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Monoclonal antibody 6c9g4 and its application in the preparation of medicines for treating endotoxemia

A monoclonal antibody and culture technology, applied in the direction of antibodies, applications, antibacterial drugs, etc., can solve the problem of lack of targeted therapy drugs, and achieve the effect of improving survival rate and reducing concentration

Active Publication Date: 2020-09-08
SUZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The main reason for the high morbidity and mortality of endotoxemia is that its pathogenesis is still unclear, so there is a lack of effective targeted therapy drugs, especially the infection of antibiotic-resistant drug-resistant bacteria has become a difficult clinical problem. long term problem

Method used

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  • Monoclonal antibody 6c9g4 and its application in the preparation of medicines for treating endotoxemia
  • Monoclonal antibody 6c9g4 and its application in the preparation of medicines for treating endotoxemia
  • Monoclonal antibody 6c9g4 and its application in the preparation of medicines for treating endotoxemia

Examples

Experimental program
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Effect test

Embodiment 1

[0025] Example 1 Preparation and Identification Results of Monoclonal Antibody 6C9G4

[0026] BALB / c mice were immunized with the synthetic DHGHKHKHGHGHGHGKH polypeptide sequence, and a hybridoma cell line 6C9G4 was prepared, screened and purified by cell fusion hybridoma technology. The hybridoma cell line is preserved in the China Center for Type Culture Collection, the preservation address is Wuhan University, No. 299 Bayi Road, Wuchang District, Wuhan City, Hubei Province, the preservation date is April 26, 2017, and the preservation number is CCTCC No. C201768 .

[0027] Preparation of Monoclonal Antibody 6C9G4

[0028] (1) Inject 0.5ml of incomplete adjuvant intraperitoneally into BALB / C mice, and intraperitoneally inject 1×10 6 6C9G4 hybridoma cells, ascites was collected 7-10 days after the cells were inoculated.

[0029] (2) Monoclonal antibodies in ascites were purified and enriched by Protein G affinity purification.

[0030] The titer of the purified 6C9G4 anti...

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Abstract

The invention discloses a monoclonal antibody 6C9G4 and its application in the preparation of a drug for treating endotoxemia. The monoclonal antibody 6C9G4 produced by a hybridoma cell line with a preservation number of CCTCC No. C201768 effectively blocks the combination of HK and LPS , significantly reduce the concentration of LPS in plasma, and improve the survival rate of endotoxemia in animals. Based on this function of the monoclonal antibody 6C9G4, the monoclonal antibody 6C9G4 disclosed in the present invention has potential application prospects in controlling endotoxemia, and provides new approaches and measures for the treatment of clinical endotoxemia patients.

Description

technical field [0001] The invention relates to monoclonal antibody 6C9G4 and its application in the preparation of medicine for treating endotoxemia. Background technique [0002] Endotoxemia is a pathological manifestation caused by Gram-negative bacteria releasing a large amount of endotoxin, lipopolysaccharide (LPS) into the blood after infection. There are about 750,000 cases of endotoxemia patients in the United States every year, among which the mortality rates of severe patients and septic shock are 25-30% and 40-70%, respectively. In my country, endotoxemia caused by bacterial infection is also a major disease that seriously endangers people's lives, and its treatment costs bring a huge economic burden to patients. The main reason for the high morbidity and mortality of endotoxemia is that its pathogenesis is still unclear, so there is a lack of effective targeted therapy drugs, especially the infection of antibiotic-resistant drug-resistant bacteria has become a d...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/20C07K16/38A61K39/395A61P31/04C12N15/06
CPCA61K2039/505C07K16/38
Inventor 武艺阳艾珍
Owner SUZHOU UNIV