White radish juice
A white radish juice, white radish technology, applied in plant cells, horticulture, botanical equipment and methods, etc., can solve the problems of low juice yield, difficult to soften white radish tissue, complex structure, etc. The effect of natural taste and high nutrient content
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Embodiment 1
[0028] (1) Get the main root of white radish and soak it in the L-ascorbic acid solution of 80mg / L for 5 minutes, then sterilize it with 75% ethanol for 2 minutes, wash it with sterile water, cut it into aseptic thin slices containing cambium, and transfer it to Induction medium MS solid medium + 200mg / LCaCl 2 +1300mg / LNH 4 NO 3 +0.8mg / L glycine+0.3mg / LNAA cultured for 22 days until explants grow;
[0029] (2) Subculture the explants to the growth medium MS liquid medium+2mg / L inositol+0.1mg / LVB 1 +0.5mg / LVB 6 +0.5mg / L niacin+2% sucrose cultured for 26 days to obtain white radish cambium stem cells;
[0030] (3) Transplant white radish cambium stem cells into liquid medium MS liquid medium+2mg / L inositol+0.1mg / LVB 1 +0.5mg / LVB 6 +0.5mg / L niacin+2% sucrose, 80rpm, 12h light / 12h dark photoperiod for subculture for 22 days;
[0031] (4) The cell line obtained from the culture in step (3) is moved into an airlift bioreactor to continue amplified culture, and the culture con...
Embodiment 2
[0034] (1) Get the main root of white radish and soak it in the L-ascorbic acid solution of 90 mg / L for 5 minutes, then disinfect it with 75% ethanol for 2 minutes, wash it with sterile water, cut it into aseptic thin slices containing cambium, and transfer it to Induction medium MS solid medium + 210mg / LCaCl 2 +1350mg / LNH 4 NO 3 +1.2mg / L glycine+0.4mg / LNAA cultured for 23 days until explants grow;
[0035] (2) Subculture the explants to the growth medium MS liquid medium+3mg / L inositol+0.15mg / LVB 1 +0.55mg / LVB 6 + 0.55mg / L niacin + 2.4% sucrose cultured for 27.5 days to obtain white radish cambium stem cells;
[0036] (3) Transplant white radish cambium stem cells into liquid medium MS liquid medium+3mg / L inositol+0.15mg / LVB 1 +0.55mg / LVB 6 +0.55mg / L niacin+2.4% sucrose, 85rpm, 12h light / 12h dark photoperiod for subculture for 23 days;
[0037] (4) The cell line obtained from the culture in step (3) is moved into an airlift bioreactor to continue amplified culture, and...
Embodiment 3
[0040] (1) Take the main root of white radish and soak it in the L-ascorbic acid solution of 100mg / L for 5 minutes, then sterilize it with 75% ethanol for 2 minutes, wash it with sterile water, cut it into aseptic thin slices containing cambium, and transfer it to Induction medium MS solid medium + 220mg / LCaCl 2 +1400mg / LNH 4 NO 3 +1.6mg / L glycine+0.5mg / LNAA cultured for 24 days until explants grow out;
[0041] (2) Subculture the explants to the growth medium MS liquid medium+4mg / L inositol+0.2mg / LVB 1 +0.6mg / LVB 6 +0.6mg / L niacin+2.8% sucrose cultured for 29 days to obtain white radish cambium stem cells;
[0042] (3) Transplant white radish cambium stem cells into liquid medium MS liquid medium+4mg / L inositol+0.2mg / LVB 1 +0.6mg / LVB 6 +0.6mg / L niacin+2.8% sucrose, 90rpm, 12h light / 12h dark photoperiod for subculture for 24 days;
[0043](4) The cell line obtained from the culture in step (3) is moved into an airlift bioreactor to continue amplified culture, and the cu...
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