Method for preparing strawberry extract for cigarette by immobilized saccharomycetes

A strawberry extract, yeast technology, applied in microorganism-based methods, biochemical equipment and methods, fixed on/in organic carriers, etc., to achieve good aroma permeability, increased applicability, and rich aroma. Effect

Inactive Publication Date: 2017-07-18
WUHAN HUANGHELOU FLAVOR & SPICES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there has been no report on the preparation of tobacco s

Method used

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  • Method for preparing strawberry extract for cigarette by immobilized saccharomycetes

Examples

Experimental program
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Effect test

Example Embodiment

[0019] Example 1

[0020] (1) Take fresh strawberries, squeeze the juice, sterilize at 121°C for 20 minutes, cool, and filter to obtain strawberry juice;

[0021] (2) Preparation of yeast suspension: inoculate yeast into broth medium. The specific formula of broth medium is (per liter): peptone 10g, beef extract powder 3g, sodium chloride 5g, and final pH 7. 3±0.1, incubate for 48 hours at a temperature of 37°C and a stirring speed of 50 rpm, take the culture solution and centrifuge at 8000 rpm, and centrifuge the wet bacteria for use;

[0022] (3) Sodium alginate-CaCl of yeast 2 Immobilization: prepare a 4% sodium alginate solution, and then add 3% of its volume to the yeast wet cells prepared in step (2) to prepare a mixed solution, and use a peristaltic pump to pass through a 1mm aperture The needle will add the prepared mixture dropwise to 3% CaCl 2 In the solution, into a ball, and CaCl 2 The solution and the pellets were stored at -4°C for 16 hours. The pellets were taken out,...

Example Embodiment

[0025] Example 2

[0026] (1) Take fresh strawberries, squeeze the juice, sterilize at 121°C for 20 minutes, cool, and filter to obtain strawberry juice;

[0027] (2) Preparation of yeast suspension: inoculate yeast into broth medium. The specific formula of broth medium is (per liter): peptone 10g, beef extract powder 3g, sodium chloride 5g, and final pH 7. 3±0.1, incubate for 48 hours at a temperature of 37°C and a stirring speed of 50 rpm, take the culture solution and centrifuge at 8000 rpm, and centrifuge the wet bacteria for use;

[0028] (3) Sodium alginate-CaCl of yeast 2 Immobilization: prepare a 5% sodium alginate solution, and then add 4% of its volume to the yeast wet cells prepared in step (2) to prepare a mixture, and use a peristaltic pump to pass through a 3mm aperture The needle will add the prepared mixture dropwise to 4% CaCl 2 In the solution, into a ball, and CaCl 2 Store the solution and pellets at -4°C for 20 hours, take out the pellets, rinse with sterile wat...

Example Embodiment

[0031] Example 3

[0032] (1) Take fresh strawberries, squeeze the juice, sterilize at 121°C for 20 minutes, cool, and filter to obtain strawberry juice;

[0033] (2) Preparation of yeast suspension: inoculate yeast into broth medium. The specific formula of broth medium is (per liter): peptone 10g, beef extract powder 3g, sodium chloride 5g, and final pH 7. 3±0.1, incubate for 48 hours at a temperature of 37°C and a stirring speed of 50 rpm, take the culture solution and centrifuge at 8000 rpm, and centrifuge the wet bacteria for use;

[0034] (3) Sodium alginate-CaCl of yeast 2 Immobilization: prepare a 5% sodium alginate solution, then add 5% of its volume to the yeast wet cells prepared in step (2) to prepare a mixed solution, and use a peristaltic pump to pass through a 4mm aperture The needle will add the prepared mixture dropwise to 6% CaCl 2 In the solution, into a ball, and CaCl 2 The solution and the pellets are stored at -4°C for 24 hours, the pellets are taken out, rinse...

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Abstract

The invention provides a method for preparing a strawberry extract for cigarette by immobilized saccharomycetes. The method comprises the following steps: (1) juicing fresh strawberries; (2) preparing a saccharomycetes bacterial suspension; (3) immobilizing saccharomycetes with sodium alginate-CaCl2; (4) performing fermenting reaction on the immobilized saccharomycetes and the strawberry juice; (5) filtering fermentation liquor, thereby acquiring clear fermentation liquor, and then performing alcohol precipitation and vacuum concentration, thereby acquiring the strawberry extract. The strawberry extract in cigarette provided by the invention has the functions of enriching cigarette flavor, inducing cigarette fragrance and increasing fruity sweet and fragrance. Compared with existing strawberry extractum and strawberry extract, the strawberry extract has the advantages that the fragrance in the cigarette is more abundant, the fruit flavor and sweet fragrance are more obvious and the fragrance inducing property is better.

Description

technical field [0001] The invention relates to the field of tobacco additives, in particular to a method for preparing tobacco strawberry extract by using immobilized yeast. Background technique [0002] Flavoring is a key link in the cigarette process, and it is one of the effective means to improve the determinant of product style and improve the quality of cigarette aroma. At present, flavors and fragrances for tobacco are mainly prepared through extraction and chemical synthesis of natural animals and plants. Due to limited natural plant resources and harsh conditions for chemical synthesis. With the development of biotechnology, in recent years, the use of microbial fermentation to prepare tobacco flavor raw materials provides an economical and efficient way for the production of tobacco flavor raw materials, and develops some new types of tobacco flavor raw materials that cannot be produced by traditional methods. [0003] Microbial immobilization technology is a bi...

Claims

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Application Information

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IPC IPC(8): C12P1/02C12N11/10C11B9/00C12R1/865
CPCC12P1/02C11B9/00C12N11/10
Inventor 庞登红苗丽坤黄龙张磊
Owner WUHAN HUANGHELOU FLAVOR & SPICES
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