Method for preparing strawberry extract for cigarette by immobilized saccharomycetes
A strawberry extract, yeast technology, applied in microorganism-based methods, biochemical equipment and methods, fixed on/in organic carriers, etc., to achieve good aroma permeability, increased applicability, and rich aroma. Effect
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[0019] Example 1
[0020] (1) Take fresh strawberries, squeeze the juice, sterilize at 121°C for 20 minutes, cool, and filter to obtain strawberry juice;
[0021] (2) Preparation of yeast suspension: inoculate yeast into broth medium. The specific formula of broth medium is (per liter): peptone 10g, beef extract powder 3g, sodium chloride 5g, and final pH 7. 3±0.1, incubate for 48 hours at a temperature of 37°C and a stirring speed of 50 rpm, take the culture solution and centrifuge at 8000 rpm, and centrifuge the wet bacteria for use;
[0022] (3) Sodium alginate-CaCl of yeast 2 Immobilization: prepare a 4% sodium alginate solution, and then add 3% of its volume to the yeast wet cells prepared in step (2) to prepare a mixed solution, and use a peristaltic pump to pass through a 1mm aperture The needle will add the prepared mixture dropwise to 3% CaCl 2 In the solution, into a ball, and CaCl 2 The solution and the pellets were stored at -4°C for 16 hours. The pellets were taken out,...
Example Embodiment
[0025] Example 2
[0026] (1) Take fresh strawberries, squeeze the juice, sterilize at 121°C for 20 minutes, cool, and filter to obtain strawberry juice;
[0027] (2) Preparation of yeast suspension: inoculate yeast into broth medium. The specific formula of broth medium is (per liter): peptone 10g, beef extract powder 3g, sodium chloride 5g, and final pH 7. 3±0.1, incubate for 48 hours at a temperature of 37°C and a stirring speed of 50 rpm, take the culture solution and centrifuge at 8000 rpm, and centrifuge the wet bacteria for use;
[0028] (3) Sodium alginate-CaCl of yeast 2 Immobilization: prepare a 5% sodium alginate solution, and then add 4% of its volume to the yeast wet cells prepared in step (2) to prepare a mixture, and use a peristaltic pump to pass through a 3mm aperture The needle will add the prepared mixture dropwise to 4% CaCl 2 In the solution, into a ball, and CaCl 2 Store the solution and pellets at -4°C for 20 hours, take out the pellets, rinse with sterile wat...
Example Embodiment
[0031] Example 3
[0032] (1) Take fresh strawberries, squeeze the juice, sterilize at 121°C for 20 minutes, cool, and filter to obtain strawberry juice;
[0033] (2) Preparation of yeast suspension: inoculate yeast into broth medium. The specific formula of broth medium is (per liter): peptone 10g, beef extract powder 3g, sodium chloride 5g, and final pH 7. 3±0.1, incubate for 48 hours at a temperature of 37°C and a stirring speed of 50 rpm, take the culture solution and centrifuge at 8000 rpm, and centrifuge the wet bacteria for use;
[0034] (3) Sodium alginate-CaCl of yeast 2 Immobilization: prepare a 5% sodium alginate solution, then add 5% of its volume to the yeast wet cells prepared in step (2) to prepare a mixed solution, and use a peristaltic pump to pass through a 4mm aperture The needle will add the prepared mixture dropwise to 6% CaCl 2 In the solution, into a ball, and CaCl 2 The solution and the pellets are stored at -4°C for 24 hours, the pellets are taken out, rinse...
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