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Improved Sabouraud agar medium and preparation method thereof

An agar medium and culture medium technology, applied in fungi and other directions, can solve the problems of uneven mixing of medium and bacterial liquid, infeasible test results, low test accuracy, etc., to avoid repeated tests and delay coagulation. time, the effect of shortening the test time

Active Publication Date: 2017-07-21
JIANGSU NAIQUE BIOLOGICAL ENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the characteristics of agar, the solidification speed is too fast at 40°C-45°C, and the solidification time is too short, resulting in uneven mixing of the medium and the bacterial solution, dense distribution of the bacterial solution, and the growth of colonies in the culture and growth. Defects, etc. do not use the disadvantages of post-calculation measurement results
As a result, the accuracy of the test is not high, the test result is not feasible, and it is necessary to repeat the test many times, wasting time

Method used

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  • Improved Sabouraud agar medium and preparation method thereof

Examples

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Effect test

Embodiment 1

[0021] An improved sandcastle agar medium, adding monoammonium glycyrrhizinate on the basis of ordinary agar medium, specifically calculated by mass fraction, including the following components: peptone 2g, glucose 4g, monoammonium glycyrrhizinate 1g, agar powder 2 g, with distilled water as the culture medium to adjust to a rated volume of 100 mL, and adjust the pH to 5.52 with 1% sodium hydroxide.

[0022] The preparation method of above-mentioned improved sandcastle agar medium comprises the steps:

[0023] A) Preparation of culture medium base liquid: take each raw material according to the required ratio of each raw material in the formula for subsequent use, dissolve peptone and glucose with distilled water as the culture medium formulation, and stir evenly to prepare the culture medium base liquid;

[0024] B) pH adjustment: adjusting the pH of the culture medium base solution prepared in step A) to a specified value;

[0025] C) preparation of medium: add glycyrrhizic...

Embodiment 2

[0028] An improved sandcastle agar medium, adding monoammonium glycyrrhizinate on the basis of ordinary agar medium, specifically calculated by mass fraction, including the following components: peptone 1g, glucose 3g, monoammonium glycyrrhizinate 1.5g, agar Powder 1.8g, with distilled water as the medium formulation to the rated volume of 100mL, with 1% sodium hydroxide to adjust the pH to 5.48.

[0029] The preparation method of the above-mentioned improved sandcastle agar medium is the same as that in Example 1.

Embodiment 3

[0031] An improved sandcastle agar medium, adding monoammonium glycyrrhizinate on the basis of ordinary agar medium, specifically calculated by mass fraction, including the following components: peptone 1.5g, glucose 5g, monoammonium glycyrrhizinate 2g, agar Powder 2.2g, with distilled water as the medium formulation to the rated volume of 100mL, with 1% sodium hydroxide to adjust the pH to 5.55.

[0032] The preparation method of the above-mentioned improved sandcastle agar medium is the same as that in Example 1.

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Abstract

The invention discloses an improved Sabouraud agar medium and a preparation method thereof, and belongs to the field of microbiological culture. The medium is basically prepared from the following materials in parts by mass: 0.5 to 2 parts of peptone, 2 to 6 parts of glucose, 1 to 3 parts of mono-ammonium glycyrrhizinate, 1.5 to 2.5 parts of agar powder, and 87 to 96 parts of distilled water. The improved Sabouraud agar medium has the advantages that the mono-ammonium glycyrrhizinate is improvably added on the basis of Sabouraud agar medium, the setting time of the medium is effectively delayed at 40 to 45 DEG C, the uniform mixing degree of bacteria solution and the medium is improved, and the phenomena not beneficial to later computed and measured results, such as colony clustering and agglomeration, overlapping and poor growth situation, caused by insufficient uniform mixing degree is effectively avoided for experiments for fungus culturing through a pouring method, so that the accuracy of experiments and experiment results are improved, repeated experiments are avoided, the testing time is shortened, error is reduced, and the medium is beneficial to the improvement of the precision degree of the experiments.

Description

technical field [0001] The invention relates to the field of microorganism culture, in particular to an improved sandcastle agar medium and a preparation method thereof. Background technique [0002] Culture medium is the nutrient substrate necessary for the growth, reproduction or accumulation of metabolites of microorganisms or animal or plant cells in the process of microbial fermentation or animal and plant cell culture. The components of a general medium include: carbon sources, nitrogen sources, minerals, and other essential substances, such as substances that cannot be synthesized by certain organisms themselves, such as certain amino acids, vitamins or nucleotides. In addition, pH buffers are often added to the medium to maintain a stable pH. [0003] The medium can be divided into natural medium, compound medium and synthetic medium according to its chemical composition. The ingredients of natural medium are all composed of natural products, such as animals, plant...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14
CPCC12N1/14
Inventor 王朝辉魏元刚钟慧易铭吴琦
Owner JIANGSU NAIQUE BIOLOGICAL ENG