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Preparation method of oral insulin nanocomposite of acidic polysaccharide penetrating peptide of Apocynum apocynum

A nanocomposite and acidic polysaccharide technology, applied in the field of biomedicine, can solve the problems of reducing insulin bioavailability, slow onset of oral insulin, and unstable absorption efficiency, etc., to improve bioavailability, improve islet cell viability, improve The effect of absorption efficiency in the small intestine

Inactive Publication Date: 2020-08-28
SUN YAT SEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, chitosan, as an aminopolysaccharide, is alkaline in itself, and in the process of preparing oral insulin nanocomposites, it will irreversibly damage the activity of insulin and seriously reduce the bioavailability of insulin.
Looking at the existing oral insulin nanosystems, there are mainly problems that need to be improved, such as low bioavailability, slow onset of oral insulin, and unstable absorption efficiency.

Method used

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  • Preparation method of oral insulin nanocomposite of acidic polysaccharide penetrating peptide of Apocynum apocynum
  • Preparation method of oral insulin nanocomposite of acidic polysaccharide penetrating peptide of Apocynum apocynum

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Embodiment Construction

[0023] (1) Preparation of oral insulin nanocomposite with acidic polysaccharide penetrating peptide of Apocynum apocynum:

[0024] 10 mL Apocynum acidic polysaccharide aqueous solution, 0.1 mg / mL, placed on a magnetic stirrer, added to the rotor, while slowly adding 0.8 mg / mL insulin solution with pH=4 and 0.1 mg / mL penetrating peptide solution, adding speed 1 mL / min, after the dropwise addition is complete, continue to stir for 5 min. Take 1 mL of the solution and centrifuge in a 100k ultrafiltration tube to measure the encapsulation efficiency of insulin. The result shows that the encapsulation efficiency of insulin reaches 98%. Take 1 mL of the solution and measure the nanoparticle size on a Malvern particle sizer, the results are as follows: figure 1 As shown, the particle size is 60 nm.

[0025] (2) Evaluation of the hypoglycemic effect of oral insulin nanocomposites of Apocynum acidic polysaccharide penetrating peptide:

[0026] Adult male SD rats were injected with 5...

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Abstract

The invention discloses a method for preparing a stable and efficient apocynum acidic polysaccharide cell-penetrating peptide oral insulin nanocomposite through interaction of positive and negative charges. The apocynum acidic polysaccharide cell-penetrating peptide oral insulin nanocomposite prepared by the method is good in histocompatibility and simple in preparation method, and has the advantage of higher bioavailability.

Description

technical field [0001] The invention relates to the technical field of biomedicine, and more specifically, relates to a preparation method of oral insulin nanocomposite of acidic polysaccharide penetrating peptide of apocynum apocynum. Background technique [0002] With the development of society and the continuous improvement of people's living standards, "wealth diseases" such as obesity and diabetes are showing a rapid growth trend in society. In order to effectively treat these affluence diseases, researchers have carried out a lot of theoretical research and drug development. At this stage, insulin is still the most widely used and most effective hypoglycemic drug. Due to the protein properties of insulin, oral administration cannot exert the biological activity of lowering blood sugar. Most diabetic patients need frequent injections every day, which brings a lot of inconvenience to patients’ daily life, and there are also certain risks such as allergic reactions and in...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K38/28A61K47/36A61K47/42A61P3/10A61K31/715
CPCA61K31/715A61K38/28A61K47/36A61K47/42A61K2300/00
Inventor 孙成新刘利新陈永明何治宇刘志佳
Owner SUN YAT SEN UNIV