fields of medicine and cosmetics, can solve the problems of lack of systematic research, low utilization efficiency of pearl active ingredients, unclear basis of medicinal effect substances, etc., and achieves the effects of simple process, easy industrial production and low cost.

Inactive Publication Date: 2017-06-20
SHANGHAI INST OF MATERIA MEDICA CHINESE ACAD OF SCI
View PDF5 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the medicinal material basis of pearl whitening activity is still unclear, lack of systematic research, and the development of related products is basically blank, resulting in blindness in the use of pearls and low utilization efficiency of active ingredients

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Preparation method of conchiolin, conchiolin prepared by method and application of conchiolin
  • Preparation method of conchiolin, conchiolin prepared by method and application of conchiolin
  • Preparation method of conchiolin, conchiolin prepared by method and application of conchiolin

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] Embodiment 1: the extraction of pearl protein

[0048] 1. Extraction of water-soluble protein:

[0049] The extraction of water-soluble protein adopts the cold soaking method. The specific method is: take 100g of medicinal material powder (according to the requirements of the "Chinese Pharmacopoeia" 2010 edition, pearls are crushed into the finest powder), add 10 times the volume of water, and cold soak at 4°C for 24 hours. The extract is filtered, and the filtrate is treated by ammonium sulfate precipitation or direct freeze-drying, and the above-mentioned product is taken, and the amino acid content determination method reported in the literature [Journal of Separation Science.2015,38:1552-1560 .] to measure the content of amino acids in the protein obtained by the two methods. The results are shown in Table 1. It can be seen that the content of amino acids in the protein obtained by the direct freeze-drying method is higher than that of ammonium sulfate precipitation...

Embodiment 2

[0066] Embodiment 2: the preparation of keratin in pearl

[0067] The preparation of keratin, the concrete method is: get medicinal material powder (according to " Chinese Pharmacopoeia " 2010 edition requirements, pearl is pulverized into the finest powder) 100g, add 10% acid (hydrochloric acid or acetic acid) of 10 times volume, under stirring Treat at 4°C for 12 hours, centrifuge at 12000 rev / min at a high speed, and freeze-dry the obtained precipitate to obtain keratin.

[0068] Investigated 10% hydrochloric acid and 10% acetic acid, took the keratin shell protein prepared above, and measured the obtained protein respectively according to the content determination method of amino acid reported in the literature [Journal of Separation Science.2015,38:1552-1560.] The content of amino acids in the protein, the results are shown in Table 5, as can be seen from the results, when using hydrochloric acid, the content of amino acids in the protein is higher than that of acetic aci...

Embodiment 3

[0075] Example 3: Determination of the whitening activity of the pearl protein prepared in Example 1

[0076] Preparation of phosphate buffer solution:

[0077] Take 13.40g of disodium hydrogen phosphate in a 250mL measuring bottle, add deionized water to dissolve, make up to the mark, and shake well; take 6.90g of sodium dihydrogen phosphate in a 250mL measuring bottle, add deionized water to dissolve, make up to the mark , shake well; accurately pipette 50mL of each of the above two solutions into a 250mL measuring bottle, add deionized water to dilute to the mark, and obtain a phosphate buffer solution with a pH of 6.8.

[0078] Preparation of L-tyrosine solution:

[0079] Take 3.7mg of L-tyrosine and weigh it accurately, add phosphate buffer solution to dissolve in a 50mL measuring bottle, adjust the volume to the mark, and shake well to obtain an L-tyrosine solution with a concentration of 74μg / mL.

[0080] Preparation of tyrosinase solution:

[0081] The specification...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
particle sizeaaaaaaaaaa
Login to View More

Abstract

The invention discloses a preparation method of conchiolin, the conchiolin prepared by the method and application of the conchiolin. The invention aims at determining the pharmacodynamic material basis of the whitening activity of a pearl; discovered through research, one kind of protein in the pearl has better anti-tyrosinase activity, and can be used as a raw material for a whitening product to be used for a medicine and a cosmetic; the preparation method of the kind of the conchiolin is investigated; a preparation process is determined.

Description

technical field [0001] The invention relates to the technical fields of medicine and cosmetics, in particular, the invention relates to a preparation method of pearl protein, pearl protein prepared by the method and application thereof. Background technique [0002] Pearls are recorded in the Chinese Pharmacopoeia (2015 edition), and are bivalves such as Pteria martensii (Dunker), Hyriopsis cumingii (Lea), or Cristaria plicata (Leach), which are pearl oysters. Formed by stimulation, its nature and flavor are sweet, salty, and cold; it enters the heart and liver meridian. Pearl is a traditional Chinese medicine in my country. Modern pharmacological studies have shown that pearls have the effects of calming the nerves, anti-oxidation, anti-inflammation, promoting wound healing, and lowering blood pressure. Pearl is a shellfish marine medicine, which contains more than 90% calcium carbonate. In addition, it also contains a small amount of water-soluble protein, keratin, peptide...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/435C07K1/30A61K38/17A61K8/65A61P17/00A61Q19/02A23L33/18
CPCA23V2002/00A61K8/65A61K38/00A61K2800/782A61Q19/02C07K14/43504A23V2200/318
Inventor 吴婉莹张静娴李尚蓉
Owner SHANGHAI INST OF MATERIA MEDICA CHINESE ACAD OF SCI
Features
  • R&D
  • Intellectual Property
  • Life Sciences
  • Materials
  • Tech Scout
Why Patsnap Eureka
  • Unparalleled Data Quality
  • Higher Quality Content
  • 60% Fewer Hallucinations
Social media
Patsnap Eureka Blog
Learn More

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2025 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com