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Idiopathic membranous nephropathy duplex detection kit

A detection kit and technology for membranous nephropathy, applied in measuring devices, disease diagnosis, instruments, etc., can solve the problems of high detection cost, time-consuming, unfavorable large-scale screening of idiopathic model nephropathy, etc., to achieve simple operation, High sensitivity and fast response effect

Inactive Publication Date: 2017-06-23
上海埃文生物科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0009] Chinese invention patent CN201610201970.7 discloses an ELISA detection kit for idiopathic membranous nephropathy and its detection method. The patent uses the direct ELISA method to quantitatively detect anti-PLA2R and anti-THSD7A autoantibodies in human serum for screening For idiopathic membranous nephropathy, this method takes a long time (2-3 hours); it needs to be detected in professional places, professionals and equipment; the detection cost is high, which is not conducive to the large-scale screening of idiopathic model nephropathy at the grassroots level

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Embodiment Construction

[0029] Hereinafter, the present invention will be further described in conjunction with the embodiments and drawings, which are only for explanation and not intended to limit the protection scope of the present invention.

[0030] The process of preparing the detection card for the joint detection and screening of idiopathic membranous nephropathy by anti-PLA2R Ab and anti-THSD7A Ab is as follows:

[0031] 1 main material

[0032] 1.1 Preparation of PLA2R protein and THSD7A protein

[0033] 1.1.1 Primer design

[0034] According to NCBI and related literature reports, primers were designed for PLA2R (Accession: Q13018) and THSD7A (Accession NP_056019.1) genes respectively. The specific sequences of the primers are as follows:

[0035] PLA2R Gene

[0036] F1 is shown in SEQ ID NO.1,

[0037] R1 is shown in SEQ ID NO.2.

[0038] THSD7A Gene

[0039] F1 is shown in SEQ ID NO.3,

[0040] R1 is shown in SEQ ID NO.4.

[0041] 1.1.2 Expression vector construction

[0042] Th...

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Abstract

The invention relates to an idiopathic membranous nephropathy duplex detection kit which comprises a, an anti-PLA2R Ab test strip and b, an anti-THSD7A Ab test strip, wherein the anti-PLA2R Ab test strip is prepared from an anti-lgG4 gold pad containing a color-marked mouse anti-human IgG4Fc secondary antibody (anti-lgG4 Ab), a detection line containing PLA2R protein and a nitrocellulose membrane containing a goat anti-mouse IgG quality control line, and the anti-THSD7A Ab test strip is prepared from an anti-lgG4 gold pad containing a color-marked mouse anti-human IgG4Fc secondary antibody (anti-lgG4 Ab), a detection line containing THSD7A protein and a nitrocellulose membrane containing a goat anti-mouse IgG quality control line. The duplex detection kit disclosed by the invention can be used to screen idiopathic membranous nephropathy by detecting anti-PLA2R Ab and anti-THSD7A Ab in whole blood / serum / plasma, and has the advantages of simpleness and convenience in operation, quick reaction, high sensitivity, strong specificity, suitability for field test, economy, practicability and the like.

Description

[0001] [technical field] [0002] The invention relates to the field of biotechnology, in particular to a detection kit for idiopathic membranous nephropathy. [0003] [Background technique] [0004] More than 500 million people around the world suffer from chronic kidney disease to varying degrees. Chronic kidney disease has become another important disease that threatens human health after tumors, cardiovascular and cerebrovascular diseases, and diabetes, and has become an important global public health problem. In my country, the incidence of chronic kidney disease is 10% to 13%. China has a large population, and the total number of patients with chronic kidney disease is huge, which has undoubtedly become a heavy social and economic burden in our country. According to the etiology, membranous nephropathy can be divided into idiopathic and secondary membranous nephropathy. The diagnosis depends on clinical manifestations, histological examination of nephrocentesis or electr...

Claims

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Application Information

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IPC IPC(8): G01N33/558
CPCG01N33/558G01N2800/347
Inventor 杨万春张笑人王伦善沈晓宝陈云
Owner 上海埃文生物科技有限公司