A method for detecting the quality of traditional Chinese medicine and its biologically active components based on microfluidic chip
A bioactive ingredient, microfluidic chip technology, applied in the field of medicine, can solve the problems of difficulty in discovering active ingredients of traditional Chinese medicine, long experimental procedures, and high analysis costs, so as to reduce the amount of reagents and samples, improve the quality standard of traditional Chinese medicine, and improve the analysis of traditional Chinese medicine. The effect of efficiency
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preparation example Construction
[0048] Preparation of sample solution: take a sample of traditional Chinese medicine, add solvent to extract, extract by centrifugation to remove impurity particles, and the supernatant is concentrated by centrifugation or concentrated and dried under reduced pressure to obtain the Chinese medicine extract; take an appropriate amount of the extract, accurately weigh it, and use it with the target enzyme The same buffer was dissolved, centrifuged, and the supernatant was taken for later use.
[0049] Preparation of magnetic bead-enzyme complex: take magnetic beads with a particle size of 100-150 nm, wash them with coupling buffer (PBS at pH=7.35) for 3 times, and inject them into the first channel 1 of the microfluidic chip with a micro-injection pump; Target enzymes such as thrombin / ACE are dissolved with buffer, injected into the first channel 1 that has been added with magnetic beads, and the magnetic beads are controlled to move back and forth in the channel by an external m...
Embodiment 1
[0052] Example 1 Application of thrombin and evaluation of the quality of Qishen Yiqi dripping pills
[0053] Qishen Yiqi Dropping Pills are composed of four herbs: Astragalus, Danshen, Panax notoginseng, and Jiangxiang (oil). It is mainly used for coronary heart disease, angina pectoris and other cardiovascular diseases in clinical practice. In this example, the quality of 5 different batches of Qishen Yiqi dripping pills was evaluated by thrombin inhibition rate.
[0054] A method for detecting the quality of Qishen Yiqi dripping pills and its biologically active components based on a microfluidic chip, the specific steps are as follows:
[0055] (1) Prepare a microfluidic chip (as described above).
[0056] (2) Inspection of the quality of traditional Chinese medicine:
[0057] a) Preparation of Qishenyiqi Dropping Pill Sample Solution: Take 5g of Qishenyiqi Dropping Pill sample, add 40mL of 70% methanol aqueous solution in a volume ratio, ultrasonically extract for 40min...
Embodiment 2
[0064] Example 2 Evaluation of Qishen Yiqi Dropping Pills Quality by Angiotensin-Converting Enzyme (ACE)
[0065] In this embodiment, the quality of 5 different batches of Qishen Yiqi dripping pills was evaluated by the inhibition rate of angiotensin-converting enzyme (ACE) (batch number is the same as in Example 3).
[0066] A method for detecting the quality of Qishen Yiqi dripping pills and its biologically active components based on a microfluidic chip, the specific steps are as follows:
[0067] (1) Prepare a microfluidic chip (as described above).
[0068] (2) Inspection of the quality of traditional Chinese medicine:
[0069] a) Preparation of Qishenyiqi Dropping Pill Sample Solution: Take 5g of Qishenyiqi Dropping Pill sample, add 40mL of 70% methanol aqueous solution in a volume ratio, ultrasonically extract for 40min, centrifuge the extract to remove impurity particles, and dissolve the supernatant at 50°C Centrifuge, concentrate and dry to obtain the Qishen Yiqi e...
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