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Method for in-vitro quick propagation of styrax faberi

A white-flowered dragon, in vitro technology, applied in the field of rapid propagation of white-flowered dragons in vitro, can solve the problems of artificial sowing and propagation of white-flowered dragons and in-vitro culture and reproduction, and achieve the goals of shortening rooting time, increasing air permeability, and improving rooting rate Effect

Inactive Publication Date: 2017-10-10
ZHONGKAI UNIV OF AGRI & ENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, there have been no reports of artificial sowing and in vitro culture and reproduction of Baihualong

Method used

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  • Method for in-vitro quick propagation of styrax faberi
  • Method for in-vitro quick propagation of styrax faberi
  • Method for in-vitro quick propagation of styrax faberi

Examples

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Effect test

Embodiment 1

[0034] A kind of embodiment of the method for rapid propagation of white flower dragon in vitro of the present invention, comprises the following steps:

[0035] (1) Seed germination: On the ultra-clean workbench, soak the seeds of Baihualong with 75% alcohol for 40 seconds, wash them once with sterile water, peel off the outer seed shell, soak them in 0.1% mercury liter for 5 minutes, wash them with sterile water 6 times, Place on filter paper to dry, then remove the endosperm of the seeds, and finally inoculate the seeds into the corresponding germination induction medium for germination induction; each handle 10 bottles, and each bottle inoculates 3 explants; the germination induction medium Contains: MS, 1.0mg / L GA 3 , 30g / L sucrose and 7.0g / L agar, the pH value of the germination induction medium is 6.0;

[0036] (2) Subculture: the sterile seedlings obtained in step (1) are cut into stem sections with 2 nodes and inoculated into corresponding cluster bud induction mediu...

Embodiment 2

[0040] A kind of embodiment of the method for rapid propagation of white flower dragon in vitro of the present invention, comprises the following steps:

[0041] (1) Seed germination: On the ultra-clean workbench, soak the seeds of Baihualong with 75% alcohol for 60 seconds, wash them twice with sterile water, peel off the outer seed shell, soak them in 0.1% mercury liter for 6 minutes, wash them with sterile water seven times, Place them on filter paper to dry, and finally inoculate the seeds with endosperm into the corresponding germination induction medium for germination induction. 15 bottles were each treated, and 2 explants were inoculated in each bottle; the germination induction medium contained: MS, 2.0mg / L GA 3 , 30g / L sucrose and 7.0g / L agar, the pH value of the germination induction medium is 6.0;

[0042] (2) Subculture: the sterile seedlings obtained in step (1) were cut into stem segments with 2 nodes and inoculated into corresponding cluster bud induction medi...

Embodiment 3

[0046] A kind of embodiment of the method for rapid propagation of white flower dragon in vitro of the present invention, comprises the following steps:

[0047] (1) Seed germination: On the ultra-clean workbench, soak the seeds of Baihualong with 75% alcohol for 40 seconds, wash them once with sterile water, peel off the outer seed shell, soak them in 0.1% mercury liter for 4 minutes, wash them with sterile water 6 times, Place on filter paper to dry, then remove the endosperm of the seeds, and finally inoculate the seeds into the corresponding germination induction medium for germination induction; each handle 10 bottles, and each bottle inoculates 3 explants; the germination induction medium Contains: 3.0mg / L GA 3 , 30g / L sucrose and 7.0g / L agar, the pH value of the germination induction medium is 6.0;

[0048] (2) Subculture: the aseptic seedlings obtained in step (1) are cut into stems with 1 node and inoculated into corresponding cluster bud induction medium to induce c...

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Abstract

The invention discloses a method for in-vitro quick propagation of styrax faberi. The method comprises the following steps of (1) seed germinating; (2) subculture; (3) seedling strengthing and rooting culture; (4) seedling exercising and transplanting. The method for in-vitro quick propagation of the styrax faberi has the advantages that seed husk of the styrax faberi is removed, so that the air ventilating property and water permeating property are improved; endosperm is removed, so that the influence to seeds by germinating inhibiting matters and seed browning causing matters is decreased, a germinating rate and a survival rate are increased, and germinating time is shortened; a hormone type and a concentration ratio of a multiplying culture medium are optimized, so that a multiplying coefficient is increased; a rooting method, a rooting basic culture medium, the hormone type and the concentration ratio are optimized, so that a rooting rate is increased, a rooting number is increased, and rooting time is shortened; the in-vitro culture and quick propagation are realized, and a foundation is laid for the seedling supply of the styrax faberi in the fields of garden greening, edge slope restoration, medicines, greases and the like.

Description

technical field [0001] The invention relates to a method for in vitro rapid propagation of plants, in particular to a method for in vitro rapid propagation of Aurantia chinensis. Background technique [0002] Styrax faeri Perk. belongs to the genus Styrax of the family Styracaceae, and is distributed in Anhui, Hubei, Jiangsu, Zhejiang, Hunan, Jiangxi, Guangdong, Guangxi and other provinces and Taiwan. The white flower dragon tree has a beautiful shape. It blooms white flowers in late spring, which are fresh and refined. The flowers are small and the pedicels are long. When the flowers bloom, they droop, like small lanterns hanging on the branches. In Guangdong, the tree species that bloom in spring are mainly red kapok, flame wood, yellow chrysanthemum, pink Bauhinia, etc. Baihualong is one of the few native ornamental tree species with white flowers in spring and interesting shapes. It is especially suitable for the greening of communities, parks, roads, etc. Baihualong i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/005A01H4/008
Inventor 胡秀吴永清邓莎黄嘉琦姬兵兵梁韩枝
Owner ZHONGKAI UNIV OF AGRI & ENG