Detection method for compound Dan Yin paste

A detection method and technology of Danyin ointment are applied in the detection field of traditional Chinese medicine preparations, which can solve problems such as adverse effects on drug quality and curative effect, harm to patients' interests, and decreased curative effect of drugs.

Active Publication Date: 2017-08-29
SICHUAN FENGCHUN PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] On the other hand, another medicinal material with the largest dosage in the prescription of this medicine is Danshen, which also accounts for a quarter of the total prescription volume. However, Danshen medicinal material is often counterfeited or confused into medicine by Gentiana chinensis, which has a negative impact on the quality and efficacy of the medicine. Gentiana is the dry root of Gentianaceae, and Salvia is the dry root and rhizome of Labiatae. However, due to the similar appearance and character of the two, in recent years, it is

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Prescription: Yinchen 240g, Danshen 240g, Bupleurum 120g, Imperata cylindrica root 120g, Radix isatidis 120g, Licorice 60g, Jujube 60g.

[0030] Preparation method: the above seven flavors, add water and decoc three times, combine the decoction, let stand, filter, the filtrate is concentrated to a relative density of 1.30 or more (heat measurement), add 240g of sucrose, heat to melt, mix, and concentrate to a relative density of 1.10 ~1.30, that is.

[0031] Properties: This product is thick and semi-fluid with a sweet and slightly bitter taste.

[0032] Check: The relative density is 1.10~1.30.

[0033] Ingredient identification and content determination:

[0034] (1) Identification of Cynanchum and Artemisia annua: take 1g of this product, add 8ml of methanol, sonicate for 20 minutes, filter, evaporate the filtrate to dryness, add 2ml of methanol to dissolve the residue, and use it as the test solution; take the scoparone reference substance , Add methanol to make a solution ...

Embodiment 2

[0043] In this embodiment, except that steps (1) to (6) in the component identification and content determination are different from the embodiment 1, the other methods are the same.

[0044] Ingredient identification and content determination:

[0045] (1) Identification of Cymbidium and Artemisia annua: Take 1g of this product, add 12ml of methanol, sonicate for 40 minutes, filter, evaporate the filtrate to dryness, add 2ml of methanol to the residue to dissolve, as the test solution; take the scoparone reference substance , Add methanol to make a solution containing 0.4mg per 1ml as the scoparone reference solution; take the artemisinin reference substance and add methanol to make a solution containing 0.2mg per 1ml as the artemisinin reference solution; draw 5μl each of the above three solutions were spotted on the same silica gel G thin-layer plate, using 60~90℃ petroleum ether: ethyl acetate: acetone = 8: 5: 3 in volume ratio as the developing agent, unfold, take out, Dry, a...

Embodiment 3

[0054] In this embodiment, except that steps (1) to (6) in the component identification and content determination are different from the embodiment 1, the other methods are the same.

[0055] (1) The identification of Yinchen and Artemisia annua is: take 1g of this product, add 10ml of methanol, sonicate for 30 minutes, filter, evaporate the filtrate to dryness, add 2ml of methanol to the residue to dissolve, as the test solution; take scoparone as control Add methanol to make a solution containing 0.4mg per 1ml as the scoparone reference solution; take the artemisinin reference substance and add methanol to make a solution containing 0.2mg per 1ml as the artemisinin reference solution; Take 5μl of each of the above three solutions, respectively spot on the same silica gel G thin-layer plate, use 60~90℃ petroleum ether: ethyl acetate: acetone = 7: 4: 2 as the developing agent in volume ratio, expand and take out , Dry, and inspect under a 365nm ultraviolet light. In the chromatog...

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PUM

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Abstract

The invention provides a detection method for compound Dan Yin paste. The method includes identification on characters and components, inspection, and content measurement. The invention aims to incomplete detection standards of the compound Dan Yin paste in the prior art, in which there are no any quality detection measures besides detections on common indices according to preparation general rules, so that the detection methods, in the prior art, has defects of monitoring medicine quality. In the method, the following items are added: identification between capillary artemisia and artemisia apiacea, identification between salvia miltiorrhiza and gentiana macrophylla, identification on radix bupleuri and licorice roots, content measurement of tanshinone IIA, and content measurement of salvianolic acid B. The method can effectively detect whether the corresponding medicine raw materials are less than standard contents or are not added due to lawless enterprises or not, and also can detect adulteration of the artemisia apiacea and gentiana macrophylla, which are liable to be confused, thereby preventing mis-use. The method can effectively guarantee the quality of the compound Dan Yin paste, ensures that the compound Dan Yin paste to be safe and effective, can guarantees clinical curative effects of the medicine and protects patient benefits.

Description

Technical field [0001] The invention relates to a detection method of traditional Chinese medicine preparations, in particular to a detection method of compound Dan Yin ointment. Background technique: [0002] Fufang Danyin Ointment is a medicine included in the second volume of "Medicine Standards for Preparations of Traditional Chinese Medicines of the Ministry of Health". The standard number is WS3-B-0339-90. The prescriptions are Yinchen, Danshen, Bupleurum, Imperata root, Radix isatidis, and Licorice Jujube is a decoction of pure Chinese medicine. It has the effects of clearing heat and dampness, detoxifying and relieving jaundice. It is clinically used to treat acute infectious hepatitis. It is currently on the market for clearing heat and dampness, detoxifying and relieving jaundice, and is used to treat acute infections. Commonly used drugs for hepatitis, but because this standard was formulated in the late 1980s and was limited to the technical level and testing instrume...

Claims

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Application Information

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IPC IPC(8): G01N30/90
CPCG01N30/90
Inventor 钟茂团黎黎温国梁林剑
Owner SICHUAN FENGCHUN PHARMA
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