A kind of colloidal gold test paper for detecting diethylenetriaminepentaacetic acid and its chelate and its preparation and detection method
A technology of diethylenetriaminepentaacetic acid and colloidal gold test paper, which is applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of non-detection of diethylenetriaminepentaacetic acid and its chelate, large side effects, etc., and achieve accurate And the effect of sensitive detection and high yield
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Embodiment 1
[0042] Preparation of diethylenetriaminepentaacetic acid and its chelate test paper card:
[0043] 1. Synthesis and Characterization of Diethylenetriaminepentaacetic Acid-Carrier Protein Conjugates
[0044] (1) Synthesis of diethylenetriaminepentaacetic acid-keyhole limpet hemocyanin conjugate
[0045] It can be prepared according to the following steps: Dissolve 0.8-20 mg P-SCN-Bn-DTPA in 50 μL dimethyl sulfoxide to prepare 16-400 mg / mL P-SCN-Bn-DTPA; take 2.0-20.0 mg carrier protein Dissolve in 1 mL HEPES buffer solution or carbonate buffer solution to prepare 2-20 mg / mL keyhole limpet hemocyanin solution; add this hapten solution dropwise to 1 mL 2-20 mg / mL keyhole limpet blood In the blue protein solution, shake and react for 24 hours at 4°C or room temperature, pH7.5-10.5; remove unreacted low molecular substances by ultrafiltration or dialysis, and obtain diethylenetriaminepentaacetic acid-keyhole hemocyanin Protein conjugate, the complete antigen of DTPA.
[0046] 2....
Embodiment 2
[0075] In this example, the diethylenetriaminepentaacetic acid-carrier protein conjugate, namely the diethylenetriaminepentaacetic acid-molybdenum hemocyanin conjugate, is synthesized as follows: take 0.8-20 mg bifunctional chelating agent and dissolve it in 50 μL Dimethyl sulfoxide, to prepare 16-400mg / mL of p-NH 2 -Bn-DTPA; Dissolve 2.0-20.0 mg molybdenum hemocyanin in 1 mL HEPES buffer solution or carbonate buffer solution to prepare 2-20 mg / mL carrier protein solution; dissolve 1 mL 1% glutaraldehyde solution Add p-NH dropwise 2 -Bn-DTPA solution, placed overnight at room temperature to activate the amino group. Add the activated hapten solution dropwise to 1 mL of 2-20 mg / mL molybdenum hemocyanin solution, shake and react at 4°C or room temperature, pH7.5-10.5 for 24 h; use ultrafiltration or dialysis The unreacted low-molecular substances were removed by the method to obtain the diethylenetriaminepentaacetic acid-molybdenum hemocyanin conjugate, which is the complete a...
Embodiment 3
[0083] In this example, the diethylenetriaminepentaacetic acid-carrier protein conjugate, that is, the synthesis method of the diethylenetriaminepentaacetic acid-bovine serum albumin conjugate is as follows: it can be prepared according to the following steps: take 0.8-20 mg bifunctional Chelating agent was dissolved in 50 μL dimethyl sulfoxide to prepare 16-400 mg / mL P-SCN-Bn-DTPA; 2.0-20.0 mg bovine serum albumin was dissolved in 1 mL HEPES buffer solution or carbonate buffer solution, Prepare 2-20 mg / mL bovine serum albumin solution; add this hapten solution dropwise to 1 mL 2-20 mg / mL bovine serum albumin solution, at 4°C or room temperature, pH7.5-10.5 The reaction was shaken for 24 h under the condition; the unreacted low molecular substances were removed by ultrafiltration or dialysis to obtain the diethylenetriaminepentaacetic acid-carrier protein conjugate, which is the complete antigen of DTPA.
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