Silicosis treatment aerosol containing human mesenchymal stem cell exosome extract and preparation method thereof

A mesenchymal stem cell and exosome technology, which is applied in the field of silicosis treatment aerosol containing human mesenchymal stem cell exosome extract and its preparation, can solve the problems of easy death, harsh survival conditions of active cells, and discounted effect of living cells To achieve the effect of promoting regeneration and repair, reducing bacterial infection and inflammatory response, and promoting differentiation of endometrial tissue

Inactive Publication Date: 2017-09-08
TIANJIN PURUI SAIER BIOLOGICAL TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there have been reports on the application of mesenchymal stem cells in the repair of lung injury and pulmonary fibrosis. However, due to the harsh survival conditions of active cells, they are easy to die, so the role played by living cells will be greatly reduced.

Method used

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  • Silicosis treatment aerosol containing human mesenchymal stem cell exosome extract and preparation method thereof
  • Silicosis treatment aerosol containing human mesenchymal stem cell exosome extract and preparation method thereof
  • Silicosis treatment aerosol containing human mesenchymal stem cell exosome extract and preparation method thereof

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preparation example Construction

[0020] The preparation method of the silicosis treatment aerosol containing human mesenchymal stem cell exosome extract of the present invention comprises the following steps:

[0021] (1) Human mesenchymal stem cell culture: P2-P6 human mesenchymal stem cells were selected from the cell bank for resuscitation, the inoculation batches were recorded as the first batch, the second batch, and the third batch, and the inoculation density was the maximum fusion 50%-60%, 35%-45%, 20%-30% of the degree; the complete medium used for culturing cells is phenol red-free DMEM / DF12 and the volume ratio concentration is 10% fetal bovine serum; the cell inoculation is completed and placed in Cultivate in a carbon dioxide incubator at a temperature of 37°C and a concentration of 5% by volume of carbon dioxide;

[0022] (2) When the fusion degree of the first batch of cells reaches 70%-80%, wash the cells twice with normal saline, and perform starvation culture with compound electrolyte inject...

Embodiment 1

[0032] (1) Human umbilical cord mesenchymal stem cell culture: P4 human umbilical cord mesenchymal stem cells were selected from the cell bank for recovery, the total number of cells was 8.4*10 7 . The inoculation batches were recorded as the first batch, the second batch, and the third batch, and the inoculation density was 60% of the maximum fusion degree (the number of inoculated cells per bottle: 9*10 6 , six bottles of cells inoculated), 40% (the number of cells inoculated in each bottle: 6*10 6 , four bottles of inoculated cells), 20% (number of inoculated cells: 3*10 6 , inoculate two bottles of cells), the culture system is 40ml. The complete medium used for culturing the cells is phenol red-free DMEM / DF12 and 10% fetal calf serum by volume, and the bottle for inoculating the cells is a T-175 culture flask. After the cell inoculation is completed, place it in a carbon dioxide incubator for cultivation at a temperature of 37°C and a volume ratio concentration of carb...

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Abstract

The invention discloses a silicosis treatment aerosol containing a human mesenchymal stem cell exosome extract and a preparation method thereof, wherein the main component of the aerosol is the high-concentration mesenchymal stem cell exosome extract. The mesenchymal stem cells can secrete various cell factors in the culture process, and the cell factors can penetrate into the basal layer of an inner membrane, promote tissue differentiation of the inner membrane, angiogenesis and growth of granulation tissues, promote regeneration and repair of damaged skin tissue structures, reduce generation of scar connective tissues and reduce bacterial infection and inflammatory reaction of wound surface tissues. According to the aerosol and the preparation method thereof, a method of continuous starvation of the mesenchymal stem cells is adopted, a high-concentration human mesenchymal extract can be obtained in a short period of time, and a big amount of stem cell exosomes can be separated from the mesenchymal extract by an exosome extractor. The aerosol containing the high-concentration mesenchymal stem cell exosome extract disclosed by the invention is suitable for the restoration and treatment of silicosis.

Description

technical field [0001] The invention relates to a stem cell exosome extract, in particular to an aerosol for treating silicosis containing a human mesenchymal stem cell exosome extract and a preparation method thereof. Background technique [0002] Mesenchymal stem cells (MSCs) are a group of heterogeneous cell populations derived from stroma, which can be obtained from most tissues of the human body. They have the ability of self-renewal, tissue repair, and immune regulation, and can differentiate to the mesoderm lineage, such as adipocytes, bone cells, chondrocytes, etc., and can also differentiate to other germ layer lineage cells, such as epidermal cells, vascular endothelium Cell Differentiation. [0003] The mesenchymal stem cell culture system contains a variety of active ingredients, such as: stem cell growth factor (SCF), fibroblast growth factor (FGF), endothelial cell growth factor (VEGF), epidermal growth factor (EGF), cell Colony-stimulating factor, interleuki...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/28A61K9/12A61P11/00A61P31/04C12N5/0775
CPCA61K35/28A61K9/008A61K9/12C12N5/0663C12N5/0665C12N5/0667C12N5/0668C12N2500/02C12N2500/84
Inventor 张冰晶鲁振宇韩洪起秦臻熊亮高婧彧徐悦黄文敬闻婧
Owner TIANJIN PURUI SAIER BIOLOGICAL TECH CO LTD
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