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Process for producing pentanoic acid and heptanoic acid from ethanol and propionic acid

A technology of ethanol and heptanoic acid, applied in the biological field of esters and/or salts, can solve the problems such as low yield, unreliability and low efficiency of heptanoic acid esters

Inactive Publication Date: 2017-08-18
EVONIK DEGUSSA GMBH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The yield of valerate and / or heptanoate formed in this process is also very low, making it inefficient and potentially unreliable for large-scale production

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0055] Formation of valeric and heptanoic acids from propionate and ethanol by Clostridium krusei

[0056] For the bioconversion of ethanol and propionic acid into valeric and heptanoic acids, the bacterium Clostridium krusei was used. All cultivation steps were performed under anaerobic conditions in pressure-resistant glass vials that can be sealed with butyl rubber stoppers.

[0057] For pre-culture, 100ml of DMSZ52 medium (pH=7.0; 10g / L K-acetate, 0.31g / L K 2 HPO 4 , 0.23g / L KH 2 PO 4 , 0.25g / L NH4Cl, 0.20g / L MgSO 4 wxya 2 O, 1g / L yeast extract, 0.50mg / L resazurin, 10μl / L HCl (25%, 7.7M), 1.5mg / L FeCl 2 x4H 2 O, 70μg / L ZnCl 2 wxya 2 O, 100μg / L MnCl 2 x4H 2 O, 6 μg / L H 3 BO 3 , 190μg / L CoCl 2 x6 2 O, 2μg / L CuCl 2 x6 2 O, 24μg / L NiCl 2 x6 2 O, 36μg / LNa 2 MO 4 x2H 2 O, 0.5mg / L NaOH, 3μg / L NaOH 2 SeO 3 x5 2 O, 4 μg / L Na 2 WO 4 x2H 2 O, 100 μg / L vitamin B12, 80 μg / L p-aminobenzoic acid, 20 μg / L D(+) biotin, 200 μg / L niacin, 100 μg / L D-calcium pantothe...

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PUM

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Abstract

The present invention relates to a method of producing valeric acid, heptanoic acid, esters and / or salts thereof from a carbon source, the method comprising a step of contacting at least one microorganism with the carbon source in an aqueous medium, wherein the carbon source is ethanol and propionic acid and the concentration of propionic acid is about <= 10g / L.

Description

[0001] field of invention [0002] The present invention relates to a biotechnological process for the synthesis of fatty acids. In particular, the process relates to a biotechnological process for the production of at least valerenic acid, heptanoic acid, their esters and / or salts. Background technique [0003] Valerenic acid, or valeric acid, is of the formula C 5 h 10 o 2 A linear alkyl carboxylic acid. It occurs naturally in the perennial flowering plant valerian (Valeriana officinalis), from which it is named. Its main use is to synthesize its esters. Volatile valerates tend to have a pleasant odor and are used in fragrances and cosmetics. Ethyl valerate and amyl valerate because of their fruity aromas (e.g. methyl valerate-floral, ethyl valerate-fruity, especially apple, ethyl isovalerate-apple, amyl valerate-apple and Pineapple) are used as food additives. It can also be used in some applications. In particular, valeric acid, isovaleric acid and their esters are u...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/40C12P7/62C12P7/64C12P7/6436
CPCC12P7/40C12P7/62C12P7/6409C12P7/6436C07C53/126
Inventor T·哈斯S·贝克
Owner EVONIK DEGUSSA GMBH