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A method for comprehensive utilization of aboveground stem and leaf parts of salvia miltiorrhiza

A technology of stems and leaves and Salvia miltiorrhiza is applied in the field of cellulase production by fermentation of Salvia miltiorrhiza dregs, which can solve the problems of environmental pollution of Chinese herbal medicine dregs and insufficient utilization of raw materials, and achieves the effects of improving the utilization rate of raw materials, low cost and high extraction rate

Inactive Publication Date: 2020-09-04
SHANDONG FIRST MEDICAL UNIV & SHANDONG ACADEMY OF MEDICAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, the purpose of the present invention is to solve the problem that the raw material of the traditional single component extraction method is not fully utilized, and solve the problem that the Chinese herbal medicine dregs pollute the environment

Method used

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  • A method for comprehensive utilization of aboveground stem and leaf parts of salvia miltiorrhiza
  • A method for comprehensive utilization of aboveground stem and leaf parts of salvia miltiorrhiza
  • A method for comprehensive utilization of aboveground stem and leaf parts of salvia miltiorrhiza

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preparation example Construction

[0053] (4) Preparation of cellulase crude enzyme liquid:

[0054] After the solid-state fermentation culture is completed, the fermentation culture is dried, crushed and sieved, then added with acetic acid-sodium acetate buffer solution, extracted at a constant temperature of 35-40°C for 1-1.5 hours, and the extracted extract is centrifuged to obtain a supernatant , which is the crude enzyme solution.

[0055] In step (1), the ultrasonic extraction power of the three substances is 220-250W, and the extraction time is 30-40min. Most preferably, the power of the ultrasonic extraction is 250W, and the extraction time is 40min.

[0056] For the extraction of total tanshinone: in the preferred technical scheme of the present invention, the process parameter of extraction is: the volume fraction of ethanol solution is 88%, and liquid-material ratio is 40.8: 1 (the volume / mL of liquid-material ratio is ethanol solution:material mass / g), the extraction temperature is 50°C, and the e...

Embodiment 1

[0077] Embodiment 1: the extraction process optimization of total tanshinone in the aerial stem and leaf of Salvia miltiorrhiza

[0078] 1.1 Tanshinone Ⅱ A standard curve

[0079] Accurately weigh 10 mg of tanshinone Ⅱ A, place it in a 50 ml volumetric flask, dissolve it with methanol and dilute to the mark, shake well to obtain a 0.2 mg / ml reference substance solution. Precisely measure 1.0, 2.0, 3.0, 4.0, 5.0ml of the reference solution, place them in 10ml measuring bottles, add methanol to dissolve and dilute to the mark, use methanol as a blank, and measure their absorbance at 269nm wavelength. With reference substance concentration (X) as abscissa and absorbance (Y) as ordinate, draw a standard curve ( figure 1 ).

[0080] 1.2 Optimization of total tanshinone extraction process by response surface methodology

[0081] Precisely weigh 4 or 5 parts of stems and leaves of Salvia miltiorrhiza that have been crushed and passed through a 60-mesh sieve, 0.5 g each, and use ul...

Embodiment 2

[0090] Example 2: Extraction process optimization of total salvianolic acid in Danshen medicinal residue 1

[0091] 2.1 Salvianolic acid B standard curve

[0092] Accurately measure 0.2mg / ml salvianolic acid B standard solution 0, 0.2, 0.4, 0.6, 0.8, 1.0mL respectively, put them in colorimetric tubes respectively, add water to 2.0mL precisely, add 10% nitrous acid respectively Sodium 0.5mL, shake well, then precisely add 1.0mL of 10% aluminum nitrate solution, shake well, put in dark place at room temperature for 5min, add 6.0mL of 2mol / L sodium hydroxide solution, shake well, put in dark place at room temperature for 10min , Measure the OD value at a wavelength of 500nm. Take OD value (A) as ordinate, salvianolic acid B content (X) as abscissa, draw standard curve ( image 3 ).

[0093] 2.2 Determination of sample solution

[0094] Medicinal residue 1 (0.5g) was ultrasonically extracted for a certain period of time under different ethanol volume fractions, liquid-to-solid r...

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Abstract

The invention discloses a method for comprehensively utilizing a terrestrial stem and leaf part of salvia miltiorrhiza. The method comprises the steps: (1) combined extracting total tanshinon, total phenolic acid and polysaccharide in terrestrial stems and leaves of the salvia miltiorrhiza; (2) activating psathyrella candolleana culture and preparing liquid fermentation seed solution; (3) producing cellulase from psathyrella candolleana solid state fermentation dregs 3; (4) preparing cellulase crude enzyme liquid. According to the method disclosed by the invention, single ethanol solution with volume fractions from high to low is utilized as a solvent to sequentially combined extract total tanshinon, total phenolic acid and polysaccharide from the salvia miltiorrhiza, and cellulase is extracted from the final dregs; thus, effective ingredients in the salvia miltiorrhiza are fully utilized.

Description

technical field [0001] The invention belongs to the technical field of natural product extraction, and in particular relates to a joint extraction process of total tanshinone, total salvianolic acid and polysaccharides in above-ground stems and leaves of salvia miltiorrhiza and a method for producing cellulase by fermenting salvia dregs remaining after extracting effective components. Background technique [0002] Danshen (Salvia miltiorrhiza Bge.) is a dicotyledonous Lamiaceae plant and one of the most commonly used traditional Chinese medicines for promoting blood circulation and removing blood stasis. The traditional medicinal part is the root, and the stems and leaves above the ground are generally discarded, which not only wastes a lot of resources, but also easily causes environmental pollution. Studies have shown that the stems and leaves of Salvia miltiorrhiza are rich in chemical substances such as salvianolic acids and sugars, as well as a small amount of tanshinon...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N9/42A61K36/537C12R1/645A61K135/00A61K127/00
CPCA61K36/537A61K2236/333A61K2236/39A61K2236/53C12N9/2437
Inventor 李艳玲
Owner SHANDONG FIRST MEDICAL UNIV & SHANDONG ACADEMY OF MEDICAL SCI
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