Method for improving RNA virus resistance of plants

A RNA virus and plant technology, applied in the field of improving the ability of plants to resist RNA viruses, can solve problems such as the lack of an anti-RNA virus system, and achieve the effect of improving disease resistance and improving resistance

Pending Publication Date: 2017-07-11
SOUTH CHINA AGRI UNIV
View PDF5 Cites 4 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the anti-virus systems using these gene editing technologies are all aimed at DNA vir

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for improving RNA virus resistance of plants
  • Method for improving RNA virus resistance of plants
  • Method for improving RNA virus resistance of plants

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0052] Example 1

[0053] This embodiment discloses a method for cultivating plants with improved resistance to RNA viruses. The method includes the following steps:

[0054] S1 selects a number of target sequences from the genome sequence of the RNA virus to be resisted, and designs and synthesizes DNA fragments that are reverse complementary to the target sequence for the target sequences. The DNA fragments are double-stranded DNA. The sequence arrangement rule of the sequence is 5'-N X -C-3’;

[0055] Where N X Represents X consecutive ribonucleotides, N represents any of A, G, C, U, 10≤X≤30, and X is an integer;

[0056] S2 respectively construct several of the DNA fragments obtained in step S1 into a vector for expressing CRISPR / Cas9 nuclease to obtain several recombinant vectors;

[0057] In S3, several of the recombinant vectors are respectively introduced into recipient plants, and plants with improved resistance to the RNA virus are obtained from the recipient plants into whic...

Example Embodiment

[0074] Example 2

[0075] Example 2 is based on the method in Example 1. Cucumber mosaic virus (CMV) is selected as the RNA virus to be resisted, and Arabidopsis thaliana is selected as the plant body to be attacked by CMV, indicating that the establishment of improved plant resistance to RNA virus Method of ability.

[0076] The genome of CMV is single-stranded RNA. In this example, multiple specific sgRNAs are designed for the viral RNA genome to guide FnCas9 to reduce the accumulation of CMV in plants.

[0077] 1. Construction of Binary Vector of Dicotyledon CRISPR / Cas9 System

[0078] 1. According to the second-generation sequencing results of cucumber mosaic virus (CMV) (sequence XX-XX), select X target fragments from the CMV genome (Table 1). In addition, a non-viral sequence target fragment was constructed as a control.

[0079] Table 1 Target fragment design

[0080]

[0081]

[0082] Among them, the underlined part in Table 1 is the PAM sequence.

[0083] 2. According to the ta...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The present invention discloses a method for improving the RNA virus resistance of plants. The method comprises: S1, selecting a plurality of target sequences from the genome sequence of RNA virus to be resisted, and respectively designing and synthesizing DNA fragments reversely complementary to the target sequences, wherein the DNA fragments are double-stranded DNA, and the arrangement rule of the target sequences is 5'-NX-C-3'; S2, respectively constructing the DNA fragments obtained in step the S1 into vectors for expressing CRISPR/Cas9 nuclease to obtain a plurality of recombinant vectors; and S3, respectively transforming the recombinant vectors into acceptor plants, and obtaining the plant having the improved RNA virus resistance from the acceptor plants having the transformed recombinant vectors. According to the present invention, the application of the CRISPR/CAS9 system in the effective inhibition of the CMV proliferation in the plant is limited so as to improve the disease resistance of plants.

Description

technical field [0001] The invention relates to the technical field of plant cultivation, in particular to a method for improving the ability of plants to resist RNA viruses. Background technique [0002] Plant virus diseases can cause harm to many plants, greatly affect the growth of plants, and cause serious losses in agricultural production. According to the genome composition of the virus, it is divided into two types: RNA virus and DNA virus. Among them, plant RNA viruses widely exist in nature and have a very wide range of hosts. Most of the known RNA viruses can spread through various channels, causing huge losses to economic crops. [0003] Taking cucumber mosaic virus as an example, it is a very serious plant virus disease transmitted by aphids. The virus particle is an equiaxed icosahedron, and the genome is a three-molecule linear positive-sense RNA. Cucumber mosaic virus is one of the plant viruses with the largest host range, the widest distribution and the m...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12N15/82A01H5/00
CPCC12N15/8283
Inventor 张彤周国辉
Owner SOUTH CHINA AGRI UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap