Detection method for compound Dan Yin paste
A detection method and technology of Danyin ointment are applied in the detection field of traditional Chinese medicine preparations, which can solve problems such as adverse effects on drug quality and curative effect, harm to patients' interests, and decreased curative effect of drugs.
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Embodiment 1
[0029] Prescription: Yinchen 240g, Danshen 240g, Bupleurum 120g, Imperata cylindrica root 120g, Radix isatidis 120g, Licorice 60g, Jujube 60g.
[0030] Preparation method: the above seven flavors, add water and decoc three times, combine the decoction, let stand, filter, the filtrate is concentrated to a relative density of 1.30 or more (heat measurement), add 240g of sucrose, heat to melt, mix, and concentrate to a relative density of 1.10 ~1.30, that is.
[0031] Properties: This product is thick and semi-fluid with a sweet and slightly bitter taste.
[0032] Check: The relative density is 1.10~1.30.
[0033] Ingredient identification and content determination:
[0034] (1) Identification of Cynanchum and Artemisia annua: take 1g of this product, add 8ml of methanol, sonicate for 20 minutes, filter, evaporate the filtrate to dryness, add 2ml of methanol to dissolve the residue, and use it as the test solution; take the scoparone reference substance , Add methanol to make a solution ...
Embodiment 2
[0043] In this embodiment, except that steps (1) to (6) in the component identification and content determination are different from the embodiment 1, the other methods are the same.
[0044] Ingredient identification and content determination:
[0045] (1) Identification of Cymbidium and Artemisia annua: Take 1g of this product, add 12ml of methanol, sonicate for 40 minutes, filter, evaporate the filtrate to dryness, add 2ml of methanol to the residue to dissolve, as the test solution; take the scoparone reference substance , Add methanol to make a solution containing 0.4mg per 1ml as the scoparone reference solution; take the artemisinin reference substance and add methanol to make a solution containing 0.2mg per 1ml as the artemisinin reference solution; draw 5μl each of the above three solutions were spotted on the same silica gel G thin-layer plate, using 60~90℃ petroleum ether: ethyl acetate: acetone = 8: 5: 3 in volume ratio as the developing agent, unfold, take out, Dry, a...
Embodiment 3
[0054] In this embodiment, except that steps (1) to (6) in the component identification and content determination are different from the embodiment 1, the other methods are the same.
[0055] (1) The identification of Yinchen and Artemisia annua is: take 1g of this product, add 10ml of methanol, sonicate for 30 minutes, filter, evaporate the filtrate to dryness, add 2ml of methanol to the residue to dissolve, as the test solution; take scoparone as control Add methanol to make a solution containing 0.4mg per 1ml as the scoparone reference solution; take the artemisinin reference substance and add methanol to make a solution containing 0.2mg per 1ml as the artemisinin reference solution; Take 5μl of each of the above three solutions, respectively spot on the same silica gel G thin-layer plate, use 60~90℃ petroleum ether: ethyl acetate: acetone = 7: 4: 2 as the developing agent in volume ratio, expand and take out , Dry, and inspect under a 365nm ultraviolet light. In the chromatog...
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