Application of porcine DNAH2 gene molecular marker to meat quality detection
A technology of pork and gene fragments, applied in the direction of recombinant DNA technology, DNA / RNA fragments, applications, etc., can solve the problems of affecting meat quality, not finding, affecting water loss rate, etc.
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Embodiment 1
[0022] Example 1: Detection of Acc I polymorphism of DNAH2 gene by PCR-RFLP technique.
[0023] Primer design: Use comparative genomics methods to screen out DNAH2 gene candidate SNPs sites after BLAST sequence alignment in GenBank based on the pig DNAH2 gene (GenBank accession number is XM_01398 1316), design specific primers, and use these primers to amplify Genomic DNA extracted from pig ear tissue.
[0024] The primers are: F: 5′-TGA TGC TCG TGA GTG AAA GA-3′,
[0025] R: 5'-AGG ACT GGG TGG AGG AAA T-3'.
[0026] The pig ear samples collected in the experiment mainly came from Zhenghong original breed pig farm and Chinese local breed pig farm. Some samples of DNAH2 gene experiment came from 298 pigs of 4 breeds, including 91 large white pigs from Zhenghong original breed pig farm, 72 Ningxiang pigs, 71 Shaziling pigs, and 64 Taoyuan black pigs. Use ear cutters to cut small pieces of ear-like tissue from test pigs and put them into sterilized 1.5mL Eppendorf tubes, add a...
Embodiment 2
[0032] Example 2: Distribution of DNAH2 Gene Polymorphisms in Different Pig Breeds
[0033] The gene frequency and genotype frequency of the DNAH2 gene G / C mutation site in different breeds of pigs were counted, and the chi-square test was performed on the results. The results are shown in Table 1. It can be seen from the table that there are polymorphisms in all four breeds of pigs, and the allele frequency of the C gene is higher than the G allele frequency, and the difference between the two is 0.2112, 0.7746, 0.6666, 0.0626. From this we know that in Large White pigs, Ningxiang pigs, and Taoyuan black pigs, the C gene is the dominant gene, and the CC genotype is the dominant genotype. The χ of Taoyuan black pigs found 2 2 0.05 , P>0.05, indicating that the two are in Hardy-Weinberg equilibrium. The test value χ of Large White pig and Ningxiang pig 2 >χ 2 0.001 , P<0.001, the two populations are in linkage disequilibrium, and the difference is extremely significant (...
Embodiment 3
[0040] Example 3: Association analysis between DNAH2 gene polymorphism and meat quality traits
[0041] 72 Large White pigs from Zhenghong Breeding Pig Farm were slaughtered, and eight meat quality indicators including slaughter rate, backfat thickness, eye muscle area, meat color grade, marbling, pH value, water loss rate and cooked meat rate were measured, and the Multiple comparisons and significance analysis were carried out between different genotypes of DNAH2 gene mutation sites and these meat quality traits, and the results are shown in Table 3 below. It can be seen from the table that there is no difference between different genotypes in slaughter rate, meat color grade, marbling, pH value and cooked meat rate (P>0.05). In terms of backfat thickness and eye muscle area, there was no significant difference between CC type and GC type and GG type (P>0.05), but there was a significant difference between GC type and GG type (P0.05).
[0042] Table 3 Association analysis o...
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